Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8ZKW6 (MNMG_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Alternative name(s):
Glucose-inhibited division protein A
Gene names
Name:mnmG
Synonyms:gidA
Ordered Locus Names:STM3874
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length629 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129

Cofactor

FAD By similarity. HAMAP-Rule MF_00129

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP-Rule MF_00129

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00129.

Sequence similarities

Belongs to the MnmG family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
NAD
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA wobble uridine modification

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 629629tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129
PRO_0000117171

Regions

Nucleotide binding13 – 186FAD By similarity
Nucleotide binding273 – 28715NAD Potential

Sites

Binding site1251FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding site1801FAD By similarity
Binding site3701FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ZKW6 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 9B1693233DB1FDA6

FASTA62969,640
        10         20         30         40         50         60 
MFYQDPFDVI IIGGGHAGTE AAMAAARMGQ QTLLLTHNID TLGQMSCNPA IGGIGKGHLV 

        70         80         90        100        110        120 
KEVDALGGLM AKAIDQAGIQ FRILNASKGP AVRATRAQAD RVLYRQAVRT ALENQPNLMI 

       130        140        150        160        170        180 
FQQAVEDLIV ENDRVVGAVT QMGLKFRAKA VVLTVGTFLD GKIHIGLDNY SGGRAGDPPS 

       190        200        210        220        230        240 
IPLSRRLREL PLRVSRLKTG TPPRIDARTI DFSVLAQQHG DNPMPVFSFM GNASQHPQQV 

       250        260        270        280        290        300 
PCYITHTNEK THDVIRNNLD RSPMYAGVIE GIGPRYCPSI EDKVMRFADR NQHQIFLEPE 

       310        320        330        340        350        360 
GLTSNEIYPN GISTSLPFDV QMQIVRSMQG MENAKIVRPG YAIEYDFFDP RDLKPTLESK 

       370        380        390        400        410        420 
FIHGLFFAGQ INGTTGYEEA AAQGLLAGLN AARLSADKEG WAPARSQAYL GVLVDDLCTL 

       430        440        450        460        470        480 
GTKEPYRMFT SRAEYRLMLR EDNADLRLTE MGRELGLVDD ERWARFNEKL ENIERERQRL 

       490        500        510        520        530        540 
KSTWVTPSAE SADEVNAHLT TPLSREASGE DLLRRPEMTY AQLTSLAAFA PALEDEQAAE 

       550        560        570        580        590        600 
QVEIQVKYEG YIARQQDEIE KQLRNENTLL PATLDYRQVS GLSNEVIAKL NDHKPASIGQ 

       610        620 
ASRISGVTPA AISILLVWLK KQGMLRRSA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006468 Genomic DNA. Translation: AAL22732.1.
RefSeqNP_462773.1. NC_003197.1.

3D structure databases

ProteinModelPortalQ8ZKW6.
SMRQ8ZKW6. Positions 1-553.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM3874.

Proteomic databases

PaxDbQ8ZKW6.
PRIDEQ8ZKW6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL22732; AAL22732; STM3874.
GeneID1255401.
KEGGstm:STM3874.
PATRIC32386617. VBISalEnt20916_4104.

Phylogenomic databases

eggNOGCOG0445.
HOGENOMHOG000201059.
KOK03495.
OMAHTNEQTH.
OrthoDBEOG6W9X6J.
PhylomeDBQ8ZKW6.

Enzyme and pathway databases

BioCycSENT99287:GCTI-3904-MONOMER.

Family and domain databases

HAMAPMF_00129. MnmG_GidA.
InterProIPR004416. GidA.
IPR026904. GidA-assoc_3.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc_3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00136. gidA. 1 hit.
PROSITEPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMNMG_SALTY
AccessionPrimary (citable) accession number: Q8ZKW6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families