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Protein

L-methionine sulfoximine/L-methionine sulfone acetyltransferase

Gene

yncA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the resistance against the toxic effects of L-methionine sulfoximine (MSX), a rare amino acid which inhibits glutamine synthetase (GlnA). Catalyzes the acetylation of MSX. It can also use L-methionine sulfone (MSO).1 Publication

Catalytic activityi

Acetyl-CoA + L-methionine sulfoximine = CoA + N-acetyl-L-methionine sulfoximine.1 Publication
Acetyl-CoA + L-methionine sulfone = CoA + N-acetyl-L-methionine sulfone.1 Publication

Kineticsi

Kcat is 35 sec(-1) for acetyltransferase activity with MSX as substrate (at pH 7.2 and 30 degrees Celsius). Kcat is 31 sec(-1) for acetyltransferase activity with MSO as substrate (at pH 7.2 and 30 degrees Celsius). Kcat is 23 sec(-1) for acetyltransferase activity with acetyl-CoA as substrate (at pH 7.2 and 30 degrees Celsius).1 Publication

  1. KM=156 µM for acetyl-CoA (at pH 7.2 and 30 degrees Celsius)1 Publication
  2. KM=230 µM for MSO (at pH 7.2 and 30 degrees Celsius)1 Publication
  3. KM=576 µM for MSX (at pH 7.2 and 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei124 – 1241Acetyl-CoA1 Publication
    Binding sitei133 – 1331Acetyl-CoA1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-1600-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-methionine sulfoximine/L-methionine sulfone acetyltransferase1 Publication (EC:2.3.1.-1 Publication)
    Alternative name(s):
    Methionine derivative detoxifier A1 Publication
    Short name:
    MDDA1 Publication
    Gene namesi
    Name:yncA
    Ordered Locus Names:STM1590
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    In minimal medium, cells lacking this gene grow normally with MSO, but growth is inhibited by MSX. In rich medium, growth of cells lacking this gene are partially inhibited by MSX, only when its concentration is at least 50 µM.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi82 – 821E → Q: Loss of acetyltransferase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 171171L-methionine sulfoximine/L-methionine sulfone acetyltransferasePRO_0000433346Add
    BLAST

    Proteomic databases

    PaxDbiQ8ZPD3.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    STRINGi99287.STM1590.

    Structurei

    Secondary structure

    1
    171
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 54Combined sources
    Helixi8 – 103Combined sources
    Helixi11 – 2313Combined sources
    Turni26 – 294Combined sources
    Helixi36 – 4914Combined sources
    Beta strandi53 – 586Combined sources
    Beta strandi61 – 7414Combined sources
    Helixi75 – 773Combined sources
    Beta strandi80 – 878Combined sources
    Helixi89 – 913Combined sources
    Helixi96 – 11015Combined sources
    Beta strandi114 – 1218Combined sources
    Helixi125 – 1339Combined sources
    Beta strandi137 – 14812Combined sources
    Beta strandi151 – 16111Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DR6X-ray1.75A/B/C1-171[»]
    3DR8X-ray1.95A/B1-171[»]
    ProteinModelPortaliQ8ZPD3.
    SMRiQ8ZPD3. Positions 2-163.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8ZPD3.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 163163N-acetyltransferasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni72 – 743Substrate bindingBy similarity
    Regioni82 – 843Substrate bindingBy similarity
    Regioni85 – 873Acetyl-CoA binding1 Publication
    Regioni93 – 986Acetyl-CoA binding1 Publication

    Sequence similaritiesi

    Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiENOG4108Z7F. Bacteria.
    COG1247. LUCA.
    HOGENOMiHOG000078516.
    KOiK03823.
    OMAiVTGQMPQ.
    PhylomeDBiQ8ZPD3.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8ZPD3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTIRFADKAD CAAITEIYNH AVLHTAAIWN DRTVDTDNRL AWYEARQLLG
    60 70 80 90 100
    YPVLVSEENG VVTGYASFGD WRSFDGFRYT VEHSVYVHPA HQGKGLGRKL
    110 120 130 140 150
    LSRLIDEARR CGKHVMVAGI ESQNAASIRL HHSLGFTVTA QMPQVGVKFG
    160 170
    RWLDLTFMQL QLDEHAAPDA C
    Length:171
    Mass (Da):19,188
    Last modified:March 1, 2002 - v1
    Checksum:iD40DD786C750E073
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL20508.1.
    RefSeqiNP_460549.1. NC_003197.1.
    WP_000155373.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20508; AAL20508; STM1590.
    GeneIDi1253108.
    KEGGistm:STM1590.
    PATRICi32381682. VBISalEnt20916_1681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL20508.1.
    RefSeqiNP_460549.1. NC_003197.1.
    WP_000155373.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DR6X-ray1.75A/B/C1-171[»]
    3DR8X-ray1.95A/B1-171[»]
    ProteinModelPortaliQ8ZPD3.
    SMRiQ8ZPD3. Positions 2-163.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi99287.STM1590.

    Proteomic databases

    PaxDbiQ8ZPD3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL20508; AAL20508; STM1590.
    GeneIDi1253108.
    KEGGistm:STM1590.
    PATRICi32381682. VBISalEnt20916_1681.

    Phylogenomic databases

    eggNOGiENOG4108Z7F. Bacteria.
    COG1247. LUCA.
    HOGENOMiHOG000078516.
    KOiK03823.
    OMAiVTGQMPQ.
    PhylomeDBiQ8ZPD3.

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-1600-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiQ8ZPD3.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMDDA_SALTY
    AccessioniPrimary (citable) accession number: Q8ZPD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 24, 2015
    Last sequence update: March 1, 2002
    Last modified: September 7, 2016
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.