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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:STM1310
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521911 – 275NH(3)-dependent NAD(+) synthetaseAdd BLAST275

Proteomic databases

PaxDbiQ8ZPU5.
PRIDEiQ8ZPU5.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM1310.

Structurei

Secondary structure

1275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Helixi19 – 36Combined sources18
Beta strandi42 – 47Combined sources6
Helixi51 – 71Combined sources21
Beta strandi77 – 82Combined sources6
Beta strandi85 – 87Combined sources3
Helixi92 – 101Combined sources10
Beta strandi104 – 108Combined sources5
Helixi112 – 124Combined sources13
Helixi131 – 152Combined sources22
Beta strandi155 – 158Combined sources4
Helixi163 – 166Combined sources4
Turni167 – 169Combined sources3
Turni173 – 177Combined sources5
Turni183 – 186Combined sources4
Helixi189 – 199Combined sources11
Helixi203 – 205Combined sources3
Helixi231 – 238Combined sources8
Helixi245 – 257Combined sources13
Helixi259 – 262Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HMQX-ray1.90A1-275[»]
ProteinModelPortaliQ8ZPU5.
SMRiQ8ZPU5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZPU5.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
KOiK01916.
OMAiAKIIEGW.
PhylomeDBiQ8ZPU5.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZPU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQQEIIQA LGAKPHINPE EEIRRSVDFL KAYLKTYPFL KSLVLGISGG
60 70 80 90 100
QDSTLAGKLS QMAIAELREE TGDNALQFIA VRLPYGVQAD EQDCQDAIAF
110 120 130 140 150
IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA
160 170 180 190 200
GMTHGVVVGT DHAAEAITGF FTKYGDGGTD INPLHRLNKR QGKQLLAALG
210 220 230 240 250
CPEHLYKKVP TADLEDDRPS LPDEAALGVT YDNIDDYLEG KTLDPAIAKT
260 270
IEGWYVKTEH KRRLPITVFD DFWKR
Length:275
Mass (Da):30,484
Last modified:March 1, 2002 - v1
Checksum:i6E0F3AB5B812B6EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20235.1.
RefSeqiNP_460276.1. NC_003197.1.
WP_000174981.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20235; AAL20235; STM1310.
GeneIDi1252828.
KEGGistm:STM1310.
PATRICi32381099. VBISalEnt20916_1392.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20235.1.
RefSeqiNP_460276.1. NC_003197.1.
WP_000174981.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HMQX-ray1.90A1-275[»]
ProteinModelPortaliQ8ZPU5.
SMRiQ8ZPU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1310.

Proteomic databases

PaxDbiQ8ZPU5.
PRIDEiQ8ZPU5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20235; AAL20235; STM1310.
GeneIDi1252828.
KEGGistm:STM1310.
PATRICi32381099. VBISalEnt20916_1392.

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
KOiK01916.
OMAiAKIIEGW.
PhylomeDBiQ8ZPU5.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Miscellaneous databases

EvolutionaryTraceiQ8ZPU5.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_SALTY
AccessioniPrimary (citable) accession number: Q8ZPU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.