NAD-dependent malic enzyme - Salmonella typhimurium

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Reviewed, UniProtKB/Swiss-Prot Q8ZPE8 (MAO1_SALTY)

Last modified June 16, 2009. Version 43. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	STM1566

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD⁺ = pyruvate + CO₂ + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

565

NAD-dependent malic enzyme HAMAP MF_01619

PRO_0000160231

Sites

Active site

1

Proton donor By similarity

Active site

1

Proton acceptor By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Site

1

Important for activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8ZPE8-1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 0A98386BCEC7FBCE
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565

62,896

        10         20         30         40         50         60 
METTTKKARS LYIPYAGPVL LEFPLLNKGS AFSVEERRNF NLSGLLPEVV ESIEEQAERA 

        70         80         90        100        110        120 
WLQYQGFKTE IDKHIYLRNI QDTNETLFYR LVQNHLEEMM PVIYTPTVGA ACERFSEIYR 

       130        140        150        160        170        180 
RARGVFISYP NRHNMDDILQ NVPNHNIKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 

       190        200        210        220        230        240 
ACGGISPAYT LPVVLDVGTN NQQLLNDPLY MGWRHPRITD DEYYAFVDEF IQAVKQRWPD 

       250        260        270        280        290        300 
ILLQFEDFAQ KNAMPLLTRY RDEICSFNDD IQGTAAVTVG TLIAASRAAG SQLSEQKIVF 

       310        320        330        340        350        360 
LGAGSAGCGI AEQIIAQTQR EGLSEDAARQ NVFMVDRFGL LTDRMPNLLP FQAKLVQKCD 

       370        380        390        400        410        420 
NLQHWDTEND VLSLLDVVRN VKPDILIGVS GQTGLFTEEI IREMHKHCPR PIVMPLSNPT 

       430        440        450        460        470        480 
SRVEATPQDI IAWTEGNALV ATGSPFSPVI WKDKVYPIAQ CNNAYIFPGI GLGVIASGAS 

       490        500        510        520        530        540 
RITDEMLMSA SETLAKHSPL VNNGEGLVLP ALKDIQVVSR AIAFAVGKMA QQQGVAVKTS 

       550        560 
AEALQQAIDD NFWKPEYRDY RRTSI

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.

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Wilson R.K.
Nature 413:852-856(2001) [PubMed: 11677609] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
	AE008768 Genomic DNA. Translation: AAL20484.1.
RefSeq	NP_460525.1.
3D structure databases
HSSP	HSSP built from PDB template 1O0S based on UniProtKB P27443.
ModBase	Search...
Genome annotation databases
GeneID	1253084.
GenomeReviews	Gene locus STM1566 in contig AE006468_GR.
KEGG	stm:STM1566.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZPE8.
OMA	Q8ZPE8. IQDNNET.
Enzyme and pathway databases
BioCyc	STYP99287:STM1566-MON.
BRENDA	1.1.1.38. 2.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_SALTY

Accession

Primary (citable) accession number: Q8ZPE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents