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Protein

Serine/threonine-protein phosphatase 1

Gene

pphA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Can hydrolyze phosphorylated Ser-, Thr- or Tyr-substrates in vitro. The natural substrate is unknown.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Enzyme regulationi

Inhibited by cadmium, copper, zinc when added cobalt when added concomitantly with manganese.

pH dependencei

Optimum pH is 6-7.

Temperature dependencei

Optimum temperature is 45-55 degrees Celsius. Thermostable.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Manganese 1By similarity
Metal bindingi26 – 261Manganese 1By similarity
Metal bindingi53 – 531Manganese 1By similarity
Metal bindingi53 – 531Manganese 2By similarity
Metal bindingi79 – 791Manganese 2By similarity
Active sitei80 – 801Proton donorBy similarity
Metal bindingi185 – 1851Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1861-MONOMER.

Protein family/group databases

PptaseDBiP3D0411148.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 1 (EC:3.1.3.16)
Gene namesi
Name:pphA
Synonyms:prpA
Ordered Locus Names:STM1853
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Serine/threonine-protein phosphatase 1PRO_0000058909Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi99287.STM1853.

Structurei

3D structure databases

ProteinModelPortaliQ8ZNY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000251869.
KOiK07313.
OMAiMNGGDWY.
OrthoDBiEOG6KDKSC.
PhylomeDBiQ8ZNY9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZNY9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMRPEEIYQR IEAKNWRHVW VVGDIHGCFS MLMKRLRECR FDPQQDLLVS
60 70 80 90 100
VGDLIDRGPD SLGCLALLRE SWMTAVRGNH EQMALDARAS SQSTLWLMNG
110 120 130 140 150
GDWFTRLTAE HAAQAEALFI LCQRLPWILE VRCRHSTHVI AHADYPASTY
160 170 180 190 200
QWQKKVDLHQ VLWSRERLIN KRGGISGADH FWFGHTPLRR RMDFANVHYI
210
DTGAVFGGQL TLARIQ
Length:216
Mass (Da):25,037
Last modified:March 1, 2002 - v1
Checksum:iC2A62B823F4AAAD5
GO

Sequence cautioni

The sequence AAL09831.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049950 Genomic DNA. Translation: AAL09831.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL20768.1.
RefSeqiNP_460809.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20768; AAL20768; STM1853.
GeneIDi1253372.
KEGGistm:STM1853.
PATRICi32382235. VBISalEnt20916_1956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049950 Genomic DNA. Translation: AAL09831.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL20768.1.
RefSeqiNP_460809.1. NC_003197.1.

3D structure databases

ProteinModelPortaliQ8ZNY9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1853.

Protein family/group databases

PptaseDBiP3D0411148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20768; AAL20768; STM1853.
GeneIDi1253372.
KEGGistm:STM1853.
PATRICi32382235. VBISalEnt20916_1956.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000251869.
KOiK07313.
OMAiMNGGDWY.
OrthoDBiEOG6KDKSC.
PhylomeDBiQ8ZNY9.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1861-MONOMER.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties."
    Shi L., Kehres D.G., Maguire M.E.
    J. Bacteriol. 183:7053-7057(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiPRP1_SALTY
AccessioniPrimary (citable) accession number: Q8ZNY9
Secondary accession number(s): Q8VPE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Neither magnesium nor calcium stimulates activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.