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Protein

UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase

Gene

arnB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) (PubMed:12429098, PubMed:24460375). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity).By similarity2 Publications

Catalytic activityi

UDP-4-amino-4-deoxy-beta-L-arabinose + 2-oxoglutarate = UDP-beta-L-threo-pentapyranos-4-ulose + L-glutamate.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Enzyme regulationi

Inhibited by L-cycloserine.1 Publication

Pathwayi: UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Bifunctional polymyxin resistance protein ArnA (arnA)
  2. UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB)
  3. Bifunctional polymyxin resistance protein ArnA (arnA)
This subpathway is part of the pathway UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate, the pathway UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei188Proton acceptor2 Publications1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00032; UER00493.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (EC:2.6.1.871 Publication)
Alternative name(s):
Polymyxin resistance protein PmrH
UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase
Short name:
UDP-Ara4O aminotransferase
UDP-4-amino-4-deoxy-L-arabinose aminotransferase
Gene namesi
Name:arnB
Synonyms:pbgP, pbgP1, pmrH
Ordered Locus Names:STM2297
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi188K → A: Loss of covalent pyridoxal phosphate binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001100281 – 385UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferaseAdd BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188N6-(pyridoxal phosphate)lysineCombined sources1 Publication1

Proteomic databases

PaxDbiQ8ZNF3.
PRIDEiQ8ZNF3.

Expressioni

Inductioni

Induced by BasR.1 Publication

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi99287.STM2297.

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 32Combined sources13
Beta strandi36 – 38Combined sources3
Helixi39 – 52Combined sources14
Beta strandi55 – 61Combined sources7
Helixi63 – 73Combined sources11
Beta strandi81 – 88Combined sources8
Helixi90 – 98Combined sources9
Beta strandi102 – 106Combined sources5
Turni110 – 112Combined sources3
Helixi117 – 123Combined sources7
Beta strandi128 – 131Combined sources4
Helixi136 – 138Combined sources3
Helixi143 – 153Combined sources11
Beta strandi157 – 160Combined sources4
Beta strandi176 – 183Combined sources8
Beta strandi188 – 190Combined sources3
Beta strandi192 – 194Combined sources3
Beta strandi196 – 201Combined sources6
Helixi203 – 212Combined sources10
Beta strandi234 – 237Combined sources4
Helixi246 – 257Combined sources12
Helixi259 – 278Combined sources20
Beta strandi299 – 302Combined sources4
Helixi305 – 308Combined sources4
Helixi312 – 321Combined sources10
Helixi333 – 335Combined sources3
Helixi337 – 342Combined sources6
Helixi349 – 355Combined sources7
Beta strandi358 – 361Combined sources4
Helixi369 – 383Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MDOX-ray1.70A1-385[»]
1MDXX-ray1.96A1-385[»]
1MDZX-ray2.07A1-385[»]
4OCAX-ray2.30A1-385[»]
ProteinModelPortaliQ8ZNF3.
SMRiQ8ZNF3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZNF3.

Family & Domainsi

Sequence similaritiesi

Belongs to the DegT/DnrJ/EryC1 family. ArnB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CF4. Bacteria.
COG0399. LUCA.
HOGENOMiHOG000230163.
KOiK07806.
OMAiSEWNSAR.
PhylomeDBiQ8ZNF3.

Family and domain databases

CDDicd00616. AHBA_syn. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01167. ArnB_transfer. 1 hit.
InterProiIPR022850. ArnB_NH2Trfase.
IPR000653. DegT/StrS_aminotransferase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
[Graphical view]
PIRSFiPIRSF000390. PLP_StrS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZNF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGKMMSDF LPFSRPAMGA EELAAVKTVL DSGWITTGPK NQELEAAFCR
60 70 80 90 100
LTGNQYAVAV SSATAGMHIA LMALGIGEGD EVITPSMTWV STLNMIVLLG
110 120 130 140 150
ANPVMVDVDR DTLMVTPEHI EAAITPQTKA IIPVHYAGAP ADLDAIYALG
160 170 180 190 200
ERYGIPVIED AAHATGTSYK GRHIGARGTA IFSFHAIKNI TCAEGGIVVT
210 220 230 240 250
DNPQFADKLR SLKFHGLGVD AWDRQSGGRA PQAEVLAPGY KYNLPDLNAA
260 270 280 290 300
IALAQLQKLD ALNARRAAIA AQYHQAMADL PFQPLSLPSW EHIHAWHLFI
310 320 330 340 350
IRVDEARCGI TRDALMASLK TKGIGTGLHF RAAHTQKYYR ERFPTLTLPD
360 370 380
TEWNSERICS LPLFPDMTES DFDRVITALH QIAGQ
Length:385
Mass (Da):41,813
Last modified:November 30, 2016 - v3
Checksum:iDAA6C24FC591FA05
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti285L → V in AAC04770 (PubMed:11677609).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036677 Genomic DNA. Translation: AAC04770.1.
AE006468 Genomic DNA. Translation: AAL21198.1.
RefSeqiNP_461239.2. NC_003197.1.
WP_001279284.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21198; AAL21198; STM2297.
GeneIDi1253819.
KEGGistm:STM2297.
PATRICi32383205. VBISalEnt20916_2432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036677 Genomic DNA. Translation: AAC04770.1.
AE006468 Genomic DNA. Translation: AAL21198.1.
RefSeqiNP_461239.2. NC_003197.1.
WP_001279284.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MDOX-ray1.70A1-385[»]
1MDXX-ray1.96A1-385[»]
1MDZX-ray2.07A1-385[»]
4OCAX-ray2.30A1-385[»]
ProteinModelPortaliQ8ZNF3.
SMRiQ8ZNF3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2297.

Proteomic databases

PaxDbiQ8ZNF3.
PRIDEiQ8ZNF3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL21198; AAL21198; STM2297.
GeneIDi1253819.
KEGGistm:STM2297.
PATRICi32383205. VBISalEnt20916_2432.

Phylogenomic databases

eggNOGiENOG4105CF4. Bacteria.
COG0399. LUCA.
HOGENOMiHOG000230163.
KOiK07806.
OMAiSEWNSAR.
PhylomeDBiQ8ZNF3.

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00032; UER00493.

Miscellaneous databases

EvolutionaryTraceiQ8ZNF3.

Family and domain databases

CDDicd00616. AHBA_syn. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01167. ArnB_transfer. 1 hit.
InterProiIPR022850. ArnB_NH2Trfase.
IPR000653. DegT/StrS_aminotransferase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
[Graphical view]
PIRSFiPIRSF000390. PLP_StrS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARNB_SALTY
AccessioniPrimary (citable) accession number: Q8ZNF3
Secondary accession number(s): O52323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: November 30, 2016
Last modified: November 30, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.