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Q8ZND6

- PTA_SALTY

UniProt

Q8ZND6 - PTA_SALTY

Protein

Phosphate acetyltransferase

Gene

pta

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Involved in acetate metabolism. Catalyzes the reversible interconversion of acetyl-CoA and acetyl phosphate. The direction of the overall reaction changes depending on growth conditions. Required for acetate recapture but not for acetate excretion when this organism is grown on ethanolamine.1 Publication

    Catalytic activityi

    Acetyl-CoA + phosphate = CoA + acetyl phosphate.

    Enzyme regulationi

    Allosterically inhibited by NADH.1 Publication

    Kineticsi

    1. KM=162.1 µM for acetyl-CoA1 Publication
    2. KM=329.3 µM for acetyl phosphate1 Publication

    Vmax=142.2 µM/h/mg enzyme for acetyl-CoA-forming reaction1 Publication

    Vmax=20.6 µM/h/mg enzyme for acetyl phosphate-forming reaction1 Publication

    Pathwayi

    GO - Molecular functioni

    1. phosphate acetyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. acetyl-CoA biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-2353-MONOMER.
    BRENDAi2.3.1.8. 2169.
    UniPathwayiUPA00340; UER00459.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphate acetyltransferase (EC:2.3.1.8)
    Alternative name(s):
    Phosphotransacetylase
    Gene namesi
    Name:pta
    Ordered Locus Names:STM2338
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi252 – 2521R → H: Increases speed of forward and back reactions by 2-3 fold. Not inhibited by NADH. 1 Publication
    Mutagenesisi273 – 2731G → D: Increases speed of forward and back reactions by 2-3 fold. 1 Publication
    Mutagenesisi294 – 2941M → I: Slightly decreases speed of forward and back reactions. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 714713Phosphate acetyltransferasePRO_0000405551Add
    BLAST

    Proteomic databases

    PaxDbiQ8ZND6.
    PRIDEiQ8ZND6.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM2338.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8ZND6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni390 – 714325Phosphate acetyltransferaseAdd
    BLAST

    Domaini

    The N-terminal region seems to be important for proper quaternary structure. The C-terminal region contains the substrate-binding site By similarity.By similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the CobB/CobQ family.Curated
    In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0280.
    HOGENOMiHOG000053797.
    KOiK13788.
    OMAiKPIAQPH.
    OrthoDBiEOG6BKJ5W.
    PhylomeDBiQ8ZND6.

    Family and domain databases

    Gene3Di3.40.1390.20. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR010766. DRTGG.
    IPR016475. P-Actrans_bac.
    IPR027417. P-loop_NTPase.
    IPR004614. P_AcTrfase.
    IPR002505. PTA_PTB.
    IPR028979. Ser_kin/Pase_Hpr_N_like.
    [Graphical view]
    PfamiPF07085. DRTGG. 1 hit.
    PF01515. PTA_PTB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006107. PhpActrans_proteobac. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF75138. SSF75138. 1 hit.
    TIGRFAMsiTIGR00651. pta. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8ZND6-1 [UniParc]FASTAAdd to Basket

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    MSRIIMLIPT GTSVGLTSVS LGVIRAMERK GVRLSVFKPI AQPRAGGDAP    50
    DQTTTIVRAN STLPAAEPLK MSHVESLLSS NQKDVLMEEI IANYHANTKD 100
    AEVVLVEGLV PTRKHQFAQS LNYEIAKTLN AEIVFVMSQG TDTPEQLNER 150
    IELTRSSFGG AKNTNITGVI INKLNAPVDE QGRTRPDLSE IFDDSSKAQV 200
    IKIDPAKLQE SSPLPVLGAV PWSFDLIATR AIDMARHLNA TIINEGDIKT 250
    RRVKSVTFCA RSIPHMLEHF RAGSLLVTSA DRPDVLVAAC LAAMNGVEIG 300
    ALLLTGGYEM DARISKLCER AFATGLPVFM VNTNTWQTSL SLQSFNLEVP 350
    VDDHERIEKV QEYVANYVNA EWIESLTATS ERSRRLSPPA FRYQLTELAR 400
    KAGKRVVLPE GDEPRTVKAA AICAERGIAT CVLLGNPDEI NRVAASQGVE 450
    LGAGIEIVDP EVVRESYVAR LVELRKSKGM TEPVAREQLE DNVVLGTLML 500
    EQDEVDGLVS GAVHTTANTI RPPLQLIKTA PGSSLVSSVF FMLLPEQVYV 550
    YGDCAINPDP TAEQLAEIAI QSADSAIAFG IEPRVAMLSY STGTSGAGSD 600
    VEKVREATRL AQEKRPDLMI DGPLQYDAAV MADVAKSKAP NSPVAGRATV 650
    FIFPDLNTGN TTYKAVQRSA DLISIGPMLQ GMRKPVNDLS RGALVDDIVY 700
    TIALTAIQAS QQQQ 714
    Length:714
    Mass (Da):77,278
    Last modified:March 1, 2002 - v1
    Checksum:iD9DFE86F4AB21060
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL21239.1.
    RefSeqiNP_461280.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL21239; AAL21239; STM2338.
    GeneIDi1253860.
    KEGGistm:STM2338.
    PATRICi32383291. VBISalEnt20916_2475.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL21239.1 .
    RefSeqi NP_461280.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali Q8ZND6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM2338.

    Proteomic databases

    PaxDbi Q8ZND6.
    PRIDEi Q8ZND6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL21239 ; AAL21239 ; STM2338 .
    GeneIDi 1253860.
    KEGGi stm:STM2338.
    PATRICi 32383291. VBISalEnt20916_2475.

    Phylogenomic databases

    eggNOGi COG0280.
    HOGENOMi HOG000053797.
    KOi K13788.
    OMAi KPIAQPH.
    OrthoDBi EOG6BKJ5W.
    PhylomeDBi Q8ZND6.

    Enzyme and pathway databases

    UniPathwayi UPA00340 ; UER00459 .
    BioCyci SENT99287:GCTI-2353-MONOMER.
    BRENDAi 2.3.1.8. 2169.

    Family and domain databases

    Gene3Di 3.40.1390.20. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR010766. DRTGG.
    IPR016475. P-Actrans_bac.
    IPR027417. P-loop_NTPase.
    IPR004614. P_AcTrfase.
    IPR002505. PTA_PTB.
    IPR028979. Ser_kin/Pase_Hpr_N_like.
    [Graphical view ]
    Pfami PF07085. DRTGG. 1 hit.
    PF01515. PTA_PTB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006107. PhpActrans_proteobac. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF75138. SSF75138. 1 hit.
    TIGRFAMsi TIGR00651. pta. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    2. "Acetate excretion during growth of Salmonella enterica on ethanolamine requires phosphotransacetylase (EutD) activity, and acetate recapture requires acetyl-CoA synthetase (Acs) and phosphotransacetylase (Pta) activities."
      Starai V.J., Garrity J., Escalante-Semerena J.C.
      Microbiology 151:3793-3801(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: LT2.
    3. "In vivo and in vitro analyses of single-amino acid variants of the Salmonella enterica phosphotransacetylase enzyme provide insights into the function of its N-terminal domain."
      Brinsmade S.R., Escalante-Semerena J.C.
      J. Biol. Chem. 282:12629-12640(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ARG-252; GLY-273 AND MET-294.

    Entry informationi

    Entry nameiPTA_SALTY
    AccessioniPrimary (citable) accession number: Q8ZND6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3