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Protein

Phosphate acetyltransferase

Gene

pta

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in acetate metabolism. Catalyzes the reversible interconversion of acetyl-CoA and acetyl phosphate. The direction of the overall reaction changes depending on growth conditions. Required for acetate recapture but not for acetate excretion when this organism is grown on ethanolamine.1 Publication

Catalytic activityi

Acetyl-CoA + phosphate = CoA + acetyl phosphate.

Enzyme regulationi

Allosterically inhibited by NADH.1 Publication

Kineticsi

  1. KM=162.1 µM for acetyl-CoA1 Publication
  2. KM=329.3 µM for acetyl phosphate1 Publication
  1. Vmax=142.2 µM/h/mg enzyme for acetyl-CoA-forming reaction1 Publication
  2. Vmax=20.6 µM/h/mg enzyme for acetyl phosphate-forming reaction1 Publication

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes acetyl-CoA from acetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-2359-MONOMER
BRENDAi2.3.1.8 2169
UniPathwayiUPA00340; UER00459

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate acetyltransferase (EC:2.3.1.8)
Alternative name(s):
Phosphotransacetylase
Gene namesi
Name:pta
Ordered Locus Names:STM2338
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi252R → H: Increases speed of forward and back reactions by 2-3 fold. Not inhibited by NADH. 1 Publication1
Mutagenesisi273G → D: Increases speed of forward and back reactions by 2-3 fold. 1 Publication1
Mutagenesisi294M → I: Slightly decreases speed of forward and back reactions. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004055512 – 714Phosphate acetyltransferaseAdd BLAST713

Proteomic databases

PaxDbiQ8ZND6
PRIDEiQ8ZND6

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi99287.STM2338

Structurei

3D structure databases

ProteinModelPortaliQ8ZND6
SMRiQ8ZND6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni390 – 714Phosphate acetyltransferaseAdd BLAST325

Domaini

The N-terminal region seems to be important for proper quaternary structure. The C-terminal region contains the substrate-binding site (By similarity).By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the CobB/CobQ family.Curated
In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105C6K Bacteria
COG0280 LUCA
COG0857 LUCA
HOGENOMiHOG000053797
KOiK13788
OMAiTPLMFEY
PhylomeDBiQ8ZND6

Family and domain databases

Gene3Di3.40.1390.20, 1 hit
InterProiView protein in InterPro
IPR010766 DRTGG
IPR016475 P-Actrans_bac
IPR027417 P-loop_NTPase
IPR004614 P_AcTrfase
IPR002505 PTA_PTB
IPR028979 Ser_kin/Pase_Hpr-like_N_sf
PfamiView protein in Pfam
PF07085 DRTGG, 1 hit
PF01515 PTA_PTB, 1 hit
PIRSFiPIRSF006107 PhpActrans_proteobac, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF75138 SSF75138, 1 hit
TIGRFAMsiTIGR00651 pta, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8ZND6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRIIMLIPT GTSVGLTSVS LGVIRAMERK GVRLSVFKPI AQPRAGGDAP
60 70 80 90 100
DQTTTIVRAN STLPAAEPLK MSHVESLLSS NQKDVLMEEI IANYHANTKD
110 120 130 140 150
AEVVLVEGLV PTRKHQFAQS LNYEIAKTLN AEIVFVMSQG TDTPEQLNER
160 170 180 190 200
IELTRSSFGG AKNTNITGVI INKLNAPVDE QGRTRPDLSE IFDDSSKAQV
210 220 230 240 250
IKIDPAKLQE SSPLPVLGAV PWSFDLIATR AIDMARHLNA TIINEGDIKT
260 270 280 290 300
RRVKSVTFCA RSIPHMLEHF RAGSLLVTSA DRPDVLVAAC LAAMNGVEIG
310 320 330 340 350
ALLLTGGYEM DARISKLCER AFATGLPVFM VNTNTWQTSL SLQSFNLEVP
360 370 380 390 400
VDDHERIEKV QEYVANYVNA EWIESLTATS ERSRRLSPPA FRYQLTELAR
410 420 430 440 450
KAGKRVVLPE GDEPRTVKAA AICAERGIAT CVLLGNPDEI NRVAASQGVE
460 470 480 490 500
LGAGIEIVDP EVVRESYVAR LVELRKSKGM TEPVAREQLE DNVVLGTLML
510 520 530 540 550
EQDEVDGLVS GAVHTTANTI RPPLQLIKTA PGSSLVSSVF FMLLPEQVYV
560 570 580 590 600
YGDCAINPDP TAEQLAEIAI QSADSAIAFG IEPRVAMLSY STGTSGAGSD
610 620 630 640 650
VEKVREATRL AQEKRPDLMI DGPLQYDAAV MADVAKSKAP NSPVAGRATV
660 670 680 690 700
FIFPDLNTGN TTYKAVQRSA DLISIGPMLQ GMRKPVNDLS RGALVDDIVY
710
TIALTAIQAS QQQQ
Length:714
Mass (Da):77,278
Last modified:March 1, 2002 - v1
Checksum:iD9DFE86F4AB21060
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA Translation: AAL21239.1
RefSeqiNP_461280.1, NC_003197.2
WP_000086692.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL21239; AAL21239; STM2338
GeneIDi1253860
KEGGistm:STM2338
PATRICifig|99287.12.peg.2475

Similar proteinsi

Entry informationi

Entry nameiPTA_SALTY
AccessioniPrimary (citable) accession number: Q8ZND6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health