Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (cytidine(34)-2'-O)-methyltransferase

Gene

yibK

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu).UniRule annotation
S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei100S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei122S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei130S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processtRNA processingUniRule annotation
LigandS-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-3732-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation)
Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmLUniRule annotation
Gene namesi
Name:yibKImported
Synonyms:trmLUniRule annotation
Ordered Locus Names:STM3695Imported
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)Imported
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PaxDbiQ8ZL60
PRIDEiQ8ZL60

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM3695

Structurei

3D structure databases

ProteinModelPortaliQ8ZL60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 142SpoU_methylaseInterPro annotationAdd BLAST141

Sequence similaritiesi

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UIQ Bacteria
COG0219 LUCA
HOGENOMiHOG000272756
KOiK03216
OMAiAGLDYWH
PhylomeDBiQ8ZL60

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
HAMAPiMF_01885 tRNA_methyltr_TrmL, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR001537 SpoU_MeTrfase
IPR016914 tRNA_cyt/urid_MeTfrase
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR42971 PTHR42971, 1 hit
PfamiView protein in Pfam
PF00588 SpoU_methylase, 1 hit
PIRSFiPIRSF029256 SpoU_TrmH_prd, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00185 tRNA_yibK_trmL, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZL60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNIVLFEPE IPPNTGNIIR LCANTGFRLH IIEPMGFTWD DKRLRRAGLD
60 70 80 90 100
YHEFAAVQRH HDYAAFVAAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG
110 120 130 140 150
PETRGLPASI LDALPKEQKI RIPMMPDSRS MNLSNAVSVV VYEAWRQLGY

PGAVLRS
Length:157
Mass (Da):17,641
Last modified:March 1, 2002 - v1
Checksum:iAD0E1D36BE19541F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA Translation: AAL22554.1
RefSeqiNP_462595.1, NC_003197.2
WP_000932335.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL22554; AAL22554; STM3695
GeneIDi1255219
KEGGistm:STM3695
PATRICifig|99287.12.peg.3908

Similar proteinsi

Entry informationi

Entry nameiQ8ZL60_SALTY
AccessioniPrimary (citable) accession number: Q8ZL60
Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2002
Last sequence update: March 1, 2002
Last modified: February 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health