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Protein

NAD-dependent protein deacylase 2

Gene

cobB2

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.UniRule annotation

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.UniRule annotation
NAD+ + a succinylprotein = nicotinamide + O-succinyl-ADP-ribose + a protein.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62SubstrateUniRule annotation1
Binding sitei65SubstrateUniRule annotation1
Active sitei114Proton acceptorUniRule annotation1
Metal bindingi122ZincUniRule annotation1
Metal bindingi125ZincUniRule annotation1
Metal bindingi142ZincUniRule annotation1
Metal bindingi145ZincUniRule annotation1
Binding sitei226NAD; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 37NADUniRule annotationAdd BLAST20
Nucleotide bindingi96 – 99NADUniRule annotation4
Nucleotide bindingi182 – 184NADUniRule annotation3
Nucleotide bindingi208 – 210NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacylase 2UniRule annotation (EC:3.5.1.-UniRule annotation)
Alternative name(s):
Regulatory protein SIR2 homolog 2UniRule annotation
Gene namesi
Name:cobB2UniRule annotation
Ordered Locus Names:PAE3500
OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifieri178306 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002439 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001103831 – 249NAD-dependent protein deacylase 2Add BLAST249

Interactioni

Protein-protein interaction databases

STRINGi178306.PAE3500.

Structurei

3D structure databases

ProteinModelPortaliQ8ZT00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 242Deacetylase sirtuin-typeUniRule annotationAdd BLAST242

Domaini

2 residues (Tyr-62 and Arg-65) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the sirtuin family. Class III subfamily.UniRule annotation
Contains 1 deacetylase sirtuin-type domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04248. Archaea.
COG0846. LUCA.
HOGENOMiHOG000085950.
InParanoidiQ8ZT00.
KOiK12410.
OMAiLIHMHGE.

Family and domain databases

CDDicd01412. SIRT5_Af1_CobB. 1 hit.
Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01121. Sirtuin_ClassIII. 1 hit.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_class_III.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZT00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVADLLASS RHCVVFTGAG ISAESGVPTF RGPGGLWERY KPEELATPEA
60 70 80 90 100
FARDPALVWR WYKWRQEVIY NARPSPGHYA IAELEAMGVV RGVITQNVDG
110 120 130 140 150
LHQRAGSRLV VELHGSIWRA RCVKCGSVYI LDKPVEEVPP LCRKCGGLLR
160 170 180 190 200
PDVVWFGEPL PQEAWRAAVE LASVSDVLLV VGTSGVVYPA AYIPRIAKEA
210 220 230 240
GARVVEINVE PSAITPIADV FIQGRAGEVL PRLVEEVKRR LRTRQALTP
Length:249
Mass (Da):27,352
Last modified:March 1, 2002 - v1
Checksum:i2C319244C4C7B92B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA. Translation: AAL64963.1.

Genome annotation databases

EnsemblBacteriaiAAL64963; AAL64963; PAE3500.
KEGGipai:PAE3500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA. Translation: AAL64963.1.

3D structure databases

ProteinModelPortaliQ8ZT00.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi178306.PAE3500.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL64963; AAL64963; PAE3500.
KEGGipai:PAE3500.

Phylogenomic databases

eggNOGiarCOG04248. Archaea.
COG0846. LUCA.
HOGENOMiHOG000085950.
InParanoidiQ8ZT00.
KOiK12410.
OMAiLIHMHGE.

Family and domain databases

CDDicd01412. SIRT5_Af1_CobB. 1 hit.
Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01121. Sirtuin_ClassIII. 1 hit.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_class_III.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPD2_PYRAE
AccessioniPrimary (citable) accession number: Q8ZT00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.