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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei206ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:YPO0157, y3940, YP_0159
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367161 – 342Tryptophan--tRNA ligaseAdd BLAST342

Proteomic databases

PRIDEiQ8ZJF2.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y3940.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 18Combined sources6
Helixi26 – 31Combined sources6
Helixi33 – 37Combined sources5
Turni38 – 42Combined sources5
Beta strandi43 – 49Combined sources7
Helixi51 – 54Combined sources4
Helixi61 – 78Combined sources18
Turni82 – 84Combined sources3
Beta strandi85 – 89Combined sources5
Helixi90 – 92Combined sources3
Helixi95 – 104Combined sources10
Helixi109 – 113Combined sources5
Helixi116 – 124Combined sources9
Helixi126 – 128Combined sources3
Helixi131 – 145Combined sources15
Turni146 – 148Combined sources3
Beta strandi150 – 152Combined sources3
Helixi156 – 158Combined sources3
Helixi159 – 176Combined sources18
Helixi211 – 213Combined sources3
Helixi221 – 229Combined sources9
Turni245 – 247Combined sources3
Helixi249 – 262Combined sources14
Helixi266 – 272Combined sources7
Turni273 – 275Combined sources3
Helixi278 – 304Combined sources27
Helixi307 – 336Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N9IX-ray1.95A/B1-342[»]
ProteinModelPortaliQ8ZJF2.
SMRiQ8ZJF2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZJF2.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 28"HIGH" region9
Motifi203 – 207"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31. Bacteria.
COG0180. LUCA.
HOGENOMiHOG000059940.
KOiK01867.
OMAiGWGQFKP.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZJF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSKPTVSS KPIVFSGAQP SGELTIGNYM GALRQWVQMQ DDYDCIYCIV
60 70 80 90 100
DLHAITARQD PALLRKRTLD TLALYLACGI DPKKSTIFVQ SHVPEHSQLS
110 120 130 140 150
WALNCYTYFG ELSRMTQFKD KSARYAENIN AGLFDYPVLM AADILLYQTN
160 170 180 190 200
QVPVGEDQKQ HLELSRDIAS RFNNLYGDIF KIPEPFIPKA GARVMSLQDP
210 220 230 240 250
TKKMSKSDDN RNNVIELLED PKSVVKKIKR AMTDSDEPAL IRYDVEKKAG
260 270 280 290 300
VSNLLDILSG VTGQSIPELE AQFTGQMYGH LKGAVADAVS GMLSELQERY
310 320 330 340
RTYREDEALL QDVMREGAAK ARARAQVTLA KVYEAIGFVA QP
Length:342
Mass (Da):38,225
Last modified:November 8, 2002 - v2
Checksum:i79B4EDAEC31E0745
GO

Sequence cautioni

The sequence AAM87484 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS60437 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAL18843 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL18843.1. Different initiation.
AE009952 Genomic DNA. Translation: AAM87484.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS60437.1. Different initiation.
PIRiAB0020.
RefSeqiYP_002345243.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM87484; AAM87484; y3940.
AAS60437; AAS60437; YP_0159.
GeneIDi1173003.
KEGGiype:YPO0157.
ypk:y3940.
ypm:YP_0159.
PATRICi18589337. VBIYerPes7843_0408.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL18843.1. Different initiation.
AE009952 Genomic DNA. Translation: AAM87484.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS60437.1. Different initiation.
PIRiAB0020.
RefSeqiYP_002345243.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N9IX-ray1.95A/B1-342[»]
ProteinModelPortaliQ8ZJF2.
SMRiQ8ZJF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187410.y3940.

Proteomic databases

PRIDEiQ8ZJF2.

Protocols and materials databases

DNASUi1148887.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM87484; AAM87484; y3940.
AAS60437; AAS60437; YP_0159.
GeneIDi1173003.
KEGGiype:YPO0157.
ypk:y3940.
ypm:YP_0159.
PATRICi18589337. VBIYerPes7843_0408.

Phylogenomic databases

eggNOGiENOG4105C31. Bacteria.
COG0180. LUCA.
HOGENOMiHOG000059940.
KOiK01867.
OMAiGWGQFKP.

Miscellaneous databases

EvolutionaryTraceiQ8ZJF2.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYW_YERPE
AccessioniPrimary (citable) accession number: Q8ZJF2
Secondary accession number(s): Q0WKE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: November 30, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.