Q8ZKW6 (MNMG_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	STM3874

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 629	629	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129		PRO_0000117171
Regions
	Nucleotide binding	13 – 18	6	FAD By similarity
	Nucleotide binding	273 – 287	15	NAD Potential
Sites
	Binding site	125	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	180	1	FAD By similarity
	Binding site	370	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZKW6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9B1693233DB1FDA6	FASTA	629	69,640
	10 20 30 40 50 60 MFYQDPFDVI IIGGGHAGTE AAMAAARMGQ QTLLLTHNID TLGQMSCNPA IGGIGKGHLV 70 80 90 100 110 120 KEVDALGGLM AKAIDQAGIQ FRILNASKGP AVRATRAQAD RVLYRQAVRT ALENQPNLMI 130 140 150 160 170 180 FQQAVEDLIV ENDRVVGAVT QMGLKFRAKA VVLTVGTFLD GKIHIGLDNY SGGRAGDPPS 190 200 210 220 230 240 IPLSRRLREL PLRVSRLKTG TPPRIDARTI DFSVLAQQHG DNPMPVFSFM GNASQHPQQV 250 260 270 280 290 300 PCYITHTNEK THDVIRNNLD RSPMYAGVIE GIGPRYCPSI EDKVMRFADR NQHQIFLEPE 310 320 330 340 350 360 GLTSNEIYPN GISTSLPFDV QMQIVRSMQG MENAKIVRPG YAIEYDFFDP RDLKPTLESK 370 380 390 400 410 420 FIHGLFFAGQ INGTTGYEEA AAQGLLAGLN AARLSADKEG WAPARSQAYL GVLVDDLCTL 430 440 450 460 470 480 GTKEPYRMFT SRAEYRLMLR EDNADLRLTE MGRELGLVDD ERWARFNEKL ENIERERQRL 490 500 510 520 530 540 KSTWVTPSAE SADEVNAHLT TPLSREASGE DLLRRPEMTY AQLTSLAAFA PALEDEQAAE 550 560 570 580 590 600 QVEIQVKYEG YIARQQDEIE KQLRNENTLL PATLDYRQVS GLSNEVIAKL NDHKPASIGQ 610 620 ASRISGVTPA AISILLVWLK KQGMLRRSA

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL22732.1.
RefSeq	NP_462773.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q8ZKW6.
SMR	Q8ZKW6. Positions 1-553.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM3874.
Proteomic databases
PaxDb	Q8ZKW6.
PRIDE	Q8ZKW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL22732; AAL22732; STM3874.
GeneID	1255401.
KEGG	stm:STM3874.
PATRIC	32386617. VBISalEnt20916_4104.
Phylogenomic databases
eggNOG	COG0445.
HOGENOM	HOG000201059.
KO	K03495.
OMA	AQMSCNP.
ProtClustDB	PRK05192.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA.
InterPro	IPR004416. GidA. IPR026904. GidA-assoc_3. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. PF13932. GIDA_assoc_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_SALTY

Accession

Primary (citable) accession number: Q8ZKW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program