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Protein

Pyruvate kinase II

Gene

pykA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateBy similarity
Metal bindingi38 – 381PotassiumBy similarity
Metal bindingi40 – 401PotassiumBy similarity
Metal bindingi70 – 701PotassiumBy similarity
Sitei223 – 2231Transition state stabilizerBy similarity
Metal bindingi225 – 2251MagnesiumBy similarity
Binding sitei251 – 2511Substrate; via amide nitrogenBy similarity
Metal bindingi252 – 2521MagnesiumBy similarity
Binding sitei252 – 2521Substrate; via amide nitrogenBy similarity
Binding sitei284 – 2841SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyruvate

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1900-MONOMER.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase II (EC:2.7.1.40)
Alternative name(s):
PK-2
Gene namesi
Name:pykA
Ordered Locus Names:STM1888
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 480479Pyruvate kinase IIPRO_0000112074Add
BLAST

Proteomic databases

PaxDbiQ8ZNW0.
PRIDEiQ8ZNW0.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi99287.STM1888.

Structurei

3D structure databases

ProteinModelPortaliQ8ZNW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
KOiK00873.
OMAiTPVYFDS.
OrthoDBiEOG6GBMB0.
PhylomeDBiQ8ZNW0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8ZNW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRLRRTKI VTTLGPATDR DNNLEKVIAA GANVVRMNFS HGSPEDHKMR
60 70 80 90 100
ADKVREIAAK LGRHVAILGD LQGPKIRVST FKEGKVFLNI GDKFLLDANL
110 120 130 140 150
GKGEGDKEKV GIDYKGLPAD VVPGDILLLD DGRVQLKVLE VQGMKVFTEV
160 170 180 190 200
TVGGPLSNNK GINKLGGGLS AEALTEKDKA DIQTAALIGV DYLAVSFPRC
210 220 230 240 250
GEDLNYARRL ARDAGCDAKI VAKVERAEAV CDQNAMDDII LASDVVMVAR
260 270 280 290 300
GDLGVEIGDP ELVGIQKALI RRARQLNRAV ITATQMMESM ITNPMPTRAE
310 320 330 340 350
VMDVANAVLD GTDAVMLSAE TAAGQYPSET VAAMARVCLG AEKIPSINVS
360 370 380 390 400
KHRLDVQFDN VEEAIAMSAM YAANHLKGVT AIITMTESGR TALMTSRISS
410 420 430 440 450
GLPIFAMSRH ERTLNLTALY RGVTPVHFDS AADGVVAAHE AVNLLRDKGY
460 470 480
LVSGDLVIVT QGDVMSTVGS TNTTRILTVE
Length:480
Mass (Da):51,387
Last modified:January 23, 2007 - v3
Checksum:i8BF3948FE958EFAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20804.1.
RefSeqiNP_460845.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20804; AAL20804; STM1888.
GeneIDi1253409.
KEGGistm:STM1888.
PATRICi32382327. VBISalEnt20916_2002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20804.1.
RefSeqiNP_460845.1. NC_003197.1.

3D structure databases

ProteinModelPortaliQ8ZNW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1888.

Proteomic databases

PaxDbiQ8ZNW0.
PRIDEiQ8ZNW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20804; AAL20804; STM1888.
GeneIDi1253409.
KEGGistm:STM1888.
PATRICi32382327. VBISalEnt20916_2002.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
KOiK00873.
OMAiTPVYFDS.
OrthoDBiEOG6GBMB0.
PhylomeDBiQ8ZNW0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.
BioCyciSENT99287:GCTI-1900-MONOMER.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiKPYK2_SALTY
AccessioniPrimary (citable) accession number: Q8ZNW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.