Reviewed, UniProtKB/Swiss-Prot Q8ZNW0 (KPYK2_SALTY)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase II
    EC=2.7.1.40
Alternative name(s):
    PK-2

Gene names

Name:	pykA
Ordered Locus Names:	STM1888

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Enzyme regulation	Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 480	479	Pyruvate kinase II		PRO_0000112074
Sites
	Active site	223	1	By similarity
	Metal binding	225	1	Magnesium By similarity
	Metal binding	249	1	Magnesium By similarity
	Metal binding	250	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZNW0-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8BF3948FE958EFAC	FASTA	480	51,387
	10 20 30 40 50 60 MSRRLRRTKI VTTLGPATDR DNNLEKVIAA GANVVRMNFS HGSPEDHKMR ADKVREIAAK 70 80 90 100 110 120 LGRHVAILGD LQGPKIRVST FKEGKVFLNI GDKFLLDANL GKGEGDKEKV GIDYKGLPAD 130 140 150 160 170 180 VVPGDILLLD DGRVQLKVLE VQGMKVFTEV TVGGPLSNNK GINKLGGGLS AEALTEKDKA 190 200 210 220 230 240 DIQTAALIGV DYLAVSFPRC GEDLNYARRL ARDAGCDAKI VAKVERAEAV CDQNAMDDII 250 260 270 280 290 300 LASDVVMVAR GDLGVEIGDP ELVGIQKALI RRARQLNRAV ITATQMMESM ITNPMPTRAE 310 320 330 340 350 360 VMDVANAVLD GTDAVMLSAE TAAGQYPSET VAAMARVCLG AEKIPSINVS KHRLDVQFDN 370 380 390 400 410 420 VEEAIAMSAM YAANHLKGVT AIITMTESGR TALMTSRISS GLPIFAMSRH ERTLNLTALY 430 440 450 460 470 480 RGVTPVHFDS AADGVVAAHE AVNLLRDKGY LVSGDLVIVT QGDVMSTVGS TNTTRILTVE

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008784 Genomic DNA. Translation: AAL20804.1.
RefSeq	NP_460845.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Genome annotation databases
GeneID	1253409.
GenomeReviews	Gene locus STM1888 in contig AE006468_GR.
KEGG	stm:STM1888.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZNW0.
OMA	Q8ZNW0. RMIKLAR.
Enzyme and pathway databases
BioCyc	STYP99287:STM1888-MON.
BRENDA	2.7.1.40. 2.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK2_SALTY

Accession

Primary (citable) accession number: Q8ZNW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 42 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)