Reviewed, UniProtKB/Swiss-Prot Q8ZM06 (DKGA_SALTY)

Names and origin

Protein names

Recommended name:
    2,5-diketo-D-gluconic acid reductase A
      Short name=2,5-DKG reductase A
      Short name=2,5-DKGR A
      Short name=25DKGR-A
    EC=1.1.1.274
Alternative name(s):
    AKR5C

Gene names

Name:	dkgA
Ordered Locus Names:	STM3165

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) By similarity.
Catalytic activity	2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Ascorbate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-ascorbic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,5-didehydrogluconate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	2,5-diketo-D-gluconic acid reductase A		PRO_0000124602
Regions
	Nucleotide binding	187 – 241	55	NADP By similarity
Sites
	Active site	51	1	Proton donor By similarity
	Binding site	107	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZM06-1 [UniParc]. Last modified March 27, 2002. Version 1. Checksum: 84E0E6C8901544AE	FASTA	275	30,996
	10 20 30 40 50 60 MANPTIIRLQ DGNVMPQLGL GVWKASNEEV IAAIHKALEV GYRSIDTATA YQNEEGVGKA 70 80 90 100 110 120 LKAASVAREE LFITTKLWND DQKRPREALQ ESLKKLQLDY LDLYLMHWPV PAIDHYVDAW 130 140 150 160 170 180 KGMIALQKEG LVKSIGVCNF QIHHLQRLID ETGVTPVINQ IELHPLMQQR QLHAWNATHK 190 200 210 220 230 240 IQTESWSPLA QGGEGVFDQK VIRELADKYG KTPAQIVIRW HLDCGLVVIP KSVTPSRIAE 250 260 270 NFAVWDFRLD KDELGEIAKL DQGKRLGPDP DQFGG

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008845 Genomic DNA. Translation: AAL22039.1.
RefSeq	NP_462080.1.
3D structure databases
HSSP	HSSP built from PDB template 1HW6 based on UniProtKB P06632.
SMR	Q8ZM06. Positions 1-275.
ModBase	Search...
Genome annotation databases
GeneID	1254688.
GenomeReviews	Gene locus STM3165 in contig AE006468_GR.
KEGG	stm:STM3165.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZM06.
OMA	Q8ZM06. HLERIIT.
Enzyme and pathway databases
BioCyc	STYP99287:STM3165-MON.
BRENDA	1.1.1.274. 2.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DKGA_SALTY

Accession

Primary (citable) accession number: Q8ZM06

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)