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Q8ZL83 (DLGD_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2,3-diketo-L-gulonate reductase
Short name=2,3-DKG reductase
EC=1.1.1.130
Alternative name(s):
3-dehydro-L-gulonate 2-dehydrogenase
Gene names
Name:dlgD
Ordered Locus Names:STM3668
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate By similarity. HAMAP-Rule MF_00820

Catalytic activity

3-dehydro-L-gulonate + NAD(P)+ = (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H. HAMAP-Rule MF_00820

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00820.

Sequence similarities

Belongs to the LDH2/MDH2 oxidoreductase family. DlgD subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function3-dehydro-L-gulonate 2-dehydrogenase activity

Inferred from electronic annotation. Source: HAMAP

NAD+ binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3323322,3-diketo-L-gulonate reductase HAMAP-Rule MF_00820
PRO_0000083838

Regions

Nucleotide binding168 – 1747NAD By similarity
Nucleotide binding224 – 2252NAD By similarity
Nucleotide binding304 – 3063NAD By similarity

Sites

Active site441Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ZL83 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 44989A7C8613E7FD

FASTA33236,771
        10         20         30         40         50         60 
MKVTFEELKG AFYRVLRSRN IAEDTADECA EMFARTTESG VYSHGVNRFP RFIQQLDNGD 

        70         80         90        100        110        120 
IIPDAKPQRV TSLGAIEQWD AQRAIGNLTA KKMMDRAIEL ASDHGIGLVA LRNANHWMRG 

       130        140        150        160        170        180 
GSYGWQAAEK GYIGICWTNS IAVMPPWGAK ECRIGTNPLI VAIPSTPITM VDMSMSMFSY 

       190        200        210        220        230        240 
GMLEVNRLAG RELPVDGGFD DNGQLTKEPG VIEKNRRILP MGYWKGSGLS IVLDMIATLL 

       250        260        270        280        290        300 
SNGSSVAEVT QENSDEYGVS QIFIAIEVDK LIDGATRDAK LQRIMDFITT AERADDNVAI 

       310        320        330 
RLPGHEFTKL LDDNRRHGIT IDDSVWAKIQ AL

« Hide

References

[1]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE006468 Genomic DNA. Translation: AAL22527.1.
RefSeqNP_462568.1. NC_003197.1.

3D structure databases

ProteinModelPortalQ8ZL83.
SMRQ8ZL83. Positions 1-332.
ModBaseSearch...

Protein-protein interaction databases

STRING99287.STM3668.

Proteomic databases

PaxDbQ8ZL83.
PRIDEQ8ZL83.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL22527; AAL22527; STM3668.
GeneID1255192.
KEGGstm:STM3668.
PATRIC32386169. VBISalEnt20916_3881.

Phylogenomic databases

eggNOGCOG2055.
HOGENOMHOG000249660.
KOK08092.
OMAVDERVWA.
ProtClustDBPRK13260.

Family and domain databases

HAMAPMF_00820. Diketo_gul_reduc.
InterProIPR023689. Diketo_gul_Rdtase.
IPR003767. Malate/L-lactate_DH.
[Graphical view]
PANTHERPTHR11091. PTHR11091. 1 hit.
PfamPF02615. Ldh_2. 1 hit.
[Graphical view]
SUPFAMSSF89733. MDH_LDH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDLGD_SALTY
AccessionPrimary (citable) accession number: Q8ZL83
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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