Q8ZJL6 (K6PF_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	YPO0078, y0059, YP_0080

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_0000112009
Regions
	Nucleotide binding	22 – 26	5	ATP By similarity
	Nucleotide binding	156 – 160	5	ATP By similarity
	Nucleotide binding	173 – 189	17	ATP By similarity
Sites
	Active site	129	1	Proton acceptor By similarity
	Metal binding	187	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	189	1	Magnesium By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZJL6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1D54B3BABD0E430D	FASTA	327	35,395
	10 20 30 40 50 60 MVKKIGVLTS GGDAPGMNAA IRGVVRAALS AGLDVFGIED GYLGLYENRM KKLDRYSVSD 70 80 90 100 110 120 MINRGGTFLG SARFPEFRDP EVRKVALKNM HERGIDGLVV IGGDGSYAGA DLLTKEGGIH 130 140 150 160 170 180 CVGLPGTIDN DVAGTDYTIG FFTALETVVE AIDRLRDTSS SHQRISIVEV MGRYCGDLTL 190 200 210 220 230 240 AAAIAGGCEF IAIPEVEFKR DDLVAEIKAG IAKGKKHAIV AITEKLDDID SLAKYIEKET 250 260 270 280 290 300 GRETRGTVLG HIQRGGAPVA YDRILASRMG AYAVDLLLQD HDYKKGGFCV GVQNEKMVHE 310 320 LISVCIAPEN KKSKFKEDWY DTAKKLF

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL18767.1. AE009952 Genomic DNA. Translation: AAM83654.1. AE017042 Genomic DNA. Translation: AAS60360.1.
PIR	AF0010.
RefSeq	NP_667403.1. NC_004088.1. NP_991483.1. NC_005810.1. YP_002345172.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZJL6.
SMR	Q8ZJL6. Positions 1-327.
ModBase	Search...
Protein-protein interaction databases
STRING	214092.YPO0078.
Proteomic databases
PRIDE	Q8ZJL6.
Protocols and materials databases
DNASU	1145006.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM83654; AAM83654; y0059. AAS60360; AAS60360; YP_0080.
GeneID	1145006. 1172927. 2766904.
KEGG	ype:YPO0078. ypk:y0059. ypm:YP_0080.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248870.
KO	K00850.
OMA	EFKTVEG.
ProtClustDB	PRK03202.
Enzyme and pathway databases
BioCyc	YPES214092:GKDD-82-MONOMER.
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_YERPE

Accession

Primary (citable) accession number: Q8ZJL6
Secondary accession number(s): Q0WKL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program