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Protein

Secreted effector protein PipB2

Gene

pipB2

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Involved in the reorganization of late endosome/lysosome (LE/Lys) compartments in mammalian cells. Necessary and sufficient to link kinesin-1 onto the Salmonella-containing vacuole (SCV) membrane. Required for centrifugal extension of lysosomal glycoprotein-rich membrane tubules, known as Salmonella-induced filaments (Sifs), away from the SCV and toward the cell periphery. Required for virulence, but not for intracellular survival and replication in phagocytic cells.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted effector protein PipB2
Alternative name(s):
Type III effector PipB2
Gene namesi
Name:pipB2
Ordered Locus Names:STM2780
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Secreted
  • Host membrane

  • Note: Secreted via the type III secretion system 2 (SPI-2 TTSS), and delivered into the host cell. In phagocytic cells localizes to the Salmonella-containing vacuole (SCV) and tubular extensions from the SCV, Salmonella-induced filaments (Sifs). In epithelial cells localizes to peripheral vesicles, in addition to SCVs and Sifs. Concentrates in detergent-resistant microdomains (DRMs), present on the intracellular membranes of SCVs and Sifs.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi340T → A: No effect. 1 Publication1
Mutagenesisi341L → A: Prevents the peripheral accumulation of GFP-PipB2 and LAMP-1. Slightly affects its LE/Lys redistribution. 1 Publication1
Mutagenesisi342F → A: Partial reduction in the peripheral accumulation of GFP-PipB2 and LAMP-1. 1 Publication1
Mutagenesisi343N → A: Increases LAMP-1 redistribution. No effect on peripheral accumulation of GFP-PipB2. 1 Publication1
Mutagenesisi344E → A: Decreases LAMP-1 redistribution. No effect on peripheral accumulation of GFP-PipB2. 1 Publication1
Mutagenesisi345F → A: Prevents LE/Lys redistribution. Does not affect the peripheral accumulation of GFP-PipB2. 1 Publication1
Mutagenesisi346Y → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782991 – 350Secreted effector protein PipB2Add BLAST350

Proteomic databases

PaxDbiQ8ZMM8.
PRIDEiQ8ZMM8.

Expressioni

Inductioni

Expression of the gene is regulated by the two-component regulatory system SsrA/SsrB. It is also activated by SlyA and controlled by the two-component regulatory system PhoP/PhoQ.

Interactioni

Subunit structurei

Interacts with the host kinesin light chain (KLC), a subunit of the kinesin-1 motor complex.1 Publication

Protein-protein interaction databases

DIPiDIP-61260N.
STRINGi99287.STM2780.

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 25Combined sources5
Helixi30 – 43Combined sources14
Beta strandi47 – 49Combined sources3
Helixi50 – 68Combined sources19
Beta strandi69 – 74Combined sources6
Beta strandi78 – 85Combined sources8
Beta strandi88 – 93Combined sources6
Beta strandi103 – 110Combined sources8
Beta strandi113 – 120Combined sources8
Helixi121 – 135Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LEZNMR-A17-161[»]
ProteinModelPortaliQ8ZMM8.
SMRiQ8ZMM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 201Pentapeptide repeat 1Add BLAST40
Domaini202 – 241Pentapeptide repeat 2Add BLAST40
Domaini247 – 286Pentapeptide repeat 3Add BLAST40
Domaini287 – 326Pentapeptide repeat 4Add BLAST40

Domaini

Contains various tandem pentapeptide repeats in the C-terminal region. The pentapeptide motif is required to efficiently recruit kinesin-1. No position is completely conserved in these repeats, whose consensus sequence is A-[DN]-[FLM]-X-X. The C-terminal 38 amino acids residues, specifically, the C-terminal motif LFNEF, are required for peripheral localization of PipB2 and redistribution of lysosomal-associated membrane protein (LAMP). The N-terminal 225 amino acids residues are sufficient for type III translocation and association with Sifs and SCVs, but not accumulation in peripheral vesicles.1 Publication

Sequence similaritiesi

Contains 4 pentapeptide repeat domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105VRE. Bacteria.
COG1357. LUCA.
HOGENOMiHOG000028269.
KOiK15352.
OMAiFNVFYSE.
PhylomeDBiQ8ZMM8.

Family and domain databases

InterProiIPR001646. 5peptide_repeat.
[Graphical view]
PfamiPF00805. Pentapeptide. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZMM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERSLDSLAG MAKSAFGAGT SAAMRQATSP KTILEYIINF FTCGGIRRRN
60 70 80 90 100
ETQYQELIET MAETLKSTMP DRGAPLPENI ILDDMDGCRV EFNLPGENNE
110 120 130 140 150
AGQVIVRVSK GDHSETREIP LASFEKICRA LLFRCEFSLP QDSVILTAQG
160 170 180 190 200
GMNLKGAVLT GANLTSENLC DADLSGANLE GAVLFMADCE GANFKGANLS
210 220 230 240 250
GTSLGDSNFK NACLEDSIMC GATLDHANLT GANLQHASLL GCSMIECNCS
260 270 280 290 300
GANMDHTNLS GATLIRADMS GATLQGATIM AAIMEGAVLT RANLRKASFI
310 320 330 340 350
STNLDGADLA EANLNNTCFK DCTLTDLRTE DATMSTSTQT LFNEFYSENI
Length:350
Mass (Da):37,274
Last modified:March 1, 2002 - v1
Checksum:i701D5EFBA7EB11EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY532917 Genomic DNA. Translation: AAS66036.1.
AE006468 Genomic DNA. Translation: AAL21665.1.
RefSeqiNP_461706.1. NC_003197.1.
WP_001738474.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21665; AAL21665; STM2780.
GeneIDi1254303.
KEGGistm:STM2780.
PATRICi32384228. VBISalEnt20916_2931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY532917 Genomic DNA. Translation: AAS66036.1.
AE006468 Genomic DNA. Translation: AAL21665.1.
RefSeqiNP_461706.1. NC_003197.1.
WP_001738474.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LEZNMR-A17-161[»]
ProteinModelPortaliQ8ZMM8.
SMRiQ8ZMM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61260N.
STRINGi99287.STM2780.

Proteomic databases

PaxDbiQ8ZMM8.
PRIDEiQ8ZMM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL21665; AAL21665; STM2780.
GeneIDi1254303.
KEGGistm:STM2780.
PATRICi32384228. VBISalEnt20916_2931.

Phylogenomic databases

eggNOGiENOG4105VRE. Bacteria.
COG1357. LUCA.
HOGENOMiHOG000028269.
KOiK15352.
OMAiFNVFYSE.
PhylomeDBiQ8ZMM8.

Family and domain databases

InterProiIPR001646. 5peptide_repeat.
[Graphical view]
PfamiPF00805. Pentapeptide. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIPB2_SALTY
AccessioniPrimary (citable) accession number: Q8ZMM8
Secondary accession number(s): Q6QNB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.