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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei206 – 2061ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:YPO0157, y3940, YP_0159
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Tryptophan--tRNA ligasePRO_0000136716Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y3940.

Structurei

Secondary structure

1
342
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 186Combined sources
Helixi26 – 316Combined sources
Helixi33 – 375Combined sources
Turni38 – 425Combined sources
Beta strandi43 – 497Combined sources
Helixi51 – 544Combined sources
Helixi61 – 7818Combined sources
Turni82 – 843Combined sources
Beta strandi85 – 895Combined sources
Helixi90 – 923Combined sources
Helixi95 – 10410Combined sources
Helixi109 – 1135Combined sources
Helixi116 – 1249Combined sources
Helixi126 – 1283Combined sources
Helixi131 – 14515Combined sources
Turni146 – 1483Combined sources
Beta strandi150 – 1523Combined sources
Helixi156 – 1583Combined sources
Helixi159 – 17618Combined sources
Helixi211 – 2133Combined sources
Helixi221 – 2299Combined sources
Turni245 – 2473Combined sources
Helixi249 – 26214Combined sources
Helixi266 – 2727Combined sources
Turni273 – 2753Combined sources
Helixi278 – 30427Combined sources
Helixi307 – 33630Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N9IX-ray1.95A/B1-342[»]
ProteinModelPortaliQ8ZJF2.
SMRiQ8ZJF2. Positions 13-339.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZJF2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi20 – 289"HIGH" region
Motifi203 – 2075"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31. Bacteria.
COG0180. LUCA.
HOGENOMiHOG000059940.
KOiK01867.
OMAiGWGQFKP.
OrthoDBiEOG686NJQ.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZJF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSKPTVSS KPIVFSGAQP SGELTIGNYM GALRQWVQMQ DDYDCIYCIV
60 70 80 90 100
DLHAITARQD PALLRKRTLD TLALYLACGI DPKKSTIFVQ SHVPEHSQLS
110 120 130 140 150
WALNCYTYFG ELSRMTQFKD KSARYAENIN AGLFDYPVLM AADILLYQTN
160 170 180 190 200
QVPVGEDQKQ HLELSRDIAS RFNNLYGDIF KIPEPFIPKA GARVMSLQDP
210 220 230 240 250
TKKMSKSDDN RNNVIELLED PKSVVKKIKR AMTDSDEPAL IRYDVEKKAG
260 270 280 290 300
VSNLLDILSG VTGQSIPELE AQFTGQMYGH LKGAVADAVS GMLSELQERY
310 320 330 340
RTYREDEALL QDVMREGAAK ARARAQVTLA KVYEAIGFVA QP
Length:342
Mass (Da):38,225
Last modified:November 8, 2002 - v2
Checksum:i79B4EDAEC31E0745
GO

Sequence cautioni

The sequence AAM87484.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAS60437.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAL18843.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL18843.1. Different initiation.
AE009952 Genomic DNA. Translation: AAM87484.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS60437.1. Different initiation.
PIRiAB0020.
RefSeqiYP_002345243.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM87484; AAM87484; y3940.
AAS60437; AAS60437; YP_0159.
GeneIDi1173003.
KEGGiype:YPO0157.
ypk:y3940.
ypm:YP_0159.
PATRICi18589337. VBIYerPes7843_0408.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL18843.1. Different initiation.
AE009952 Genomic DNA. Translation: AAM87484.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS60437.1. Different initiation.
PIRiAB0020.
RefSeqiYP_002345243.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N9IX-ray1.95A/B1-342[»]
ProteinModelPortaliQ8ZJF2.
SMRiQ8ZJF2. Positions 13-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187410.y3940.

Protocols and materials databases

DNASUi1148887.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM87484; AAM87484; y3940.
AAS60437; AAS60437; YP_0159.
GeneIDi1173003.
KEGGiype:YPO0157.
ypk:y3940.
ypm:YP_0159.
PATRICi18589337. VBIYerPes7843_0408.

Phylogenomic databases

eggNOGiENOG4105C31. Bacteria.
COG0180. LUCA.
HOGENOMiHOG000059940.
KOiK01867.
OMAiGWGQFKP.
OrthoDBiEOG686NJQ.

Enzyme and pathway databases

BioCyciYPES214092:GKDD-157-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8ZJF2.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiSYW_YERPE
AccessioniPrimary (citable) accession number: Q8ZJF2
Secondary accession number(s): Q0WKE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: November 11, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.