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Q8ZIM0

- SYI_YERPE

UniProt

Q8ZIM0 - SYI_YERPE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Yersinia pestis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciYPES214092:GKDD-470-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:YPO0475, y3700, YP_3705
    OrganismiYersinia pestis
    Taxonomic identifieri632 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_0000098511Add
    BLAST

    Proteomic databases

    PRIDEiQ8ZIM0.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    IntActiQ8ZIM0. 4 interactions.
    STRINGi214092.YPO0475.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8ZIM0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8ZIM0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKNTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRAAKKGKKT    50
    FILHDGPPYA NGNIHIGHSV NKILKDIIVK SKGMAGYDSP YIPGWDCHGL 100
    PIELKVEQLI GKPGEKVSAA EFRTACRKYA AEQVEGQKKD FIRLGVLGDW 150
    DHPYLTMDFK TEANIIRALS KIIDNGHLHK GAKPVHWCTD CGSSLAEAEV 200
    EYYDKTSQSI DVRFNAVDTA TVAAKFGVSA VNGPISLVIW TTTPWTLPAN 250
    RAISLNAEYL YQLVQVEGEC LILAADLVES VMKRAGITQW AVLGSCTGSD 300
    LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDFVIGQKYG 350
    LEVANPVGPN GCYLAGTYPT LDGLFVFKAN DVVVELLREK GALLHVEKLL 400
    HSYPCCWRHK TPIIFRATPQ WFISMDQKGL RKQSLQEIKG VQWIPDWGQA 450
    RIETMVANRP DWCISRQRTW GVPMSLFVHK ETEQLHPRSI ELMEEVAKRV 500
    EQDGIQAWWD LDPAEILGAD AADYVKVPDT LDVWFDSGST HSSVVDVRPE 550
    FGGHSPDMYL EGSDQHRGWF MSSLMIATAM KGKAPYRQVL THGFTVDGQG 600
    RKMSKSIGNT ISPQDVMNKL GGDILRLWVA STDYTGEIAV SDEILKRSAD 650
    SYRRIRNTAR FLLANLNGFD PAQHQVKPEE MVVVDRWAVG RAQAAQAEIM 700
    EAYENYDFHL VVQRLMQFCS VEMGSFYLDI IKDRQYTAKG DGIARRSCQT 750
    ALFHIAEALV RWMAPIMSFT ADEIWNHLPG ERQQYVFTEE WYDGLFGLAG 800
    NESMNDTFWA ELLKVRGEVN KVLEQARSDK RIGGSLEAAV TLYAEPELAA 850
    RLNSLQDELR FVLLTSAAKV AAYADAGNDA QQSELIAGLK ITFNKADGEK 900
    CPRCWHYTQD VGLVAEHAEL CGRCVTNVAG DGEERKFA 938
    Length:938
    Mass (Da):104,690
    Last modified:March 1, 2002 - v1
    Checksum:i827A66208EE0B84A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590842 Genomic DNA. Translation: CAL19154.1.
    AE009952 Genomic DNA. Translation: AAM87248.1.
    AE017042 Genomic DNA. Translation: AAS63853.1.
    PIRiAH0058.
    RefSeqiNP_670997.1. NC_004088.1.
    NP_994976.1. NC_005810.1.
    YP_002345547.1. NC_003143.1.

    Genome annotation databases

    EnsemblBacteriaiAAM87248; AAM87248; y3700.
    AAS63853; AAS63853; YP_3705.
    GeneIDi1148647.
    1173319.
    2765359.
    KEGGiype:YPO0475.
    ypk:y3700.
    ypm:YP_3705.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590842 Genomic DNA. Translation: CAL19154.1 .
    AE009952 Genomic DNA. Translation: AAM87248.1 .
    AE017042 Genomic DNA. Translation: AAS63853.1 .
    PIRi AH0058.
    RefSeqi NP_670997.1. NC_004088.1.
    NP_994976.1. NC_005810.1.
    YP_002345547.1. NC_003143.1.

    3D structure databases

    ProteinModelPortali Q8ZIM0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8ZIM0. 4 interactions.
    STRINGi 214092.YPO0475.

    Proteomic databases

    PRIDEi Q8ZIM0.

    Protocols and materials databases

    DNASUi 1148647.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM87248 ; AAM87248 ; y3700 .
    AAS63853 ; AAS63853 ; YP_3705 .
    GeneIDi 1148647.
    1173319.
    2765359.
    KEGGi ype:YPO0475.
    ypk:y3700.
    ypm:YP_3705.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci YPES214092:GKDD-470-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CO-92 / Biovar Orientalis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KIM10+ / Biovar Mediaevalis.
    3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
      Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
      , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
      DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 91001 / Biovar Mediaevalis.

    Entry informationi

    Entry nameiSYI_YERPE
    AccessioniPrimary (citable) accession number: Q8ZIM0
    Secondary accession number(s): Q0WJJ1, Q74Q17, Q7CG80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3