Q8ZMH3 (PRP2_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2
EC=3.1.3.16

Gene names

Name:	pphB
Synonyms:	prpB
Ordered Locus Names:	STM2907

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can hydrolyze phosphorylated Ser-, Thr- or Tyr-substrates in vitro. The natural substrate is unknown.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Manganese.
Enzyme regulation	Inhibited by cadmium, copper, zinc when added concomitantly with manganese. Nickel, cobalt and iron support activity but with less efficiency.
Miscellaneous	Neither magnesium nor calcium stimulates activity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5-8.5. Temperature dependence: Optimum temperature is 30-37 degrees Celsius.

Ontologies

Keywords
Ligand	Manganese
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Serine/threonine-protein phosphatase 2		PRO_0000058911
Sites
	Active site	78	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZMH3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 723B98837426E350	FASTA	218	25,025
	10 20 30 40 50 60 MELIRYADIN SDLYRHIWVV GDIHGCYSLL LTRLAQLNFS PDTDLLISTG DNIDRGKENL 70 80 90 100 110 120 ETLRLLNTPW FISVVGNHEA MALDAFETQD GNFWYVNGGY WYDSVTEKDR QEATELLLTF 130 140 150 160 170 180 KQRPHIIEVE TSSKKYVIAH ADYPDDSYDY GKQVDIDSVL WSRDRLLGSL QGNIHPIRGA 190 200 210 DTFIFGHMIV DYTTTFANQI YIDTGSFCSG NLSFFKIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties." Shi L., Kehres D.G., Maguire M.E. J. Bacteriol. 183:7053-7057(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY049951 Genomic DNA. Translation: AAL09832.1. AE006468 Genomic DNA. Translation: AAL21787.1.
RefSeq	NP_461828.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q8ZMH3.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM2907.
Protein family/group databases
PptaseDB	P3D0411149.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL21787; AAL21787; STM2907.
GeneID	1254430.
KEGG	stm:STM2907.
PATRIC	32384498. VBISalEnt20916_3061.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000251869.
KO	K07314.
OMA	NTSWFIS.
ProtClustDB	PRK09968.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRP2_SALTY

Accession

Primary (citable) accession number: Q8ZMH3
Secondary accession number(s): Q79S89

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program