Q8ZPU9 (ASTB_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-succinylarginine dihydrolase
EC=3.5.3.23

Gene names

Name:	astB
Ordered Locus Names:	STM1306

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2 By similarity. HAMAP MF_01172
Catalytic activity	N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2. HAMAP MF_01172
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 2/5. HAMAP MF_01172
Subunit structure	Homodimer By similarity. HAMAP MF_01172
Induction	By nitrogen and carbon starvation, and arginine, via the ArgR and Crp transcriptional regulators. Ref.2
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-succinylarginine dihydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	N-succinylarginine dihydrolase HAMAP MF_01172		PRO_0000262373
Regions
	Region	19 – 28	10	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	174	1	By similarity
	Active site	248	1	By similarity
	Active site	365	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	359	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZPU9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: B1C7296EBD1D7C9C	FASTA	447	49,192
	10 20 30 40 50 60 MTAHEVNFDG LVGLTHHYAG LSFGNEASTR HRFQVSNPRL AVKQGLLKMK ALADAGFPQA 70 80 90 100 110 120 VIPPHERPFI PALRQLGFTG SDEQILDKVA RQAPRWLSSV SSASPMWVAN AATVCPSADA 130 140 150 160 170 180 LDGKVHLTVA NLNNKFHRAL EAPVTEALLR AIFRDESQFS VHSALPQVAL LGDEGAANHN 190 200 210 220 230 240 RLGGEYGSAG VQLFVYGREE ENEIRPARYP ARQSREASEA VARLNQVNPQ QVIFAQQNPE 250 260 270 280 290 300 VIDQGVFHND VIAVSNRQVL FCHEAAFARQ KVLINQLRTR VDGFMAIEVP AGEVSVSDAV 310 320 330 340 350 360 ATYLFNSQLL SRNDGLMLLV LPRECQDHVG VWRYLNKLVA EDNPISAMQV FDLRESMANG 370 380 390 400 410 420 GGPACLRLRV VLTEEERRAV NPAVMMNDAL FTALNAWADR YYRDRLTAAD LADPLLLREG 430 440 REALDVLTRL LDLGSVYPFQ QTGAADG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"Role of ArgR in activation of the ast operon, encoding enzymes of the arginine succinyltransferase pathway in Salmonella typhimurium." Lu C.-D., Abdelal A.T. J. Bacteriol. 181:1934-1938(1999) [PubMed: 10074092] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL20231.1.
RefSeq	NP_460272.1. NC_003197.1.
3D structure databases
HSSP	HSSP built from PDB template 1YNF based on UniProtKB P76216.
ProteinModelPortal	Q8ZPU9.
SMR	Q8ZPU9. Positions 3-441.
ModBase	Search...
Proteomic databases
PRIDE	Q8ZPU9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1252824.
GenomeReviews	Gene locus STM1306 in contig AE006468_GR.
KEGG	stm:STM1306.
NMPDR	fig|99287.1.peg.1264.
PATRIC	32381091. VBISalEnt20916_1388.
Phylogenomic databases
HOGENOM	HBG289862.
OMA	HFAHHPA.
ProtClustDB	PRK13281.
Enzyme and pathway databases
BioCyc	STYP99287:STM1306-MONOMER.
Family and domain databases
HAMAP	MF_01172. AstB. [Tree]
InterPro	IPR007079. SuccinylArg_d-Hdrlase_AstB. [Graphical view]
Gene3D	G3DSA:3.75.10.20. SuccinylArg_di_hydro. 1 hit.
KO	K01484.
Pfam	PF04996. AstB. 1 hit. [Graphical view]
TIGRFAMs	TIGR03241. Arg_catab_astB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTB_SALTY

Accession

Primary (citable) accession number: Q8ZPU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program