Q8ZT70 (PANE_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative 2-dehydropantoate 2-reductase
EC=1.1.1.169

Alternative name(s):
Ketopantoate reductase
Short name=KPA reductase
Short name=KPR

Gene names

Ordered Locus Names:

PAE3409

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827) [Reference proteome] [HAMAP]

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum › Pyrobaculum aerophilum

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Putative 2-dehydropantoate 2-reductase		PRO_0000157324
Regions
	Nucleotide binding	6 – 11	6	NADP By similarity
Sites
	Active site	158	1	Proton donor By similarity
	Binding site	86	1	NADP; via amide nitrogen By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	228	1	Substrate By similarity
	Binding site	240	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZT70 [UniParc]. Last modified February 21, 2002. Version 1. Checksum: 8A0A50DA29CD1328	FASTA	279	29,732
	10 20 30 40 50 60 MLRVIGLGAV GSLFTYFLNR AGVVPEVVQR RRCGEFLFCV EGDCEKLKFR EGGAADDVGY 70 80 90 100 110 120 TIVAVKAYDS RSVVPHLKGV AVVAQNGIGG YEEIKEAYPN SVPAVVTYGV YREGCRAELR 130 140 150 160 170 180 GVGEIYLPSA VSTLAELLER GGGRVRLVED IEPYRWLKLA VNAAINAITA LLQAPNGVII 190 200 210 220 230 240 SSPYAQTLAL EVAQEVLNVA TALGVKMPRN PVEEVLRVAS ATAKNLSSTA RDVAACAKTE 250 260 270 IDYINGAVVK YGEALGVATP VNKALFNLIK ARESLCNSG

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009441 Genomic DNA. Translation: AAL64893.1.
RefSeq	NP_560711.1. NC_003364.1.
3D structure databases
ProteinModelPortal	Q8ZT70.
ModBase	Search...
Protein-protein interaction databases
STRING	178306.PAE3409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL64893; AAL64893; PAE3409.
GeneID	1464088.
KEGG	pai:PAE3409.
Phylogenomic databases
eggNOG	COG1893.
HOGENOM	HOG000050222.
KO	K00077.
OMA	GVYREGC.
ProtClustDB	CLSK899877.
Enzyme and pathway databases
UniPathway	UPA00028; UER00004.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_PYRAE

Accession

Primary (citable) accession number: Q8ZT70

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	February 21, 2002
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program