ID   GUN_SALTY               Reviewed;         369 AA.
AC   Q8ZLB7; Q8VQ10; Q93IN0;
DT   26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   27-MAR-2024, entry version 117.
DE   RecName: Full=Endoglucanase;
DE            EC=3.2.1.4;
DE   AltName: Full=Carboxymethylcellulase;
DE            Short=CMCase;
DE   AltName: Full=Cellulase;
DE   AltName: Full=Endo-1,4-beta-glucanase;
DE   Flags: Precursor;
GN   Name=bcsZ; Synonyms=bcsC, celC; OrderedLocusNames=STM3617;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 14028 / SGSG 2980 / CDC 6516-60 / NCTC 12023;
RX   PubMed=11260463; DOI=10.1046/j.1365-2958.2001.02337.x;
RA   Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U.;
RT   "The multicellular morphotypes of Salmonella typhimurium and Escherichia
RT   coli produce cellulose as the second component of the extracellular
RT   matrix.";
RL   Mol. Microbiol. 39:1452-1463(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UR1;
RA   Yoo J.-S., Chung S.-Y., Choi Y.-L.;
RT   "Cloning and sequencing of CMCase gene from Salmonella typhimurium UR1.";
RL   Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=11929533; DOI=10.1046/j.1365-2958.2002.02802.x;
RA   Solano C., Garcia B., Valle J., Berasain C., Ghigo J.-M., Gamazo C.,
RA   Lasa I.;
RT   "Genetic analysis of Salmonella enteritidis biofilm formation: critical
RT   role of cellulose.";
RL   Mol. Microbiol. 43:793-808(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Hydrolyzes carboxymethylcellulose. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 8 (cellulase D) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL69395.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAC44017.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AJ315770; CAC44017.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AF464897; AAL69395.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AJ315148; CAC86197.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL22477.1; -; Genomic_DNA.
DR   RefSeq; NP_462518.1; NC_003197.2.
DR   RefSeq; WP_000988813.1; NC_003197.2.
DR   AlphaFoldDB; Q8ZLB7; -.
DR   SMR; Q8ZLB7; -.
DR   STRING; 99287.STM3617; -.
DR   CAZy; GH8; Glycoside Hydrolase Family 8.
DR   PaxDb; 99287-STM3617; -.
DR   GeneID; 1255140; -.
DR   KEGG; stm:STM3617; -.
DR   PATRIC; fig|99287.12.peg.3823; -.
DR   HOGENOM; CLU_037297_0_0_6; -.
DR   OMA; IRVYLWV; -.
DR   PhylomeDB; Q8ZLB7; -.
DR   BioCyc; SENT99287:STM3617-MONOMER; -.
DR   UniPathway; UPA00694; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR002037; Glyco_hydro_8.
DR   InterPro; IPR019834; Glyco_hydro_8_CS.
DR   Pfam; PF01270; Glyco_hydro_8; 1.
DR   PRINTS; PR00735; GLHYDRLASE8.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00812; GLYCOSYL_HYDROL_F8; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..369
FT                   /note="Endoglucanase"
FT                   /id="PRO_0000007941"
FT   ACT_SITE        56
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        117
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10058"
FT   CONFLICT        184
FT                   /note="L -> Q (in Ref. 2; AAL69395)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        366..369
FT                   /note="VSSR -> ANSH (in Ref. 2; AAL69395)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   369 AA;  41755 MW;  6732DE30EA6332C6 CRC64;
     MMTMLRGWIT MLVMLTAINA QAACSWPAWE QFKKDYISQQ GRVIDPGDAR KITTSEGQSY
     AMFFALAAND RPAFAQLFNW TQNNLAQGSL REYLPAWLWG QKDPDTWSVL DSNSASDGDI
     WMAWSLLEAG RLWKETRYTE VGTALLKRIA REEVLNVPGL GSMLLPGKIG FAEANSWRFN
     PSYLPPQLAQ YFSRFGAPWS TLRETNLRLL LETAPKGFSP DWVRYESKQG WQLKAEKTLI
     SSYDAIRVYL WAGMMHDGDP QKARLLARFK PMATLTMKNG VPPEKVDVVS GNAQGTGPVG
     FSAALLPFLQ NRDAQAVQRQ RVADHFPGSD AYYNYVLTLF GQGWDQHRFR FTVKGELLPD
     WGQECVSSR
//