Reviewed, UniProtKB/Swiss-Prot Q8ZMF9 (PIMT_SALTY)

Names and origin

Protein names

Recommended name:
    Protein-L-isoaspartate O-methyltransferase
    EC=2.1.1.77
Alternative name(s):
    Protein-beta-aspartate methyltransferase
      Short name=PIMT
    Protein L-isoaspartyl methyltransferase
    L-isoaspartyl protein carboxyl methyltransferase

Gene names

Name:	pcm
Ordered Locus Names:	STM2926

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: HAMAP protein repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Protein-L-isoaspartate O-methyltransferase HAMAP MF_00090		PRO_0000111903
Sites
	Active site	59	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZMF9-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4CC10D557A37FAB7	FASTA	208	23,192
	10 20 30 40 50 60 MVSGRVQALL EQLRAQGIRD EQVLNALAAV PREKFIDEAF EHKAWENIAL PIGQGQTISQ 70 80 90 100 110 120 PYMVARMTEL LELTPQSRVL EIGTGSGYQT AILAHLVHHV CSVERIKGLQ WQARRRLKQL 130 140 150 160 170 180 DLHNVSTRHG DGWQGWQARA PFDAIIVTAA PPEIPTALMA QLDEGGILVL PVGDEQQFLK 190 200 RVRRRGGEFI IDTVEAVRFV PLVKGELA

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008833 Genomic DNA. Translation: AAL21806.1.
RefSeq	NP_461847.1.
3D structure databases
HSSP	HSSP built from PDB template 1JG1 based on UniProtKB Q8TZR3.
ModBase	Search...
Genome annotation databases
GeneID	1254449.
GenomeReviews	Gene locus STM2926 in contig AE006468_GR.
KEGG	stm:STM2926.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZMF9.
OMA	Q8ZMF9. YMVARMS.
Enzyme and pathway databases
BioCyc	STYP99287:STM2926-MON.
BRENDA	2.1.1.77. 2.
Family and domain databases
HAMAP	MF_00090. [Tree]
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_SALTY

Accession

Primary (citable) accession number: Q8ZMF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)