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Protein

Sulfoquinovose isomerase

Gene

yihS

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of sulfoquinovose (SQ) to 6-deoxy-6-sulfo-D-fructose (SF) (By similarity). In vitro, can also catalyze the interconversion of mannose, fructose and glucose, but has extremely low activity with glucose (PubMed:18328504).UniRule annotation1 Publication

Catalytic activityi

Sulfoquinovose = 6-deoxy-6-sulfo-D-fructose.UniRule annotation

Kineticsi

kcat is 23.3 sec(-1) for D-mannose. kcat is 12.3 sec(-1) for D-fructose.1 Publication

  1. KM=134 mM for D-mannose1 Publication
  2. KM=292 mM for D-fructose1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei55 – 551SubstrateUniRule annotation1 Publication
    Binding sitei111 – 1111SubstrateUniRule annotation1 Publication
    Binding sitei172 – 1721SubstrateUniRule annotation1 Publication
    Binding sitei176 – 1761SubstrateUniRule annotation1 Publication
    Binding sitei238 – 2381Substrate; via carbonyl oxygenUniRule annotation1 Publication
    Active sitei248 – 2481Proton donor/acceptorUniRule annotation1 Publication
    Binding sitei251 – 2511SubstrateUniRule annotation1 Publication
    Binding sitei316 – 3161SubstrateUniRule annotation1 Publication
    Active sitei383 – 3831Proton donor/acceptorUniRule annotation1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-4049-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfoquinovose isomeraseUniRule annotation (EC:5.3.1.31UniRule annotation)
    Short name:
    SQ isomeraseUniRule annotation
    Gene namesi
    Name:yihS
    Ordered Locus Names:STM4021
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 413413Sulfoquinovose isomerasePRO_0000429134Add
    BLAST

    Proteomic databases

    PaxDbiQ8ZKT7.

    Interactioni

    Subunit structurei

    Homohexamer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM4021.

    Structurei

    Secondary structure

    1
    413
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 2418Combined sources
    Helixi25 – 273Combined sources
    Helixi44 – 463Combined sources
    Helixi50 – 6516Combined sources
    Helixi71 – 8212Combined sources
    Turni83 – 864Combined sources
    Turni89 – 913Combined sources
    Beta strandi92 – 943Combined sources
    Beta strandi96 – 994Combined sources
    Beta strandi102 – 1054Combined sources
    Helixi110 – 12415Combined sources
    Turni125 – 1273Combined sources
    Helixi131 – 14515Combined sources
    Turni149 – 1524Combined sources
    Helixi171 – 18616Combined sources
    Helixi192 – 20413Combined sources
    Helixi205 – 2095Combined sources
    Helixi210 – 2123Combined sources
    Turni228 – 2336Combined sources
    Beta strandi238 – 2403Combined sources
    Beta strandi242 – 2443Combined sources
    Helixi246 – 26520Combined sources
    Helixi273 – 28917Combined sources
    Beta strandi293 – 2986Combined sources
    Beta strandi308 – 3103Combined sources
    Helixi315 – 33218Combined sources
    Helixi335 – 35117Combined sources
    Turni355 – 3573Combined sources
    Beta strandi358 – 3603Combined sources
    Beta strandi362 – 3643Combined sources
    Helixi382 – 3843Combined sources
    Helixi385 – 3884Combined sources
    Helixi390 – 3923Combined sources
    Beta strandi395 – 3973Combined sources
    Helixi399 – 4046Combined sources
    Turni408 – 4114Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AFAX-ray2.15A/B/C/D/E/F2-413[»]
    2ZBLX-ray1.60A/B/C/D/E/F1-413[»]
    ProteinModelPortaliQ8ZKT7.
    SMRiQ8ZKT7. Positions 1-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8ZKT7.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N-acylglucosamine 2-epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4107QKS. Bacteria.
    COG2942. LUCA.
    HOGENOMiHOG000244284.
    KOiK18479.
    OMAiANMHMTE.
    PhylomeDBiQ8ZKT7.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    HAMAPiMF_00998. SQ_isomerase. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR030875. SQ_isomerase.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8ZKT7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKWFNTLSHN RWLEQETDRI FNFGKNAVVP TGFGWLGNKG QIKEEMGTHL
    60 70 80 90 100
    WITARMLHVY SVAASMGRPG AYDLVDHGIK AMNGALRDKK YGGWYACVND
    110 120 130 140 150
    QGVVDASKQG YQHFFALLGA ASAVTTGHPE ARKLLDYTIE VIEKYFWSEE
    160 170 180 190 200
    EQMCLESWDE AFSQTEDYRG GNANMHAVEA FLIVYDVTHD KKWLDRALRI
    210 220 230 240 250
    ASVIIHDVAR NGDYRVNEHF DSQWNPIRDY NKDNPAHRFR AYGGTPGHWI
    260 270 280 290 300
    EWGRLMLHLH AALEARFETP PAWLLEDAKG LFHATIRDAW APDGADGFVY
    310 320 330 340 350
    SVDWDGKPIV RERVRWPIVE AMGTAYALYT LTDDSQYEEW YQKWWDYCIK
    360 370 380 390 400
    YLMDYENGSW WQELDADNKV TTKVWDGKQD IYHLLHCLVI PRLPLAPGLA
    410
    PAVAAGLLDI NAK
    Length:413
    Mass (Da):47,527
    Last modified:March 1, 2002 - v1
    Checksum:i030CB11DC3A1A2E3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL22860.1.
    RefSeqiNP_462901.1. NC_003197.1.
    WP_000870946.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL22860; AAL22860; STM4021.
    GeneIDi1255547.
    KEGGistm:STM4021.
    PATRICi32386912. VBISalEnt20916_4236.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL22860.1.
    RefSeqiNP_462901.1. NC_003197.1.
    WP_000870946.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AFAX-ray2.15A/B/C/D/E/F2-413[»]
    2ZBLX-ray1.60A/B/C/D/E/F1-413[»]
    ProteinModelPortaliQ8ZKT7.
    SMRiQ8ZKT7. Positions 1-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi99287.STM4021.

    Proteomic databases

    PaxDbiQ8ZKT7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL22860; AAL22860; STM4021.
    GeneIDi1255547.
    KEGGistm:STM4021.
    PATRICi32386912. VBISalEnt20916_4236.

    Phylogenomic databases

    eggNOGiENOG4107QKS. Bacteria.
    COG2942. LUCA.
    HOGENOMiHOG000244284.
    KOiK18479.
    OMAiANMHMTE.
    PhylomeDBiQ8ZKT7.

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-4049-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiQ8ZKT7.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    HAMAPiMF_00998. SQ_isomerase. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR030875. SQ_isomerase.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSQUS_SALTY
    AccessioniPrimary (citable) accession number: Q8ZKT7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: March 1, 2002
    Last modified: September 7, 2016
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.