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Protein

Sulfoquinovose isomerase

Gene

yihS

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of sulfoquinovose (SQ) to 6-deoxy-6-sulfo-D-fructose (SF) (By similarity). In vitro, can also catalyze the interconversion of mannose, fructose and glucose, but has extremely low activity with glucose (PubMed:18328504).UniRule annotation1 Publication

Catalytic activityi

Sulfoquinovose = 6-deoxy-6-sulfo-D-fructose.UniRule annotation

Kineticsi

kcat is 23.3 sec(-1) for D-mannose. kcat is 12.3 sec(-1) for D-fructose.1 Publication
  1. KM=134 mM for D-mannose1 Publication
  2. KM=292 mM for D-fructose1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei55SubstrateUniRule annotation1 Publication1
    Binding sitei111SubstrateUniRule annotation1 Publication1
    Binding sitei172SubstrateUniRule annotation1 Publication1
    Binding sitei176SubstrateUniRule annotation1 Publication1
    Binding sitei238Substrate; via carbonyl oxygenUniRule annotation1 Publication1
    Active sitei248Proton donor/acceptorUniRule annotation1 Publication1
    Binding sitei251SubstrateUniRule annotation1 Publication1
    Binding sitei316SubstrateUniRule annotation1 Publication1
    Active sitei383Proton donor/acceptorUniRule annotation1 Publication1

    GO - Molecular functioni

    Keywordsi

    Molecular functionIsomerase

    Enzyme and pathway databases

    BioCyciSENT99287:G1FZD-4059-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfoquinovose isomeraseUniRule annotation (EC:5.3.1.31UniRule annotation)
    Short name:
    SQ isomeraseUniRule annotation
    Gene namesi
    Name:yihS
    Ordered Locus Names:STM4021
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004291341 – 413Sulfoquinovose isomeraseAdd BLAST413

    Proteomic databases

    PaxDbiQ8ZKT7

    Interactioni

    Subunit structurei

    Homohexamer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM4021

    Structurei

    Secondary structure

    1413
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi7 – 24Combined sources18
    Helixi25 – 27Combined sources3
    Helixi44 – 46Combined sources3
    Helixi50 – 65Combined sources16
    Helixi71 – 82Combined sources12
    Turni83 – 86Combined sources4
    Turni89 – 91Combined sources3
    Beta strandi92 – 94Combined sources3
    Beta strandi96 – 99Combined sources4
    Beta strandi102 – 105Combined sources4
    Helixi110 – 124Combined sources15
    Turni125 – 127Combined sources3
    Helixi131 – 145Combined sources15
    Turni149 – 152Combined sources4
    Helixi171 – 186Combined sources16
    Helixi192 – 204Combined sources13
    Helixi205 – 209Combined sources5
    Helixi210 – 212Combined sources3
    Turni228 – 233Combined sources6
    Beta strandi238 – 240Combined sources3
    Beta strandi242 – 244Combined sources3
    Helixi246 – 265Combined sources20
    Helixi273 – 289Combined sources17
    Beta strandi293 – 298Combined sources6
    Beta strandi308 – 310Combined sources3
    Helixi315 – 332Combined sources18
    Helixi335 – 351Combined sources17
    Turni355 – 357Combined sources3
    Beta strandi358 – 360Combined sources3
    Beta strandi362 – 364Combined sources3
    Helixi382 – 384Combined sources3
    Helixi385 – 388Combined sources4
    Helixi390 – 392Combined sources3
    Beta strandi395 – 397Combined sources3
    Helixi399 – 404Combined sources6
    Turni408 – 411Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2AFAX-ray2.15A/B/C/D/E/F2-413[»]
    2ZBLX-ray1.60A/B/C/D/E/F1-413[»]
    ProteinModelPortaliQ8ZKT7
    SMRiQ8ZKT7
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8ZKT7

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N-acylglucosamine 2-epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4107QKS Bacteria
    COG2942 LUCA
    HOGENOMiHOG000244284
    KOiK18479
    OMAiWVQAETF
    PhylomeDBiQ8ZKT7

    Family and domain databases

    CDDicd00249 AGE, 1 hit
    Gene3Di1.50.10.10, 1 hit
    HAMAPiMF_00998 SQ_isomerase, 1 hit
    InterProiView protein in InterPro
    IPR008928 6-hairpin_glycosidase_sf
    IPR012341 6hp_glycosidase-like_sf
    IPR034116 AGE_dom
    IPR030875 SQ_isomerase
    SUPFAMiSSF48208 SSF48208, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q8ZKT7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKWFNTLSHN RWLEQETDRI FNFGKNAVVP TGFGWLGNKG QIKEEMGTHL
    60 70 80 90 100
    WITARMLHVY SVAASMGRPG AYDLVDHGIK AMNGALRDKK YGGWYACVND
    110 120 130 140 150
    QGVVDASKQG YQHFFALLGA ASAVTTGHPE ARKLLDYTIE VIEKYFWSEE
    160 170 180 190 200
    EQMCLESWDE AFSQTEDYRG GNANMHAVEA FLIVYDVTHD KKWLDRALRI
    210 220 230 240 250
    ASVIIHDVAR NGDYRVNEHF DSQWNPIRDY NKDNPAHRFR AYGGTPGHWI
    260 270 280 290 300
    EWGRLMLHLH AALEARFETP PAWLLEDAKG LFHATIRDAW APDGADGFVY
    310 320 330 340 350
    SVDWDGKPIV RERVRWPIVE AMGTAYALYT LTDDSQYEEW YQKWWDYCIK
    360 370 380 390 400
    YLMDYENGSW WQELDADNKV TTKVWDGKQD IYHLLHCLVI PRLPLAPGLA
    410
    PAVAAGLLDI NAK
    Length:413
    Mass (Da):47,527
    Last modified:March 1, 2002 - v1
    Checksum:i030CB11DC3A1A2E3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA Translation: AAL22860.1
    RefSeqiNP_462901.1, NC_003197.2
    WP_000870946.1, NC_003197.2

    Genome annotation databases

    EnsemblBacteriaiAAL22860; AAL22860; STM4021
    GeneIDi1255547
    KEGGistm:STM4021
    PATRICifig|99287.12.peg.4236

    Similar proteinsi

    Entry informationi

    Entry nameiSQUS_SALTY
    AccessioniPrimary (citable) accession number: Q8ZKT7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: March 1, 2002
    Last modified: March 28, 2018
    This is version 98 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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