Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8ZMH3 (PRP2_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2

EC=3.1.3.16
Gene names
Name:pphB
Synonyms:prpB
Ordered Locus Names:STM2907
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can hydrolyze phosphorylated Ser-, Thr- or Tyr-substrates in vitro. The natural substrate is unknown.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Manganese.

Enzyme regulation

Inhibited by cadmium, copper, zinc when added activity but with less efficiency.

Miscellaneous

Neither magnesium nor calcium stimulates activity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.5-8.5.

Temperature dependence:

Optimum temperature is 30-37 degrees Celsius.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 218218Serine/threonine-protein phosphatase 2
PRO_0000058911

Sites

Active site781Proton donor By similarity
Metal binding221Manganese 1 By similarity
Metal binding241Manganese 1 By similarity
Metal binding511Manganese 1 By similarity
Metal binding511Manganese 2 By similarity
Metal binding771Manganese 2 By similarity
Metal binding1871Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ZMH3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 723B98837426E350

FASTA21825,025
        10         20         30         40         50         60 
MELIRYADIN SDLYRHIWVV GDIHGCYSLL LTRLAQLNFS PDTDLLISTG DNIDRGKENL 

        70         80         90        100        110        120 
ETLRLLNTPW FISVVGNHEA MALDAFETQD GNFWYVNGGY WYDSVTEKDR QEATELLLTF 

       130        140        150        160        170        180 
KQRPHIIEVE TSSKKYVIAH ADYPDDSYDY GKQVDIDSVL WSRDRLLGSL QGNIHPIRGA 

       190        200        210 
DTFIFGHMIV DYTTTFANQI YIDTGSFCSG NLSFFKIK 

« Hide

References

« Hide 'large scale' references
[1]"The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties."
Shi L., Kehres D.G., Maguire M.E.
J. Bacteriol. 183:7053-7057(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY049951 Genomic DNA. Translation: AAL09832.1.
AE006468 Genomic DNA. Translation: AAL21787.1.
RefSeqNP_461828.1. NC_003197.1.

3D structure databases

ProteinModelPortalQ8ZMH3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM2907.

Protein family/group databases

PptaseDBP3D0411149.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL21787; AAL21787; STM2907.
GeneID1254430.
KEGGstm:STM2907.
PATRIC32384498. VBISalEnt20916_3061.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000251869.
KOK07314.
OMAGDWFFEL.
OrthoDBEOG6KDKSC.
PhylomeDBQ8ZMH3.

Enzyme and pathway databases

BioCycSENT99287:GCTI-2923-MONOMER.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
ProtoNetSearch...

Entry information

Entry namePRP2_SALTY
AccessionPrimary (citable) accession number: Q8ZMH3
Secondary accession number(s): Q79S89
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families