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Protein

tRNA (cytidine(34)-2'-O)-methyltransferase

Gene

yibK

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu).UniRule annotation
S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei100S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei122S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei130S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processtRNA processingUniRule annotation
LigandS-adenosyl-L-methionineUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation)
Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmLUniRule annotation
Gene namesi
Name:yibKImported
Synonyms:trmLUniRule annotation
Ordered Locus Names:STM3695Imported
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)Imported
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PaxDbiQ8ZL60.
PRIDEiQ8ZL60.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM3695.

Structurei

3D structure databases

ProteinModelPortaliQ8ZL60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 142SpoU_methylaseInterPro annotationAdd BLAST141

Sequence similaritiesi

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UIQ. Bacteria.
COG0219. LUCA.
HOGENOMiHOG000272756.
KOiK03216.
OMAiAGLDYWH.
PhylomeDBiQ8ZL60.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_01885. tRNA_methyltr_TrmL. 1 hit.
InterProiView protein in InterPro
IPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR016914. tRNA_cyt/urid_MeTfrase.
IPR029026. tRNA_m1G_MTases_N.
PANTHERiPTHR42971. PTHR42971. 1 hit.
PfamiView protein in Pfam
PF00588. SpoU_methylase. 1 hit.
PIRSFiPIRSF029256. SpoU_TrmH_prd. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00185. tRNA_yibK_trmL. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZL60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNIVLFEPE IPPNTGNIIR LCANTGFRLH IIEPMGFTWD DKRLRRAGLD
60 70 80 90 100
YHEFAAVQRH HDYAAFVAAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG
110 120 130 140 150
PETRGLPASI LDALPKEQKI RIPMMPDSRS MNLSNAVSVV VYEAWRQLGY

PGAVLRS
Length:157
Mass (Da):17,641
Last modified:March 1, 2002 - v1
Checksum:iAD0E1D36BE19541F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL22554.1.
RefSeqiNP_462595.1. NC_003197.2.
WP_000932335.1. NC_003197.2.

Genome annotation databases

EnsemblBacteriaiAAL22554; AAL22554; STM3695.
GeneIDi1255219.
KEGGistm:STM3695.
PATRICifig|99287.12.peg.3908.

Similar proteinsi

Entry informationi

Entry nameiQ8ZL60_SALTY
AccessioniPrimary (citable) accession number: Q8ZL60
Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2002
Last sequence update: March 1, 2002
Last modified: September 27, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported