Q8ZRA1 (AES_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl esterase
EC=3.1.1.-

Gene names

Name:	aes
Ordered Locus Names:	STM0490

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively regulates MalT activity by antagonizing maltotriose binding. Inhibits MelA galactosidase activity By similarity. HAMAP-Rule MF_01958
Subunit structure	Homodimer. Interacts with MalT and MelA By similarity. HAMAP-Rule MF_01958
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01958.
Sequence similarities	Belongs to the 'GDXG' lipolytic enzyme family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Serine esterase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carboxylesterase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 323

323

Acetyl esterase HAMAP-Rule MF_01958

PRO_0000239711

Sites

Active site

165

1

By similarity

Active site

262

1

By similarity

Active site

292

1

By similarity

Secondary structure

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323

Helix Strand Turn

Details...

Helix

10 – 12

3

Helix

15 – 23

9

Helix

37 – 51

15

Turn

52 – 54

3

Beta strand

59 – 65

7

Beta strand

72 – 82

11

Beta strand

86 – 90

5

Turn

94 – 96

3

Turn

100 – 103

4

Helix

104 – 114

11

Beta strand

116 – 121

6

Turn

126 – 128

3

Helix

133 – 147

15

Turn

148 – 153

6

Beta strand

157 – 164

8

Helix

166 – 181

16

Beta strand

185 – 195

11

Helix

204 – 208

5

Turn

212 – 214

3

Helix

218 – 228

11

Helix

232 – 236

5

Turn

238 – 240

3

Helix

242 – 244

3

Beta strand

254 – 259

6

Helix

265 – 277

13

Beta strand

282 – 287

6

Helix

294 – 297

4

Turn

298 – 300

3

Helix

302 – 320

19

Sequences

	Sequence		Length	Mass (Da)
	Q8ZRA1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 91A9A4EF1CCA1A45	FASTA	323	36,799
	10 20 30 40 50 60 MKPENKIPVL TRLSDEMTAV VNFQQPGLPP WPADGDIETQ RQYYLLERRF WNADAPSMTT 70 80 90 100 110 120 RTCAVPTPYG DVTTRLYSPQ PTSQATLYYL HGGGFILGNL DTHDRIMRLL ARYTGCTVIG 130 140 150 160 170 180 IDYSLSPQAR YPQAIEETVA VCSYFSQHAD EYSLNVEKIG FAGDSAGAML ALASALWLRD 190 200 210 220 230 240 KHIRCGNVIA ILLWYGLYGL QDSVSRRLFG GAWDGLTRED LDMYEKAYLR NDEDRESPWY 250 260 270 280 290 300 CLFNNDLTRD VPPCFIASAE FDPLIDDSRL LHQTLQAHQQ PCEYKMYPGT LHAFLHYSRM 310 320 MTIADDALQD GARFFMARMK TPR

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE006468 Genomic DNA. Translation: AAL19444.1.

RefSeq

NP_459485.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GA7	X-ray	1.55	A	1-323	[»]

ProteinModelPortal

Q8ZRA1.

ModBase

Search...

Protein-protein interaction databases

STRING

99287.STM0490.

Protein family/group databases

MEROPS

S09.A47.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL19444; AAL19444; STM0490.

GeneID

1252010.

KEGG

stm:STM0490.

PATRIC

32379325. VBISalEnt20916_0524.

Phylogenomic databases

eggNOG

COG0657.

HOGENOM

HOG000117644.

KO

K01066.

OMA

MKPENKS.

ProtClustDB

PRK10162.

Enzyme and pathway databases

BioCyc

SENT99287:GCTI-493-MONOMER.

Family and domain databases

HAMAP

MF_01958. Acetyl_esterase.

InterPro

IPR013094. AB_hydrolase_3.
IPR023508. Acetyl_esterase_GDXG_lipolytic.
IPR002168. Lipase_GDXG_AS.
[Graphical view]

Pfam

PF07859. Abhydrolase_3. 1 hit.
[Graphical view]

PROSITE

PS01173. LIPASE_GDXG_HIS. False negative.
PS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8ZRA1.

Entry information

Entry name

AES_SALTY

Accession

Primary (citable) accession number: Q8ZRA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program