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Protein

Glyoxylate/hydroxypyruvate reductase A

Gene

ghrA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively.UniRule annotation

Catalytic activityi

Glycolate + NADP+ = glyoxylate + NADPH.UniRule annotation
D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei227UniRule annotation1
Active sitei275Proton donorUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Glyoxylate/hydroxypyruvate reductase AUniRule annotation (EC:1.1.1.79UniRule annotation, EC:1.1.1.81UniRule annotation)
Alternative name(s):
2-ketoacid reductaseUniRule annotation
Gene namesi
Name:ghrAUniRule annotation
Ordered Locus Names:STM1135
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003483721 – 312Glyoxylate/hydroxypyruvate reductase AAdd BLAST312

Proteomic databases

PaxDbiQ8ZQ30.
PRIDEiQ8ZQ30.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM1135.

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 6Combined sources6
Beta strandi8 – 10Combined sources3
Helixi12 – 22Combined sources11
Beta strandi26 – 30Combined sources5
Beta strandi40 – 46Combined sources7
Helixi49 – 52Combined sources4
Beta strandi58 – 65Combined sources8
Helixi68 – 76Combined sources9
Helixi78 – 80Combined sources3
Beta strandi87 – 89Combined sources3
Helixi96 – 111Combined sources16
Helixi114 – 122Combined sources9
Helixi134 – 136Combined sources3
Beta strandi139 – 142Combined sources4
Helixi146 – 156Combined sources11
Turni157 – 159Combined sources3
Beta strandi162 – 168Combined sources7
Beta strandi176 – 180Combined sources5
Helixi181 – 189Combined sources9
Beta strandi192 – 196Combined sources5
Helixi202 – 204Combined sources3
Helixi210 – 213Combined sources4
Beta strandi220 – 224Combined sources5
Helixi228 – 230Combined sources3
Helixi233 – 241Combined sources9
Beta strandi244 – 251Combined sources8
Beta strandi254 – 257Combined sources4
Helixi263 – 266Combined sources4
Beta strandi270 – 272Combined sources3
Helixi282 – 298Combined sources17
Turni308 – 311Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KBOX-ray2.14A/B/C/D1-312[»]
3PP8X-ray2.10A1-312[»]
ProteinModelPortaliQ8ZQ30.
SMRiQ8ZQ30.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZQ30.

Family & Domainsi

Sequence similaritiesi

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105JPG. Bacteria.
COG0111. LUCA.
HOGENOMiHOG000136694.
KOiK12972.
OMAiHKAIPQA.
PhylomeDBiQ8ZQ30.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01666. 2_Hacid_dh_C_GhrA. 1 hit.
InterProiIPR006140. D-isomer_DH_NAD-bd.
IPR023514. GhrA.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZQ30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIIFYHPTF NAAWWVNALE KALPHARVRE WKVGDNNPAD YALVWQPPVE
60 70 80 90 100
MLAGRRLKAV FVLGAGVDAI LSKLNAHPEM LDASIPLFRL EDTGMGLQMQ
110 120 130 140 150
EYAVSQVLHW FRRFDDYQAL KNQALWKPLP EYTREEFSVG IMGAGVLGAK
160 170 180 190 200
VAESLQAWGF PLRCWSRSRK SWPGVESYVG REELRAFLNQ TRVLINLLPN
210 220 230 240 250
TAQTVGIINS ELLDQLPDGA YVLNLARGVH VQEADLLAAL DSGKLKGAML
260 270 280 290 300
DVFSQEPLPQ ESPLWRHPRV AMTPHIAAVT RPAEAIDYIS RTITQLEKGE
310
PVTGQVDRAR GY
Length:312
Mass (Da):35,034
Last modified:March 1, 2002 - v1
Checksum:iA46D708D25A7AD11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20065.1.
RefSeqiNP_460106.1. NC_003197.1.
WP_000402556.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20065; AAL20065; STM1135.
GeneIDi1252653.
KEGGistm:STM1135.
PATRICi32380715. VBISalEnt20916_1202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20065.1.
RefSeqiNP_460106.1. NC_003197.1.
WP_000402556.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KBOX-ray2.14A/B/C/D1-312[»]
3PP8X-ray2.10A1-312[»]
ProteinModelPortaliQ8ZQ30.
SMRiQ8ZQ30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1135.

Proteomic databases

PaxDbiQ8ZQ30.
PRIDEiQ8ZQ30.

Protocols and materials databases

DNASUi1252653.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20065; AAL20065; STM1135.
GeneIDi1252653.
KEGGistm:STM1135.
PATRICi32380715. VBISalEnt20916_1202.

Phylogenomic databases

eggNOGiENOG4105JPG. Bacteria.
COG0111. LUCA.
HOGENOMiHOG000136694.
KOiK12972.
OMAiHKAIPQA.
PhylomeDBiQ8ZQ30.

Miscellaneous databases

EvolutionaryTraceiQ8ZQ30.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01666. 2_Hacid_dh_C_GhrA. 1 hit.
InterProiIPR006140. D-isomer_DH_NAD-bd.
IPR023514. GhrA.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGHRA_SALTY
AccessioniPrimary (citable) accession number: Q8ZQ30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.