Q8ZP51 (RLUB_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal large subunit pseudouridine synthase B
EC=5.4.99.22

Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B

Gene names

Name:	rluB
Ordered Locus Names:	STM1719

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA By similarity.
Catalytic activity	23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).
Sequence similarities	Belongs to the pseudouridine synthase RsuA family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Ribosomal large subunit pseudouridine synthase B		PRO_0000099990
Regions
	Domain	3 – 63	61	S4 RNA-binding
Sites
	Active site	110	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZP51 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 91EEC97F773F6EAF	FASTA	291	32,612
	10 20 30 40 50 60 MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIATLGD RVEVTPGLKI RIDGHLISVK 70 80 90 100 110 120 ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG ARWIAVGRLD VNTCGLLLFT 130 140 150 160 170 180 TDGELANRLM HPSREVEREY AVRVFGQVDE SKLRDLSRGV QLEDGPAAFK TIKFSGGEGI 190 200 210 220 230 240 NQWYNVTLTE GRNREVRRLW EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL 250 260 270 280 290 RGLVELPPET SSKVAVEKDR RRMKANQIRR AVKRHSQIAG GRRSGGRNNN G

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL20637.1.
RefSeq	NP_460678.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q8ZP51.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM1719.
Proteomic databases
PaxDb	Q8ZP51.
PRIDE	Q8ZP51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL20637; AAL20637; STM1719.
GeneID	1253238.
KEGG	stm:STM1719.
PATRIC	32381953. VBISalEnt20916_1816.
Phylogenomic databases
eggNOG	COG1187.
HOGENOM	HOG000044954.
KO	K06178.
OMA	PRGGWIE.
ProtClustDB	PRK10700.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR000748. PsdUridine_synth_RsuA/RluB/E/F. IPR018496. PsdUridine_synth_RsuA/RluB_CS. IPR006145. PsdUridine_synth_RsuA/RluD. IPR002942. S4_RNA-bd. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00093. TIGR00093. 1 hit.
PROSITE	PS01149. PSI_RSU. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLUB_SALTY

Accession

Primary (citable) accession number: Q8ZP51

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program