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Protein

Endoglucanase

Gene

bcsZ

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes carboxymethylcellulose.By similarity

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561Proton donorBy similarity
Active sitei117 – 1171NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciSENT99287:GCTI-3640-MONOMER.
UniPathwayiUPA00694.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Carboxymethylcellulase
Short name:
CMCase
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:bcsZ
Synonyms:bcsC, celC
Ordered Locus Names:STM3617
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 369347EndoglucanasePRO_0000007941Add
BLAST

Proteomic databases

PaxDbiQ8ZLB7.
PRIDEiQ8ZLB7.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM3617.

Structurei

3D structure databases

ProteinModelPortaliQ8ZLB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3405.
HOGENOMiHOG000259088.
KOiK01179.
OMAiIRVYLWV.
OrthoDBiEOG6DG2PM.
PhylomeDBiQ8ZLB7.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8ZLB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTMLRGWIT MLVMLTAINA QAACSWPAWE QFKKDYISQQ GRVIDPGDAR
60 70 80 90 100
KITTSEGQSY AMFFALAAND RPAFAQLFNW TQNNLAQGSL REYLPAWLWG
110 120 130 140 150
QKDPDTWSVL DSNSASDGDI WMAWSLLEAG RLWKETRYTE VGTALLKRIA
160 170 180 190 200
REEVLNVPGL GSMLLPGKIG FAEANSWRFN PSYLPPQLAQ YFSRFGAPWS
210 220 230 240 250
TLRETNLRLL LETAPKGFSP DWVRYESKQG WQLKAEKTLI SSYDAIRVYL
260 270 280 290 300
WAGMMHDGDP QKARLLARFK PMATLTMKNG VPPEKVDVVS GNAQGTGPVG
310 320 330 340 350
FSAALLPFLQ NRDAQAVQRQ RVADHFPGSD AYYNYVLTLF GQGWDQHRFR
360
FTVKGELLPD WGQECVSSR
Length:369
Mass (Da):41,755
Last modified:March 1, 2002 - v1
Checksum:i6732DE30EA6332C6
GO

Sequence cautioni

The sequence AAL69395.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAC44017.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841L → Q in AAL69395 (Ref. 2) Curated
Sequence conflicti366 – 3694VSSR → ANSH in AAL69395 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ315770 Genomic DNA. Translation: CAC44017.1. Different initiation.
AF464897 Genomic DNA. Translation: AAL69395.1. Different initiation.
AJ315148 Genomic DNA. Translation: CAC86197.1.
AE006468 Genomic DNA. Translation: AAL22477.1.
RefSeqiNP_462518.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL22477; AAL22477; STM3617.
GeneIDi1255140.
KEGGistm:STM3617.
PATRICi32386051. VBISalEnt20916_3823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ315770 Genomic DNA. Translation: CAC44017.1. Different initiation.
AF464897 Genomic DNA. Translation: AAL69395.1. Different initiation.
AJ315148 Genomic DNA. Translation: CAC86197.1.
AE006468 Genomic DNA. Translation: AAL22477.1.
RefSeqiNP_462518.1. NC_003197.1.

3D structure databases

ProteinModelPortaliQ8ZLB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM3617.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Proteomic databases

PaxDbiQ8ZLB7.
PRIDEiQ8ZLB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL22477; AAL22477; STM3617.
GeneIDi1255140.
KEGGistm:STM3617.
PATRICi32386051. VBISalEnt20916_3823.

Phylogenomic databases

eggNOGiCOG3405.
HOGENOMiHOG000259088.
KOiK01179.
OMAiIRVYLWV.
OrthoDBiEOG6DG2PM.
PhylomeDBiQ8ZLB7.

Enzyme and pathway databases

UniPathwayiUPA00694.
BioCyciSENT99287:GCTI-3640-MONOMER.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix."
    Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U.
    Mol. Microbiol. 39:1452-1463(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 14028 / SGSG 2980 / CDC 6516-60 / NCTC 12023.
  2. "Cloning and sequencing of CMCase gene from Salmonella typhimurium UR1."
    Yoo J.-S., Chung S.-Y., Choi Y.-L.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: UR1.
  3. "Genetic analysis of Salmonella enteritidis biofilm formation: critical role of cellulose."
    Solano C., Garcia B., Valle J., Berasain C., Ghigo J.-M., Gamazo C., Lasa I.
    Mol. Microbiol. 43:793-808(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiGUN_SALTY
AccessioniPrimary (citable) accession number: Q8ZLB7
Secondary accession number(s): Q8VQ10, Q93IN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.