Reviewed, UniProtKB/Swiss-Prot Q8ZST4 (RTCA_PYRAE)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	PAE3596

Organism

Pyrobaculum aerophilum [Complete proteome] [HAMAP]

Taxonomic identifier

13773 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156431
Sites
	Active site	312	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZST4-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: B885D581A817AE1C	FASTA	348	37,360
	10 20 30 40 50 60 MAVRIDGSYG EGGGQILRTS IALSALLGRP VEIVNIRAKR ANPGLQPQHL TGVKVAALLT 70 80 90 100 110 120 DAEVKGAEKG STRLYFEPKT LKCGNFSIDI GTAGSISLIV QTLAPILLYA PCPTQITVTG 130 140 150 160 170 180 GTDVAWAPPI DYMRFVFTKV LERFGAKLSI ELIRRGHYPR GGGRAVVRAE PVKRLKAVES 190 200 210 220 230 240 EEFGNVVKIS GISHAVNLPP HVAERQAKAA REELSKMGLD ADIAIEVRND GLGPGSGVVI 250 260 270 280 290 300 WAVSDAGNVI GGDSLGERGK PAETVGKEAA QKLIAVLKTR ASVDPHMADM AVLYMALAEG 310 320 330 340 RSRISTSEET MHLKTNMYII EQFLRVKFST MEKAGRYTIE VEGVGYNR

References

[1]

"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009441 Genomic DNA. Translation: AAL65029.1.
RefSeq	NP_560847.1.
3D structure databases
HSSP	HSSP built from PDB template 1QMH based on UniProtKB P46849.
ModBase	Search...
Genome annotation databases
GeneID	1466163.
GenomeReviews	Gene locus PAE3596 in contig AE009441_GR.
KEGG	pai:PAE3596.
NMPDR	fig|178306.1.peg.2537.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZST4.
OMA	EIDGSYG.
Enzyme and pathway databases
BioCyc	PAER178306:PAE3596-MON.
BRENDA	6.5.1.4. 142830.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_PYRAE

Accession

Primary (citable) accession number: Q8ZST4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)