Q8ZNY9 (PRP1_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 1
EC=3.1.3.16

Gene names

Name:	pphA
Synonyms:	prpA
Ordered Locus Names:	STM1853

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can hydrolyze phosphorylated Ser-, Thr- or Tyr-substrates in vitro. The natural substrate is unknown.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Manganese.
Enzyme regulation	Inhibited by cadmium, copper, zinc when added concomitantly with manganese. Slightly inhibited by iron and cobalt when added concomitantly with manganese.
Miscellaneous	Neither magnesium nor calcium stimulates activity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.
Biophysicochemical properties	pH dependence: Optimum pH is 6-7. Temperature dependence: Optimum temperature is 45-55 degrees Celsius. Thermostable.
Sequence caution	The sequence AAL09831.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Manganese
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Serine/threonine-protein phosphatase 1		PRO_0000058909
Sites
	Active site	80	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZNY9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C2A62B823F4AAAD5	FASTA	216	25,037
	10 20 30 40 50 60 MMRPEEIYQR IEAKNWRHVW VVGDIHGCFS MLMKRLRECR FDPQQDLLVS VGDLIDRGPD 70 80 90 100 110 120 SLGCLALLRE SWMTAVRGNH EQMALDARAS SQSTLWLMNG GDWFTRLTAE HAAQAEALFI 130 140 150 160 170 180 LCQRLPWILE VRCRHSTHVI AHADYPASTY QWQKKVDLHQ VLWSRERLIN KRGGISGADH 190 200 210 FWFGHTPLRR RMDFANVHYI DTGAVFGGQL TLARIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties." Shi L., Kehres D.G., Maguire M.E. J. Bacteriol. 183:7053-7057(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY049950 Genomic DNA. Translation: AAL09831.1. Different initiation. AE006468 Genomic DNA. Translation: AAL20768.1.
RefSeq	NP_460809.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q8ZNY9.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM1853.
Protein family/group databases
PptaseDB	P3D0411148.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL20768; AAL20768; STM1853.
GeneID	1253372.
KEGG	stm:STM1853.
PATRIC	32382235. VBISalEnt20916_1956.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000251869.
KO	K07313.
OMA	MMKQPAP.
ProtClustDB	PRK11439.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRP1_SALTY

Accession

Primary (citable) accession number: Q8ZNY9
Secondary accession number(s): Q8VPE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program