Reviewed, UniProtKB/Swiss-Prot Q8ZQU2 (DHSB_SALTY)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase iron-sulfur subunit
    EC=1.3.99.1

Gene names

Name:	sdhB
Ordered Locus Names:	STM0735

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth By similarity.
Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster. Binds 1 3Fe-4S cluster. Binds 1 4Fe-4S cluster.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein By similarity.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Succinate dehydrogenase iron-sulfur subunit		PRO_0000158692
Regions
	Domain	1 – 97	97	2Fe-2S ferredoxin-type
	Domain	139 – 169	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	55	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	60	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	63	1	Iron-sulfur 1 (2Fe-2S) Potential
	Metal binding	75	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	149	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	152	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	155	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	159	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	206	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	212	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	216	1	Iron-sulfur 2 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZQU2-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 7628F9F9F95611F3	FASTA	238	26,733
	10 20 30 40 50 60 MKLEFSIYRY NPDVDNAPRM QDYTLEGEEG RDMMLLDALI QLKEKDPSLS FRRSCREGVC 70 80 90 100 110 120 GSDGLNMNGK NGLACITPIS ALTQPGKKIV IRPLPGLPVI RDLVVDMGQF YAQYEKIKPY 130 140 150 160 170 180 LLNNGQNPPA REHLQMPEQR EKLDGLYECI LCACCSTSCP SFWWNPDKFI GPAGLLAAYR 190 200 210 220 230 FLIDSRDTET DSRLEGMSDA FSVFRCHSIM NCVSVCPKGL NPTRAIGHIK SMLLQRSA

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008730 Genomic DNA. Translation: AAL19679.1. Different initiation.
RefSeq	NP_459720.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P07014.
SMR	Q8ZQU2. Positions 1-238.
ModBase	Search...
Genome annotation databases
GeneID	1252255.
GenomeReviews	Gene locus STM0735 in contig AE006468_GR.
KEGG	stm:STM0735.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZQU2.
OMA	Q8ZQU2. LIVDMEP.
Enzyme and pathway databases
BioCyc	STYP99287:STM0735-MON.
BRENDA	1.3.99.1. 2.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DH/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSB_SALTY

Accession

Primary (citable) accession number: Q8ZQU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 6, 2002
Last modified:	June 16, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)