Reviewed, UniProtKB/Swiss-Prot Q8ZKW4 (ASNA_SALTY)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	STM3877

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195887

Sequences

	Sequence		Length	Mass (Da)
	Q8ZKW4-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: DB916537BC18C383	FASTA	330	36,806
	10 20 30 40 50 60 MKTAYIAKQR QISFVKSHFS RQLEERLGLI EVQAPILSRV GDGTQDNLSG CEKAVQVKVK 70 80 90 100 110 120 ALPDAQFEVV HSLAKWKRQT LGQHDFSAGE GLYTHMKALR PDEDRLSPLH SVYVDQWDWE 130 140 150 160 170 180 RVMGDGERQF STLKSTVEAI WAGIKATEAE VHKQFGLAPF LPEQIQFVHS QELLARFPDL 190 200 210 220 230 240 DAKGRERAIA KELGAVFLVG IGGKLSDGHR HDVRAPDYDD WSSASELGYA GLNGDILVWN 250 260 270 280 290 300 PVLEDAFELS SMGIRVDADT LMRQLALTGD EDRLQLEWHQ ALLRGEMPQT IGGGIGQSRL 310 320 330 TMLLLQLPHI GQVQCGVWPA QVRESIPAIL

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006468 Genomic DNA. Translation: AAL22735.1.
RefSeq	NP_462776.1.
3D structure databases
HSSP	HSSP built from PDB template 12AS based on UniProtKB P00963.
SMR	Q8ZKW4. Positions 4-330.
ModBase	Search...
Proteomic databases
PRIDE	Q8ZKW4.
Genome annotation databases
GeneID	1255404.
GenomeReviews	Gene locus STM3877 in contig AE006468_GR.
KEGG	stm:STM3877.
NMPDR	fig|99287.1.peg.3750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZKW4.
OMA	LNDNLNG.
Enzyme and pathway databases
BioCyc	STYP99287:STM3877-MON.
BRENDA	6.3.1.1. 2.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_SALTY

Accession

Primary (citable) accession number: Q8ZKW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)