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Q8ZNY9

- PRP1_SALTY

UniProt

Q8ZNY9 - PRP1_SALTY

Protein

Serine/threonine-protein phosphatase 1

Gene

pphA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Can hydrolyze phosphorylated Ser-, Thr- or Tyr-substrates in vitro. The natural substrate is unknown.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Manganese.

    Enzyme regulationi

    Inhibited by cadmium, copper, zinc when added cobalt when added concomitantly with manganese.

    pH dependencei

    Optimum pH is 6-7.

    Temperature dependencei

    Optimum temperature is 45-55 degrees Celsius. Thermostable.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi24 – 241Manganese 1By similarity
    Metal bindingi26 – 261Manganese 1By similarity
    Metal bindingi53 – 531Manganese 1By similarity
    Metal bindingi53 – 531Manganese 2By similarity
    Metal bindingi79 – 791Manganese 2By similarity
    Active sitei80 – 801Proton donorBy similarity
    Metal bindingi185 – 1851Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-1861-MONOMER.

    Protein family/group databases

    PptaseDBiP3D0411148.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 1 (EC:3.1.3.16)
    Gene namesi
    Name:pphA
    Synonyms:prpA
    Ordered Locus Names:STM1853
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 216216Serine/threonine-protein phosphatase 1PRO_0000058909Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi99287.STM1853.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8ZNY9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000251869.
    KOiK07313.
    OMAiHDEFVIN.
    OrthoDBiEOG6KDKSC.
    PhylomeDBiQ8ZNY9.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8ZNY9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMRPEEIYQR IEAKNWRHVW VVGDIHGCFS MLMKRLRECR FDPQQDLLVS    50
    VGDLIDRGPD SLGCLALLRE SWMTAVRGNH EQMALDARAS SQSTLWLMNG 100
    GDWFTRLTAE HAAQAEALFI LCQRLPWILE VRCRHSTHVI AHADYPASTY 150
    QWQKKVDLHQ VLWSRERLIN KRGGISGADH FWFGHTPLRR RMDFANVHYI 200
    DTGAVFGGQL TLARIQ 216
    Length:216
    Mass (Da):25,037
    Last modified:March 1, 2002 - v1
    Checksum:iC2A62B823F4AAAD5
    GO

    Sequence cautioni

    The sequence AAL09831.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY049950 Genomic DNA. Translation: AAL09831.1. Different initiation.
    AE006468 Genomic DNA. Translation: AAL20768.1.
    RefSeqiNP_460809.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20768; AAL20768; STM1853.
    GeneIDi1253372.
    KEGGistm:STM1853.
    PATRICi32382235. VBISalEnt20916_1956.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY049950 Genomic DNA. Translation: AAL09831.1 . Different initiation.
    AE006468 Genomic DNA. Translation: AAL20768.1 .
    RefSeqi NP_460809.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali Q8ZNY9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM1853.

    Protein family/group databases

    PptaseDBi P3D0411148.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL20768 ; AAL20768 ; STM1853 .
    GeneIDi 1253372.
    KEGGi stm:STM1853.
    PATRICi 32382235. VBISalEnt20916_1956.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000251869.
    KOi K07313.
    OMAi HDEFVIN.
    OrthoDBi EOG6KDKSC.
    PhylomeDBi Q8ZNY9.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-1861-MONOMER.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties."
      Shi L., Kehres D.G., Maguire M.E.
      J. Bacteriol. 183:7053-7057(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.

    Entry informationi

    Entry nameiPRP1_SALTY
    AccessioniPrimary (citable) accession number: Q8ZNY9
    Secondary accession number(s): Q8VPE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Neither magnesium nor calcium stimulates activity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3