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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATPUniRule annotation1
Active sitei312Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi286 – 289ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:PAE3596
OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifieri178306 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000002439 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564311 – 348RNA 3'-terminal phosphate cyclaseAdd BLAST348

Interactioni

Protein-protein interaction databases

STRINGi178306.PAE3596

Structurei

3D structure databases

ProteinModelPortaliQ8ZST4
SMRiQ8ZST4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04125 Archaea
COG0430 LUCA
HOGENOMiHOG000015264
InParanoidiQ8ZST4
KOiK01974
OMAiANRPKPG
OrthoDBiPOG093Z05RD

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 2 hits
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZST4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRIDGSYG EGGGQILRTS IALSALLGRP VEIVNIRAKR ANPGLQPQHL
60 70 80 90 100
TGVKVAALLT DAEVKGAEKG STRLYFEPKT LKCGNFSIDI GTAGSISLIV
110 120 130 140 150
QTLAPILLYA PCPTQITVTG GTDVAWAPPI DYMRFVFTKV LERFGAKLSI
160 170 180 190 200
ELIRRGHYPR GGGRAVVRAE PVKRLKAVES EEFGNVVKIS GISHAVNLPP
210 220 230 240 250
HVAERQAKAA REELSKMGLD ADIAIEVRND GLGPGSGVVI WAVSDAGNVI
260 270 280 290 300
GGDSLGERGK PAETVGKEAA QKLIAVLKTR ASVDPHMADM AVLYMALAEG
310 320 330 340
RSRISTSEET MHLKTNMYII EQFLRVKFST MEKAGRYTIE VEGVGYNR
Length:348
Mass (Da):37,360
Last modified:March 1, 2002 - v1
Checksum:iB885D581A817AE1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009441 Genomic DNA Translation: AAL65029.1

Genome annotation databases

EnsemblBacteriaiAAL65029; AAL65029; PAE3596
KEGGipai:PAE3596
PATRICifig|178306.9.peg.2709

Similar proteinsi

Entry informationi

Entry nameiRTCA_PYRAE
AccessioniPrimary (citable) accession number: Q8ZST4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health