Reviewed, UniProtKB/Swiss-Prot Q8ZK57 (IOLG_SALTY)

Names and origin

Protein names

Recommended name:
    Inositol 2-dehydrogenase
    EC=1.1.1.18
Alternative name(s):
    Myo-inositol 2-dehydrogenase
      Short name=MI 2-dehydrogenase

Gene names

Name:	iolG
Ordered Locus Names:	STM4425

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the oxidation of myo-inositol (MI) to 2-keto-myo-inositol (2KMI or 2-inosose) By similarity.
Catalytic activity	Myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH. HAMAP MF_01671
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the gfo/idh/mocA family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro inositol 2-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 336

336

Inositol 2-dehydrogenase HAMAP MF_01671

PRO_0000352595

Secondary structure

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336

Helix Strand Turn

Details...

Helix

16 – 23

8

Beta strand

32 – 34

3

Helix

41 – 48

8

Beta strand

53 – 57

5

Helix

58 – 63

6

Beta strand

69 – 72

4

Helix

76 – 78

3

Helix

79 – 88

10

Beta strand

92 – 95

4

Beta strand

97 – 99

3

Helix

103 – 115

13

Helix

126 – 129

4

Helix

134 – 141

8

Beta strand

150 – 152

3

Beta strand

154 – 156

3

Helix

168 – 171

4

Helix

174 – 185

12

Beta strand

189 – 195

7

Beta strand

209 – 216

8

Beta strand

221 – 227

7

Beta strand

235 – 241

7

Beta strand

256 – 259

4

Beta strand

262 – 266

5

Helix

271 – 273

3

Helix

276 – 291

16

Helix

306 – 319

14

Helix

333 – 335

3

Sequences

	Sequence		Length	Mass (Da)
	Q8ZK57-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 5E66D7BC40158197	FASTA	336	37,267
	10 20 30 40 50 60 MTLKAGIVGI GMIGSDHLRR LANTVSGVEV VAVCDIVAGR AQAALDKYAI EAKDYNDYHD 70 80 90 100 110 120 LINDKDVEVV IITASNEAHA DVAVAALNAN KYVFCEKPLA VTAADCQRVI EAEQKNGKRM 130 140 150 160 170 180 VQIGFMRRYD KGYVQLKNII DSGEIGQPLM VHGRHYNAST VPEYKTPQAI YETLIHEIDV 190 200 210 220 230 240 MHWLLNEDYK TVKVYFPRQS SLVTTLRDPQ LVVMETTSGI NIVVEVFVNC QYGYDIHCDV 250 260 270 280 290 300 TGEKGMAELP TVASAAVRKA AKYSTDILVD WKQRFIDAYD IEFQDFFDRL NAGLPPAGPT 310 320 330 SWDGYLAAVT ADACVKSQET GNTEIVELPS KPDFYK

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE006468 Genomic DNA. Translation: AAL23245.1.

RefSeq

NP_463286.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EC7	X-ray	2.15	A/B/C/D/E/F/G/H	1-336	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q8ZK57.

Genome annotation databases

GeneID

1255951.

GenomeReviews

Gene locus STM4425 in contig AE006468_GR.

KEGG

stm:STM4425.

NMPDR

fig|99287.1.peg.4258.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q8ZK57.

OMA

DWKDRFI.

Enzyme and pathway databases

BioCyc

STYP99287:STM4425-MONOMER.

Family and domain databases

HAMAP

MF_01671.
[Tree]

InterPro

IPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR004104. OxRdtase_C.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF01408. GFO_IDH_MocA. 1 hit.
PF02894. GFO_IDH_MocA_C. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IOLG_SALTY

Accession

Primary (citable) accession number: Q8ZK57

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)