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Protein

Beta-hexosaminidase

Gene

nagZ

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides. Plays a role in beta-lactam antibiotic resistance via its role in generating anhydro-N-acetylmuramic acid-linked peptides; these peptides function as signaling molecules that induce high-level expression of the beta-lactamase AmpC.UniRule annotation2 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.UniRule annotation2 Publications

Pathwayi: peptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62Substrate1
Binding sitei70SubstrateUniRule annotation1
Binding sitei133Substrate1
Sitei174Important for catalytic activityUniRule annotation1
Active sitei176Proton donor/acceptor1 Publication1
Active sitei248NucleophileUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00544.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidaseUniRule annotation (EC:3.2.1.52UniRule annotation)
Alternative name(s):
Beta-N-acetylhexosaminidaseUniRule annotation
N-acetyl-beta-glucosaminidaseUniRule annotation
Gene namesi
Name:nagZUniRule annotation
Ordered Locus Names:STM1209
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176H → G: Reduced enzyme activity. 1
Mutagenesisi248D → N: Abolishes enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002107971 – 341Beta-hexosaminidaseAdd BLAST341

Proteomic databases

PaxDbiQ8ZQ06.
PRIDEiQ8ZQ06.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM1209.

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi9 – 12Combined sources4
Helixi15 – 21Combined sources7
Beta strandi26 – 31Combined sources6
Helixi33 – 35Combined sources3
Helixi39 – 52Combined sources14
Beta strandi58 – 61Combined sources4
Beta strandi63 – 65Combined sources3
Beta strandi68 – 70Combined sources3
Helixi83 – 109Combined sources27
Turni110 – 112Combined sources3
Turni128 – 130Combined sources3
Helixi131 – 133Combined sources3
Helixi139 – 156Combined sources18
Beta strandi161 – 165Combined sources5
Beta strandi175 – 178Combined sources4
Helixi186 – 191Combined sources6
Helixi193 – 202Combined sources10
Beta strandi207 – 211Combined sources5
Turni217 – 219Combined sources3
Helixi224 – 226Combined sources3
Helixi228 – 231Combined sources4
Helixi232 – 238Combined sources7
Beta strandi243 – 249Combined sources7
Helixi250 – 252Combined sources3
Helixi256 – 258Combined sources3
Helixi261 – 271Combined sources11
Beta strandi274 – 278Combined sources5
Helixi282 – 291Combined sources10
Helixi298 – 303Combined sources6
Helixi311 – 316Combined sources6
Helixi318 – 339Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GVFX-ray1.35A/B1-341[»]
4GVGX-ray1.70A/B1-341[»]
4GVHX-ray1.45A/B1-341[»]
4GVIX-ray1.55A/B1-341[»]
4HZMX-ray1.45A/B1-341[»]
ProteinModelPortaliQ8ZQ06.
SMRiQ8ZQ06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 164Substrate binding2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QPA. Bacteria.
COG1472. LUCA.
HOGENOMiHOG000248526.
KOiK01207.
OMAiEHDMAIF.
PhylomeDBiQ8ZQ06.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
HAMAPiMF_00364. NagZ. 1 hit.
InterProiIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZQ06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPVMLNVEG CELDAEEREI LAHPLVGGLI LFTRNYHDPE QLRELVRQIR
60 70 80 90 100
AASRNHLVVA VDQEGGRVQR FREGFTRLPA AQSFFALHGL EEGGRLAQEA
110 120 130 140 150
GWLMASEMIA MDIDISFAPV LDVGHISAAI GERSYHADPA KALAMATRFI
160 170 180 190 200
DGMHDAGMKT TGKHFPGHGA VTADSHKETP CDPRPETDIR GKDMSVFRTL
210 220 230 240 250
ISENKLDAIM PAHVIYRAID PRPASGSPYW LKTVLRQELG FDGVIFSDDL
260 270 280 290 300
SMEGAAIMGS YAERAQASLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV
310 320 330 340
TRLYHKGSFS RRELMDSARW KTASAQLNQL HERWQEEKAG H
Length:341
Mass (Da):37,698
Last modified:March 1, 2002 - v1
Checksum:iE07E83487336A300
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20138.1.
RefSeqiNP_460179.1. NC_003197.1.
WP_000529340.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20138; AAL20138; STM1209.
GeneIDi1252727.
KEGGistm:STM1209.
PATRICi32380867. VBISalEnt20916_1278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20138.1.
RefSeqiNP_460179.1. NC_003197.1.
WP_000529340.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GVFX-ray1.35A/B1-341[»]
4GVGX-ray1.70A/B1-341[»]
4GVHX-ray1.45A/B1-341[»]
4GVIX-ray1.55A/B1-341[»]
4HZMX-ray1.45A/B1-341[»]
ProteinModelPortaliQ8ZQ06.
SMRiQ8ZQ06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1209.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Proteomic databases

PaxDbiQ8ZQ06.
PRIDEiQ8ZQ06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20138; AAL20138; STM1209.
GeneIDi1252727.
KEGGistm:STM1209.
PATRICi32380867. VBISalEnt20916_1278.

Phylogenomic databases

eggNOGiENOG4107QPA. Bacteria.
COG1472. LUCA.
HOGENOMiHOG000248526.
KOiK01207.
OMAiEHDMAIF.
PhylomeDBiQ8ZQ06.

Enzyme and pathway databases

UniPathwayiUPA00544.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
HAMAPiMF_00364. NagZ. 1 hit.
InterProiIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAGZ_SALTY
AccessioniPrimary (citable) accession number: Q8ZQ06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.