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Protein

Probable lipid kinase YegS

Gene

yegS

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In vitro does not phosphorylate diacylglycerol, sphingosine, N,N-dimethylsphingosine; threo-dihydrosphingosine, erythro-dihydrosphingosine, C2 ceramide, C6 ceramide, phosphatidylinositol or dimethylsphingosine. The potential in vivo substrate is unknown.1 Publication

Cofactori

Mg2+Curated1 Publication, Ca2+Curated1 PublicationNote: Binds 1 Mg2+ ion per subunit. Ca2+ may be able to substitute. 2 Ca2+ ions are seen in the crystal structure; one of the two occupies the same site as Mg2+ in other family members.Curated1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATPBy similarity
Binding sitei95 – 951ATPBy similarity
Metal bindingi125 – 1251Calcium 1
Metal bindingi200 – 2001Calcium 1
Metal bindingi215 – 2151Calcium 1; via carbonyl oxygen
Metal bindingi218 – 2181Calcium 1
Metal bindingi220 – 2201Calcium 1; via carbonyl oxygen
Metal bindingi242 – 2421Calcium 2; via carbonyl oxygen
Metal bindingi243 – 2431Calcium 2; via carbonyl oxygen
Metal bindingi245 – 2451Calcium 2; via carbonyl oxygen
Metal bindingi248 – 2481Calcium 2; via carbonyl oxygen
Active sitei271 – 2711Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 727ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2153-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lipid kinase YegS (EC:2.7.1.-)
Gene namesi
Name:yegS
Ordered Locus Names:STM2140
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Probable lipid kinase YegSPRO_0000292157Add
BLAST

Proteomic databases

PaxDbiQ8ZNP1.
PRIDEiQ8ZNP1.

Interactioni

Subunit structurei

Homodimer. May interact with the periplasmic chaperone Skp (also known as OmpH); this suggests the protein may be translocated to the periplasm and then sorted to the outer membrane.1 Publication

Protein-protein interaction databases

STRINGi99287.STM2140.

Structurei

Secondary structure

1
299
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Helixi13 – 153Combined sources
Helixi19 – 3012Combined sources
Beta strandi35 – 406Combined sources
Helixi45 – 5511Combined sources
Beta strandi59 – 668Combined sources
Helixi67 – 7711Combined sources
Beta strandi87 – 904Combined sources
Helixi98 – 1036Combined sources
Helixi109 – 11810Combined sources
Beta strandi121 – 1244Combined sources
Beta strandi127 – 1293Combined sources
Turni130 – 1323Combined sources
Beta strandi133 – 14210Combined sources
Beta strandi144 – 1463Combined sources
Helixi161 – 1677Combined sources
Beta strandi172 – 1754Combined sources
Beta strandi178 – 1847Combined sources
Beta strandi187 – 20418Combined sources
Turni205 – 2073Combined sources
Beta strandi208 – 2114Combined sources
Beta strandi221 – 2266Combined sources
Helixi232 – 2354Combined sources
Helixi239 – 2446Combined sources
Beta strandi248 – 26821Combined sources
Beta strandi271 – 28212Combined sources
Beta strandi287 – 2904Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P1RX-ray2.50A/B/C/D1-299[»]
ProteinModelPortaliQ8ZNP1.
SMRiQ8ZNP1. Positions 1-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZNP1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 133132DAGKcAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.Curated

Phylogenomic databases

eggNOGiENOG4107TE5. Bacteria.
COG1597. LUCA.
OMAiHWQGDLL.
OrthoDBiEOG613098.
PhylomeDBiQ8ZNP1.

Family and domain databases

HAMAPiMF_01377. YegS.
InterProiIPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR022433. Lip_kinase_YegS.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR03702. lip_kinase_YegS. 1 hit.
TIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZNP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFPASLLI LNGKSADNQP LREAITLLRD EGIQIHVRVT WEKGDAQRYV
60 70 80 90 100
DEARRLGVET VIAGGGDGTI NEVSTALIQI RDGVAPALGL LPLGTANDFA
110 120 130 140 150
TSAGIPEALD KALKLAIAGN AMEIDMAMVN DKTCFINMAT GGFGTRITTE
160 170 180 190 200
TPEKLKAALG GVSYLIHGLM RMDTLTPDRC EIRGENFHWQ GDALVIGIGN
210 220 230 240 250
GRQAGGGQQL CPTALINDGL LQLRIFTGEE LLPALFSTLT QSDDNPNIID
260 270 280 290
GASAWFDIHA PHEITFNLDG EPLSGQEFHI EVLPGALRCR LPPDCPLLR
Length:299
Mass (Da):32,029
Last modified:March 1, 2002 - v1
Checksum:i4026FEFD5F2F0197
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL21043.1.
RefSeqiNP_461084.1. NC_003197.1.
WP_001273389.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21043; AAL21043; STM2140.
GeneIDi1253661.
KEGGistm:STM2140.
PATRICi32382869. VBISalEnt20916_2265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL21043.1.
RefSeqiNP_461084.1. NC_003197.1.
WP_001273389.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P1RX-ray2.50A/B/C/D1-299[»]
ProteinModelPortaliQ8ZNP1.
SMRiQ8ZNP1. Positions 1-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2140.

Proteomic databases

PaxDbiQ8ZNP1.
PRIDEiQ8ZNP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL21043; AAL21043; STM2140.
GeneIDi1253661.
KEGGistm:STM2140.
PATRICi32382869. VBISalEnt20916_2265.

Phylogenomic databases

eggNOGiENOG4107TE5. Bacteria.
COG1597. LUCA.
OMAiHWQGDLL.
OrthoDBiEOG613098.
PhylomeDBiQ8ZNP1.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2153-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8ZNP1.

Family and domain databases

HAMAPiMF_01377. YegS.
InterProiIPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR022433. Lip_kinase_YegS.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR03702. lip_kinase_YegS. 1 hit.
TIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Characterization of Salmonella typhimurium yegS, a putative lipid kinase homologous to eukaryotic sphingosine and diacylglycerol kinases."
    Nichols C.E., Lamb H.K., Lockyer M., Charles I.G., Pyne S., Hawkins A.R., Stammers D.K.
    Proteins 68:13-25(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS), FUNCTION, COFACTOR, SUBUNIT, POSSIBLE INTERACTION WITH SKP.
    Strain: SL3261.

Entry informationi

Entry nameiYEGS_SALTY
AccessioniPrimary (citable) accession number: Q8ZNP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: March 1, 2002
Last modified: November 11, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.