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Protein

Poly(A) polymerase I

Gene

pcnB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control.UniRule annotation

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801UniRule annotation
Active sitei82 – 821UniRule annotation
Active sitei162 – 1621UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. polynucleotide adenylyltransferase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA polyadenylation Source: InterPro
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing, Transcription

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-183-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase IUniRule annotation (EC:2.7.7.19UniRule annotation)
Short name:
PAP IUniRule annotation
Gene namesi
Name:pcnBUniRule annotation
Ordered Locus Names:STM0184
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Poly(A) polymerase IPRO_0000139095Add
BLAST

Proteomic databases

PaxDbiQ8ZRQ8.
PRIDEiQ8ZRQ8.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM0184.

Structurei

3D structure databases

ProteinModelPortaliQ8ZRQ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0617.
HOGENOMiHOG000256527.
KOiK00970.
OMAiRFMAKLD.
OrthoDBiEOG6PZX7Q.

Family and domain databases

HAMAPiMF_00957. PolyA_pol.
InterProiIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01942. pcnB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZRQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTRVANFCR KVLSREESEA EQAVARPHMT IIPREQHAIS RKDISENALK
60 70 80 90 100
VLYRLNKAGY EAYLVGGGVR DLLLGKKPKD FDVTTNATPD QVRKLFRNCR
110 120 130 140 150
LVGRRFRLAH VMFGPEIIEV ATFRGHHEGS ESDRTTSQRG QNGMLLRDNI
160 170 180 190 200
FGSIEEDAQR RDFTINSLYY SVADFTVRDY VGGMQDLQEG VIRLIGNPET
210 220 230 240 250
RYREDPVRML RAVRFAAKLN MHISPETAEP IPRLATLLND IPPARLFEES
260 270 280 290 300
LKLLQAGNGY ETYQQLREYH LFQPLFPTIT RYFTENGDSA MERIIAQVLK
310 320 330 340 350
NTDNRIRNEM RVNPAFLFAA MFWYPLLEMA QKIAQESGLA YYDAFALAMN
360 370 380 390 400
DVLDEACRSL AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LMEHPKFRAA
410 420 430 440 450
FDLLELRAQV ENNTELQRLA QWWAEFQASA PPEQKGMLNE LDDDPAPRRR
460
RSRPRKRAPR REGTV
Length:465
Mass (Da):53,922
Last modified:September 19, 2006 - v2
Checksum:i9F6C312EDDC8C376
GO

Sequence cautioni

The sequence AAL19148.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL19148.1. Different initiation.
RefSeqiNP_459189.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19148; AAL19148; STM0184.
GeneIDi1251702.
KEGGistm:STM0184.
PATRICi32378649. VBISalEnt20916_0194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL19148.1. Different initiation.
RefSeqiNP_459189.1. NC_003197.1.

3D structure databases

ProteinModelPortaliQ8ZRQ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0184.

Proteomic databases

PaxDbiQ8ZRQ8.
PRIDEiQ8ZRQ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19148; AAL19148; STM0184.
GeneIDi1251702.
KEGGistm:STM0184.
PATRICi32378649. VBISalEnt20916_0194.

Phylogenomic databases

eggNOGiCOG0617.
HOGENOMiHOG000256527.
KOiK00970.
OMAiRFMAKLD.
OrthoDBiEOG6PZX7Q.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-183-MONOMER.

Family and domain databases

HAMAPiMF_00957. PolyA_pol.
InterProiIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01942. pcnB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiPCNB_SALTY
AccessioniPrimary (citable) accession number: Q8ZRQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: September 19, 2006
Last modified: January 7, 2015
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.