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Q8ZM86 (VAPC_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA(fMet)-specific endonuclease VapC

EC=3.1.-.-
Alternative name(s):
RNase VapC
Toxin VapC
Gene names
Name:vapC
Ordered Locus Names:STM3033
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length132 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Toxic component of a toxin-antitoxin (TA) module. A site-specific tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Does not cleave tRNA(Met), tRNA(Arg2), tRNA(His), tRNA(Leu), tRNA(Phe) tRNA(Thr1), tRNA(Tyr) or tRNA(Val). Overexpression in E.coli inhibits translation, leads to loss of cell growth and degradation of tRNA(fMet), these effects are neutralized by expression of cognate antitoxin VapB. Expression also activates translation initiation at correctly positioned elongator codons (AAA and to a lesser extent AAG). Ref.2 Ref.3

Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity. Ref.2 Ref.3

Cofactor

Magnesium Potential.

Subunit structure

Forms a complex with antitoxin VapB Probable.

Induction

Induced by amino acid starvation and by chloramphenicol treatment. Ref.2

Sequence similarities

Belongs to the PINc/VapC protein family.

Contains 1 PINc domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 132132tRNA(fMet)-specific endonuclease VapC HAMAP-Rule MF_00265
PRO_0000410980

Regions

Domain5 – 131127PINc

Sites

Metal binding71Magnesium Potential
Metal binding981Magnesium Potential

Experimental info

Mutagenesis71D → A: No inhibition of cell growth, no degradation of tRNA(fMet). Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8ZM86 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 4AA4120DAD6B6057

FASTA13214,938
        10         20         30         40         50         60 
MLKFMLDTNT CIFTIKNKPE HIRERFNLNT SRMCISSITL MELIYGAEKS LAPERNLAVV 

        70         80         90        100        110        120 
EGFISRLEVL DYDTQAAIHT GQIRAELARK GTPVGPYDQM IAGHAGSRGL VVVTNNLREF 

       130 
ERIPGIRIED WC 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[2]"Ectopic production of VapCs from Enterobacteria inhibits translation and trans-activates YoeB mRNA interferase."
Winther K.S., Gerdes K.
Mol. Microbiol. 72:918-930(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TOXIN, INDUCTION, EXPRESSION IN E.COLI.
Strain: LT2 / SGSC1412 / ATCC 700720.
[3]"Enteric virulence associated protein VapC inhibits translation by cleavage of initiator tRNA."
Winther K.S., Gerdes K.
Proc. Natl. Acad. Sci. U.S.A. 108:7403-7407(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TOXIN, FUNCTION AS A TRNA ENDONUCLEASE, SUBSTRATE SPECIFICITY, MUTAGENESIS OF ASP-7.
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006468 Genomic DNA. Translation: AAL21909.1.
RefSeqNP_461950.1. NC_003197.1.

3D structure databases

ProteinModelPortalQ8ZM86.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM3033.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL21909; AAL21909; STM3033.
GeneID1254556.
KEGGstm:STM3033.
PATRIC32384803. VBISalEnt20916_3211.

Phylogenomic databases

HOGENOMHOG000121274.
KOK07062.
OMADFGIPAI.
OrthoDBEOG6ZWJH4.
ProtClustDBPRK13725.

Enzyme and pathway databases

BioCycSENT99287:GCTI-3053-MONOMER.

Family and domain databases

HAMAPMF_00265. VapC_Nob1.
InterProIPR002716. PIN_dom.
IPR022907. VapC_family.
[Graphical view]
PfamPF01850. PIN. 1 hit.
[Graphical view]
SMARTSM00670. PINc. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVAPC_SALTY
AccessionPrimary (citable) accession number: Q8ZM86
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families