Q8ZKM0 (CAPP_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppc
Ordered Locus Names:	STM4119

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	883 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_00595
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595
Cofactor	Magnesium By similarity.
Enzyme regulation	The enzyme has distinct binding sites for each of the allosteric effectors such as acetyl-CoA, fructose 1,6-bisphosphate, guanosine 3'-diphosphate 5'-diphosphate, long chain fatty acids, and L-aspartate By similarity. HAMAP-Rule MF_00595
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: HAMAP oxaloacetate metabolic process Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 883	883	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00595		PRO_0000166622
Sites
	Active site	138	1	By similarity
	Active site	546	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZKM0 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 2F28CE22308A003E	FASTA	883	99,003
	10 20 30 40 50 60 MNEQYSALRS NVSMLGKVLG ETIKDALGEH ILDRVETIRK LSKSSRAGNE ANRQELLTTL 70 80 90 100 110 120 QNLSNDELLP VARAFSQFLN LANTAEQYHS ISPKGEAASN PEVIARTLRK LKNQPDLNDA 130 140 150 160 170 180 TIKKAVESLS LELVLTAHPT EITRRTLIHK MGEINNCLKQ LDNTDIADYE RHQVMRRLRQ 190 200 210 220 230 240 LIAQSWHTDE IRKQRPSPVD EAKWGFAVVE NSLWQGVPNY LRELNEQLEE NLGYKLPVDF 250 260 270 280 290 300 VPVRFTSWMG GDRDGNPNVT ADITRHVLLL SRWKATDLFL KDIHVLVSEL SMVDATPELL 310 320 330 340 350 360 ALVGEEGASE PYRYLMKKLR ARLMATQSWL EARLKGEKLP KPAGLLTQNE QLWEPLYACY 370 380 390 400 410 420 QSLQACGMGI IANGELLDTL RRVKCFGVPL VRIDIRQEST RHTEALGEIT RYLGIGDYES 430 440 450 460 470 480 WSEADKQAFL IRELNSKRPL LPRNWEPSND TREVLETCKV IAEAPKGSIA AYVISMAKTP 490 500 510 520 530 540 SDVLAVHLLL KEAGIGFAMP VAPLFETLDD LNNADDVMTQ LLNIDWYRGL IQGKQMVMIG 550 560 570 580 590 600 YSDSAKDAGV MAASWAQYQA QDALIKTCEK AGIELTLFHG RGGSIGRGGA PAHAALLSQP 610 620 630 640 650 660 PGSLKGGLRV TEQGEMIRFK YGLPEVTVSS LSLYTSAILE ANLLPPPEPK DSWRHIMDEL 670 680 690 700 710 720 SVISCETYRG YVRENKDFVP YFRSATPEQE LGKLPLGSRP AKRRPTGGVE SLRAIPWIFA 730 740 750 760 770 780 WTQNRLMLPA WLGAGTALQK VVEDGKQSEL EAMCRDWPFF STRLGMLEMV FSKADLWLAD 790 800 810 820 830 840 YYDQRLVAKT LWPLGKELRD LLEEDIKVVL AIANDSHLMA DLPWIAESIQ LRNVYTDPLN 850 860 870 880 VLQAELLYRS RLTEEQGKSP DPRVEQALMV TIAGVAAGMR NTG

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL22958.1.
RefSeq	NP_462999.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q8ZKM0.
SMR	Q8ZKM0. Positions 4-883.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM4119.
Proteomic databases
PaxDb	Q8ZKM0.
PRIDE	Q8ZKM0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL22958; AAL22958; STM4119.
GeneID	1255646.
KEGG	stm:STM4119.
PATRIC	32387122. VBISalEnt20916_4341.
Phylogenomic databases
eggNOG	COG2352.
HOGENOM	HOG000238648.
KO	K01595.
OMA	AIPWVFG.
ProtClustDB	PRK00009.
Family and domain databases
HAMAP	MF_00595. PEPcase_type1.
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP_SALTY

Accession

Primary (citable) accession number: Q8ZKM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program