Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase II

Gene

pykA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Enzyme regulationi

Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK (By similarity).By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), Probable phosphoglycerate mutase GpmB (gpmB), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase I (pykF), Pyruvate kinase II (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Metal bindingi38PotassiumBy similarity1
Metal bindingi40PotassiumBy similarity1
Metal bindingi70PotassiumBy similarity1
Sitei223Transition state stabilizerBy similarity1
Metal bindingi225MagnesiumBy similarity1
Binding sitei251Substrate; via amide nitrogenBy similarity1
Metal bindingi252MagnesiumBy similarity1
Binding sitei252Substrate; via amide nitrogenBy similarity1
Binding sitei284SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyruvate

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-1907-MONOMER
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase II (EC:2.7.1.40)
Alternative name(s):
PK-2
Gene namesi
Name:pykA
Ordered Locus Names:STM1888
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001120742 – 480Pyruvate kinase IIAdd BLAST479

Proteomic databases

PaxDbiQ8ZNW0
PRIDEiQ8ZNW0

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi99287.STM1888

Structurei

3D structure databases

ProteinModelPortaliQ8ZNW0
SMRiQ8ZNW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
HOGENOMiHOG000021558
KOiK00873
OMAiICVEAEK
PhylomeDBiQ8ZNW0

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8ZNW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRLRRTKI VTTLGPATDR DNNLEKVIAA GANVVRMNFS HGSPEDHKMR
60 70 80 90 100
ADKVREIAAK LGRHVAILGD LQGPKIRVST FKEGKVFLNI GDKFLLDANL
110 120 130 140 150
GKGEGDKEKV GIDYKGLPAD VVPGDILLLD DGRVQLKVLE VQGMKVFTEV
160 170 180 190 200
TVGGPLSNNK GINKLGGGLS AEALTEKDKA DIQTAALIGV DYLAVSFPRC
210 220 230 240 250
GEDLNYARRL ARDAGCDAKI VAKVERAEAV CDQNAMDDII LASDVVMVAR
260 270 280 290 300
GDLGVEIGDP ELVGIQKALI RRARQLNRAV ITATQMMESM ITNPMPTRAE
310 320 330 340 350
VMDVANAVLD GTDAVMLSAE TAAGQYPSET VAAMARVCLG AEKIPSINVS
360 370 380 390 400
KHRLDVQFDN VEEAIAMSAM YAANHLKGVT AIITMTESGR TALMTSRISS
410 420 430 440 450
GLPIFAMSRH ERTLNLTALY RGVTPVHFDS AADGVVAAHE AVNLLRDKGY
460 470 480
LVSGDLVIVT QGDVMSTVGS TNTTRILTVE
Length:480
Mass (Da):51,387
Last modified:January 23, 2007 - v3
Checksum:i8BF3948FE958EFAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA Translation: AAL20804.1
RefSeqiNP_460845.1, NC_003197.2
WP_000091156.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL20804; AAL20804; STM1888
GeneIDi1253409
KEGGistm:STM1888
PATRICifig|99287.12.peg.2002

Similar proteinsi

Entry informationi

Entry nameiKPYK2_SALTY
AccessioniPrimary (citable) accession number: Q8ZNW0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 96 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health