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Protein

Probable lipid kinase YegS

Gene

yegS

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In vitro does not phosphorylate diacylglycerol, sphingosine, N,N-dimethylsphingosine; threo-dihydrosphingosine, erythro-dihydrosphingosine, C2 ceramide, C6 ceramide, phosphatidylinositol or dimethylsphingosine. The potential in vivo substrate is unknown.1 Publication

Cofactori

Mg2+Curated1 Publication, Ca2+Curated1 PublicationNote: Binds 1 Mg2+ ion per subunit. Ca2+ may be able to substitute. 2 Ca2+ ions are seen in the crystal structure; one of the two occupies the same site as Mg2+ in other family members.Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPBy similarity1
Binding sitei95ATPBy similarity1
Metal bindingi125Calcium 11
Metal bindingi200Calcium 11
Metal bindingi215Calcium 1; via carbonyl oxygen1
Metal bindingi218Calcium 11
Metal bindingi220Calcium 1; via carbonyl oxygen1
Metal bindingi242Calcium 2; via carbonyl oxygen1
Metal bindingi243Calcium 2; via carbonyl oxygen1
Metal bindingi245Calcium 2; via carbonyl oxygen1
Metal bindingi248Calcium 2; via carbonyl oxygen1
Active sitei271Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 72ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lipid kinase YegS (EC:2.7.1.-)
Gene namesi
Name:yegS
Ordered Locus Names:STM2140
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002921571 – 299Probable lipid kinase YegSAdd BLAST299

Proteomic databases

PaxDbiQ8ZNP1.
PRIDEiQ8ZNP1.

Interactioni

Subunit structurei

Homodimer. May interact with the periplasmic chaperone Skp (also known as OmpH); this suggests the protein may be translocated to the periplasm and then sorted to the outer membrane.1 Publication

Protein-protein interaction databases

STRINGi99287.STM2140.

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Helixi13 – 15Combined sources3
Helixi19 – 30Combined sources12
Beta strandi35 – 40Combined sources6
Helixi45 – 55Combined sources11
Beta strandi59 – 66Combined sources8
Helixi67 – 77Combined sources11
Beta strandi87 – 90Combined sources4
Helixi98 – 103Combined sources6
Helixi109 – 118Combined sources10
Beta strandi121 – 124Combined sources4
Beta strandi127 – 129Combined sources3
Turni130 – 132Combined sources3
Beta strandi133 – 142Combined sources10
Beta strandi144 – 146Combined sources3
Helixi161 – 167Combined sources7
Beta strandi172 – 175Combined sources4
Beta strandi178 – 184Combined sources7
Beta strandi187 – 204Combined sources18
Turni205 – 207Combined sources3
Beta strandi208 – 211Combined sources4
Beta strandi221 – 226Combined sources6
Helixi232 – 235Combined sources4
Helixi239 – 244Combined sources6
Beta strandi248 – 268Combined sources21
Beta strandi271 – 282Combined sources12
Beta strandi287 – 290Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P1RX-ray2.50A/B/C/D1-299[»]
ProteinModelPortaliQ8ZNP1.
SMRiQ8ZNP1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZNP1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 133DAGKcAdd BLAST132

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107TE5. Bacteria.
COG1597. LUCA.
OMAiHWQGDLL.
PhylomeDBiQ8ZNP1.

Family and domain databases

HAMAPiMF_01377. YegS. 1 hit.
InterProiView protein in InterPro
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR022433. Lip_kinase_YegS.
IPR016064. NAD/diacylglycerol_kinase.
PfamiView protein in Pfam
PF00781. DAGK_cat. 1 hit.
SMARTiView protein in SMART
SM00046. DAGKc. 1 hit.
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR03702. lip_kinase_YegS. 1 hit.
TIGR00147. TIGR00147. 1 hit.
PROSITEiView protein in PROSITE
PS50146. DAGK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZNP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFPASLLI LNGKSADNQP LREAITLLRD EGIQIHVRVT WEKGDAQRYV
60 70 80 90 100
DEARRLGVET VIAGGGDGTI NEVSTALIQI RDGVAPALGL LPLGTANDFA
110 120 130 140 150
TSAGIPEALD KALKLAIAGN AMEIDMAMVN DKTCFINMAT GGFGTRITTE
160 170 180 190 200
TPEKLKAALG GVSYLIHGLM RMDTLTPDRC EIRGENFHWQ GDALVIGIGN
210 220 230 240 250
GRQAGGGQQL CPTALINDGL LQLRIFTGEE LLPALFSTLT QSDDNPNIID
260 270 280 290
GASAWFDIHA PHEITFNLDG EPLSGQEFHI EVLPGALRCR LPPDCPLLR
Length:299
Mass (Da):32,029
Last modified:March 1, 2002 - v1
Checksum:i4026FEFD5F2F0197
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL21043.1.
RefSeqiNP_461084.1. NC_003197.2.
WP_001273389.1. NC_003197.2.

Genome annotation databases

EnsemblBacteriaiAAL21043; AAL21043; STM2140.
GeneIDi1253661.
KEGGistm:STM2140.
PATRICi32382869. VBISalEnt20916_2265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL21043.1.
RefSeqiNP_461084.1. NC_003197.2.
WP_001273389.1. NC_003197.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P1RX-ray2.50A/B/C/D1-299[»]
ProteinModelPortaliQ8ZNP1.
SMRiQ8ZNP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2140.

Proteomic databases

PaxDbiQ8ZNP1.
PRIDEiQ8ZNP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL21043; AAL21043; STM2140.
GeneIDi1253661.
KEGGistm:STM2140.
PATRICi32382869. VBISalEnt20916_2265.

Phylogenomic databases

eggNOGiENOG4107TE5. Bacteria.
COG1597. LUCA.
OMAiHWQGDLL.
PhylomeDBiQ8ZNP1.

Miscellaneous databases

EvolutionaryTraceiQ8ZNP1.

Family and domain databases

HAMAPiMF_01377. YegS. 1 hit.
InterProiView protein in InterPro
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR022433. Lip_kinase_YegS.
IPR016064. NAD/diacylglycerol_kinase.
PfamiView protein in Pfam
PF00781. DAGK_cat. 1 hit.
SMARTiView protein in SMART
SM00046. DAGKc. 1 hit.
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR03702. lip_kinase_YegS. 1 hit.
TIGR00147. TIGR00147. 1 hit.
PROSITEiView protein in PROSITE
PS50146. DAGK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYEGS_SALTY
AccessioniPrimary (citable) accession number: Q8ZNP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: March 1, 2002
Last modified: March 15, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.