Reviewed, UniProtKB/Swiss-Prot Q8ZLI0 (RTCA_SALTY)

Names and origin

Protein names

Recommended name:
    RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	STM3518

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156421
Sites
	Active site	308	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZLI0-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 182667CD81E31125	FASTA	339	35,458
	10 20 30 40 50 60 MARIIALDGA QGEGGGQILR SALSLSMITG QPFEMSGIRA GRAKPGLLRQ HLTAVRAATE 70 80 90 100 110 120 ICGAQVNGDE LGSQQLRFTP GPIRGGEYRF AIGSAGSCML VLQTVLPALW FADGSSRVEV 130 140 150 160 170 180 HGGTHNQAAP SADFICRVWE PLLARMGISQ RTTLIKHGFY PAGGGAAATV VEPAASLRGL 190 200 210 220 230 240 TLISRGETLR TTAEALLAAV PYHVGEREVA TLEAHFPQAE KNVVALEGGC GPGNALSLMI 250 260 270 280 290 300 QSEQLTELFA AFGVKGTSAE AVANQVAHEA RRYLASPAAV GEHLADQLIL PLALAGEGAF 310 320 330 TVARASAHLL TNIAVVERFL PVRFSCEATE SGYLVRVSD

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006468 Genomic DNA. Translation: AAL22380.1.
RefSeq	NP_462421.1.
3D structure databases
HSSP	HSSP built from PDB template 1QMH based on UniProtKB P46849.
ModBase	Search...
Genome annotation databases
GeneID	1255041.
GenomeReviews	Gene locus STM3518 in contig AE006468_GR.
KEGG	stm:STM3518.
NMPDR	fig|99287.1.peg.3398.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZLI0.
OMA	EIDGSYG.
Enzyme and pathway databases
BioCyc	STYP99287:STM3518-MON.
BRENDA	6.5.1.4. 2.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_SALTY

Accession

Primary (citable) accession number: Q8ZLI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)