Q8ZLB7 (GUN_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase
Short name=CMCase
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:	bcsZ
Synonyms:	bcsC, celC
Ordered Locus Names:	STM3617

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes carboxymethylcellulose By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 8 (cellulase D) family.
Sequence caution	The sequence AAL69395.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC44017.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 369	347	Endoglucanase		PRO_0000007941
Sites
	Active site	56	1	Proton donor By similarity
	Active site	117	1	Nucleophile Potential
Experimental info
	Sequence conflict	184	1	L → Q in AAL69395. Ref.2
	Sequence conflict	366 – 369	4	VSSR → ANSH in AAL69395. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8ZLB7 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 6732DE30EA6332C6	FASTA	369	41,755
	10 20 30 40 50 60 MMTMLRGWIT MLVMLTAINA QAACSWPAWE QFKKDYISQQ GRVIDPGDAR KITTSEGQSY 70 80 90 100 110 120 AMFFALAAND RPAFAQLFNW TQNNLAQGSL REYLPAWLWG QKDPDTWSVL DSNSASDGDI 130 140 150 160 170 180 WMAWSLLEAG RLWKETRYTE VGTALLKRIA REEVLNVPGL GSMLLPGKIG FAEANSWRFN 190 200 210 220 230 240 PSYLPPQLAQ YFSRFGAPWS TLRETNLRLL LETAPKGFSP DWVRYESKQG WQLKAEKTLI 250 260 270 280 290 300 SSYDAIRVYL WAGMMHDGDP QKARLLARFK PMATLTMKNG VPPEKVDVVS GNAQGTGPVG 310 320 330 340 350 360 FSAALLPFLQ NRDAQAVQRQ RVADHFPGSD AYYNYVLTLF GQGWDQHRFR FTVKGELLPD WGQECVSSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix." Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U. Mol. Microbiol. 39:1452-1463(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14028 / SGSG 2980 / CDC 6516-60 / NCTC 12023.
[2]	"Cloning and sequencing of CMCase gene from Salmonella typhimurium UR1." Yoo J.-S., Chung S.-Y., Choi Y.-L. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: UR1.
[3]	"Genetic analysis of Salmonella enteritidis biofilm formation: critical role of cellulose." Solano C., Garcia B., Valle J., Berasain C., Ghigo J.-M., Gamazo C., Lasa I. Mol. Microbiol. 43:793-808(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[4]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ315770 Genomic DNA. Translation: CAC44017.1. Different initiation. AF464897 Genomic DNA. Translation: AAL69395.1. Different initiation. AJ315148 Genomic DNA. Translation: CAC86197.1. AE006468 Genomic DNA. Translation: AAL22477.1.
RefSeq	NP_462518.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q8ZLB7.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM3617.
Protein family/group databases
CAZy	GH8. Glycoside Hydrolase Family 8.
Proteomic databases
PaxDb	Q8ZLB7.
PRIDE	Q8ZLB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL22477; AAL22477; STM3617.
GeneID	1255140.
KEGG	stm:STM3617.
PATRIC	32386051. VBISalEnt20916_3823.
Phylogenomic databases
eggNOG	COG3405.
HOGENOM	HOG000259088.
KO	K01179.
OMA	IRVYLWV.
ProtClustDB	PRK11097.
Enzyme and pathway databases
UniPathway	UPA00694.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR002037. Glyco_hydro_8. IPR019834. Glyco_hydro_8_CS. [Graphical view]
Pfam	PF01270. Glyco_hydro_8. 1 hit. [Graphical view]
PRINTS	PR00735. GLHYDRLASE8.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00812. GLYCOSYL_HYDROL_F8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_SALTY

Accession

Primary (citable) accession number: Q8ZLB7
Secondary accession number(s): Q8VQ10, Q93IN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program