Q8ZMX9 (NADB_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-aspartate oxidase
Short name=LASPO
EC=1.4.3.16

Alternative name(s):
Quinolinate synthase B

Gene names

Name:	nadB
Ordered Locus Names:	STM2641

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC L-aspartate:fumarate oxidoreductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	L-aspartate oxidase		PRO_0000184400
Regions
	Nucleotide binding	12 – 26	15	FAD Potential
Sites
	Active site	244	1	By similarity
	Active site	263	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZMX9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: EE678152DB48B769	FASTA	540	60,109
	10 20 30 40 50 60 MMTTPELSCD VLIIGSGAAG LSLALRLAEK HKVIVLSKGP VSEGSTFYAQ GGIAAVFDET 70 80 90 100 110 120 DSIASHVEDT LIAGAGICDR HAVEFVASNA RTCVQWLIDQ GVLFDTHVQP NGKESYHLTR 130 140 150 160 170 180 EGGHSHRRIL HAADATGKEV ETTLVSRAQN HPNIQVLERS NAVDLIISDK MGLPGPRRVV 190 200 210 220 230 240 GAWIWNRNKE WVETCHAKSV VLATGGASKV YQYTTNPDIS SGDGIAMAWR AGCRVANLEF 250 260 270 280 290 300 NQFHPTALYH PQARNFLLTE ALRGEGAYLK RPDGSRFMPD VDERGELAPR DIVARAIDHE 310 320 330 340 350 360 MKQLGADCMF LDISHKPDDF VRQHFPMIYA KLLDLGMDLT KEPIPVVPAA HYTCGGVVVD 370 380 390 400 410 420 DYGRTDVDGL YAIGEVSYTG LHGANRMASN SLLECLVYGW SAAMDIDRRM PSVHSVDALP 430 440 450 460 470 480 AWDESRVENA DERVVIQHNW HELRLLMWDY VGIVRTTKRL ERALRRITML QQEIDEYYAN 490 500 510 520 530 540 FRVSNNLLEL RNLVQVAELI VRCAMMRKES RGLHFTLDYP QQLAESGPSI LSPLTPHINR

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL21535.1.
RefSeq	NP_461576.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q8ZMX9.
SMR	Q8ZMX9. Positions 5-533.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1254164.
GenomeReviews	Gene locus STM2641 in contig AE006468_GR.
KEGG	stm:STM2641.
NMPDR	fig|99287.1.peg.2555.
PATRIC	32383939. VBISalEnt20916_2791.
Phylogenomic databases
HOGENOM	HBG293998.
OMA	GAYIWNR.
ProtClustDB	PRK09077.
Enzyme and pathway databases
BioCyc	STYP99287:STM2641-MONOMER.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Gene3D	G3DSA:1.20.58.100. Fum_Rdtase/Succ_DH_flav-like_C. 1 hit.
KO	K00278.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF46977. Succ_DH_flav_C. 1 hit.
TIGRFAMs	TIGR00551. NadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_SALTY

Accession

Primary (citable) accession number: Q8ZMX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program