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Q8ZNY9 (PRP1_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 1

EC=3.1.3.16
Gene names
Name:pphA
Synonyms:prpA
Ordered Locus Names:STM1853
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can hydrolyze phosphorylated Ser-, Thr- or Tyr-substrates in vitro. The natural substrate is unknown.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Manganese.

Enzyme regulation

Inhibited by cadmium, copper, zinc when added cobalt when added concomitantly with manganese.

Miscellaneous

Neither magnesium nor calcium stimulates activity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 6-7.

Temperature dependence:

Optimum temperature is 45-55 degrees Celsius. Thermostable.

Sequence caution

The sequence AAL09831.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 216216Serine/threonine-protein phosphatase 1
PRO_0000058909

Sites

Active site801Proton donor By similarity
Metal binding241Manganese 1 By similarity
Metal binding261Manganese 1 By similarity
Metal binding531Manganese 1 By similarity
Metal binding531Manganese 2 By similarity
Metal binding791Manganese 2 By similarity
Metal binding1851Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ZNY9 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C2A62B823F4AAAD5

FASTA21625,037
        10         20         30         40         50         60 
MMRPEEIYQR IEAKNWRHVW VVGDIHGCFS MLMKRLRECR FDPQQDLLVS VGDLIDRGPD 

        70         80         90        100        110        120 
SLGCLALLRE SWMTAVRGNH EQMALDARAS SQSTLWLMNG GDWFTRLTAE HAAQAEALFI 

       130        140        150        160        170        180 
LCQRLPWILE VRCRHSTHVI AHADYPASTY QWQKKVDLHQ VLWSRERLIN KRGGISGADH 

       190        200        210 
FWFGHTPLRR RMDFANVHYI DTGAVFGGQL TLARIQ 

« Hide

References

« Hide 'large scale' references
[1]"The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties."
Shi L., Kehres D.G., Maguire M.E.
J. Bacteriol. 183:7053-7057(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY049950 Genomic DNA. Translation: AAL09831.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL20768.1.
RefSeqNP_460809.1. NC_003197.1.

3D structure databases

ProteinModelPortalQ8ZNY9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM1853.

Protein family/group databases

PptaseDBP3D0411148.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL20768; AAL20768; STM1853.
GeneID1253372.
KEGGstm:STM1853.
PATRIC32382235. VBISalEnt20916_1956.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000251869.
KOK07313.
OMAHDEFVIN.
OrthoDBEOG6KDKSC.
PhylomeDBQ8ZNY9.

Enzyme and pathway databases

BioCycSENT99287:GCTI-1861-MONOMER.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePRP1_SALTY
AccessionPrimary (citable) accession number: Q8ZNY9
Secondary accession number(s): Q8VPE2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families