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Protein

Putative glutamate--cysteine ligase 2

Gene

ybdK

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamate--cysteine ligase 2UniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-glutamylcysteine synthetase 2UniRule annotation
Short name:
GCS 2UniRule annotation
Short name:
Gamma-GCS 2UniRule annotation
Gene namesi
Name:ybdK
Ordered Locus Names:STM0583
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002182171 – 372Putative glutamate--cysteine ligase 2Add BLAST372

Proteomic databases

PaxDbiQ8ZR41.
PRIDEiQ8ZR41.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM0583.

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 23Combined sources10
Turni25 – 27Combined sources3
Helixi37 – 41Combined sources5
Helixi42 – 44Combined sources3
Beta strandi47 – 52Combined sources6
Beta strandi58 – 62Combined sources5
Beta strandi66 – 68Combined sources3
Helixi69 – 89Combined sources21
Beta strandi93 – 95Combined sources3
Helixi119 – 123Combined sources5
Helixi124 – 127Combined sources4
Beta strandi134 – 140Combined sources7
Beta strandi142 – 144Combined sources3
Helixi145 – 155Combined sources11
Helixi158 – 165Combined sources8
Helixi182 – 186Combined sources5
Helixi201 – 211Combined sources11
Beta strandi214 – 216Combined sources3
Helixi220 – 222Combined sources3
Beta strandi226 – 230Combined sources5
Turni231 – 234Combined sources4
Beta strandi235 – 242Combined sources8
Helixi247 – 267Combined sources21
Helixi274 – 279Combined sources6
Helixi280 – 289Combined sources10
Beta strandi294 – 297Combined sources4
Turni299 – 301Combined sources3
Beta strandi304 – 306Combined sources3
Helixi307 – 317Combined sources11
Helixi319 – 325Combined sources7
Helixi328 – 339Combined sources12
Helixi344 – 353Combined sources10
Helixi358 – 368Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TT4X-ray2.80A/B1-372[»]
ProteinModelPortaliQ8ZR41.
SMRiQ8ZR41.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZR41.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106KXD. Bacteria.
COG2170. LUCA.
HOGENOMiHOG000220942.
KOiK06048.
OMAiAFPMSGR.
PhylomeDBiQ8ZR41.

Family and domain databases

HAMAPiMF_01609. Glu_cys_ligase_2. 1 hit.
InterProiIPR006336. GCS2.
IPR011793. YbdK.
[Graphical view]
PfamiPF04107. GCS2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02050. gshA_cyan_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZR41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNDFHVSE PYTLGIELEM QVINPPGYDL SQDSSTLIDA VKPQLTAGEI
60 70 80 90 100
KHDITESMLE MATGVCRDID QAAAQLSAMQ HVILQAASEH HLGICGGGTH
110 120 130 140 150
PFQKWQRQEV CDNERYQRTL ENFGYLIQQA TVFGQHVHVG CANGDDAIYL
160 170 180 190 200
LHGLSHFVPH FIALSAASPY MQGADTRFAC ARLNIFSAFP DNGPMPWVSN
210 220 230 240 250
WQEFAGLFRR LSYTTMIDSI KDLHWDIRPS PAFGTVEVRV MDTPLTLDHA
260 270 280 290 300
INMAGLIQAT AHWLLTERPF KPQEQDYLLY KFNRFQACRY GLEGVLTDAY
310 320 330 340 350
TGDRRRLADD TLRLLDNVTP SARKLGADSA IDALRLQVKK GGNEAQYMRE
360 370
FIADGGSLIG LVQKHCEIWA GQ
Length:372
Mass (Da):41,597
Last modified:March 1, 2002 - v1
Checksum:iB448A5897F5CA496
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL19534.1.
RefSeqiNP_459575.1. NC_003197.1.
WP_001196903.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19534; AAL19534; STM0583.
GeneIDi1252103.
KEGGistm:STM0583.
PATRICi32379511. VBISalEnt20916_0615.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL19534.1.
RefSeqiNP_459575.1. NC_003197.1.
WP_001196903.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TT4X-ray2.80A/B1-372[»]
ProteinModelPortaliQ8ZR41.
SMRiQ8ZR41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0583.

Proteomic databases

PaxDbiQ8ZR41.
PRIDEiQ8ZR41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19534; AAL19534; STM0583.
GeneIDi1252103.
KEGGistm:STM0583.
PATRICi32379511. VBISalEnt20916_0615.

Phylogenomic databases

eggNOGiENOG4106KXD. Bacteria.
COG2170. LUCA.
HOGENOMiHOG000220942.
KOiK06048.
OMAiAFPMSGR.
PhylomeDBiQ8ZR41.

Miscellaneous databases

EvolutionaryTraceiQ8ZR41.

Family and domain databases

HAMAPiMF_01609. Glu_cys_ligase_2. 1 hit.
InterProiIPR006336. GCS2.
IPR011793. YbdK.
[Graphical view]
PfamiPF04107. GCS2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02050. gshA_cyan_rel. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGCS2_SALTY
AccessioniPrimary (citable) accession number: Q8ZR41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.