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Q8ZIG8 (LEU1_YERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-isopropylmalate synthase

EC=2.3.3.13
Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase
Gene names
Name:leuA
Ordered Locus Names:YPO0533, y3645, YP_3649
OrganismYersinia pestis [Reference proteome] [HAMAP]
Taxonomic identifier632 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_01025

Catalytic activity

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_01025

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_01025

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01025

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Branched-chain amino acid biosynthesis
Leucine biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function2-isopropylmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5205202-isopropylmalate synthase HAMAP-Rule MF_01025
PRO_0000140404

Experimental info

Sequence conflict1761T → P in AAM87193. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8ZIG8 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 7FC05F2FEEC0A472

FASTA52057,375
        10         20         30         40         50         60 
MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DIMEVGFPVS SPGDFESVRT 

        70         80         90        100        110        120 
IAQQVKNSRV CALARCVDKD IDVAAEALRI AEAFRIHVFL ATSTLHIESK LKRSFDDVLA 

       130        140        150        160        170        180 
MAVHSVKRAR NYTDDVEFSC EDAGRTPIDN LCRVVEAAIT AGATTINIPD TVGYTTPYQF 

       190        200        210        220        230        240 
GGIITDLYER VPNIDKAIIS VHCHDDLGMS VANSITAVQA GARQVEGTIN GLGERAGNCS 

       250        260        270        280        290        300 
LEEVIMAIKV RHEMLGVHTN INHQEIYRTS QLVSKICNMP IPGNKAIVGS NAFAHSSGIH 

       310        320        330        340        350        360 
QDGVLKNREN YEIMTPESIG LKEVQLNLTS RSGRAAVKHR MEEMGYQDKD YNLDSLYDAF 

       370        380        390        400        410        420 
LKLADKKGQV FDYDLEALAF INKQQEEPEY YRLDYFSVQS GSSVMATASV KLVCGEEIKS 

       430        440        450        460        470        480 
EAATGNGPVD AVYQAINRIT DYPIELVKYQ LSAKGQGKDA LGQVDIVVDH KGRRFHGVGL 

       490        500        510        520 
ATDIVESSAK ALVHVLNNIW RAHQVEKEKQ RLQQNNQEMV 

« Hide

References

[1]"Genome sequence of Yersinia pestis, the causative agent of plague."
Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. expand/collapse author list , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.
Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CO-92 / Biovar Orientalis.
[2]"Genome sequence of Yersinia pestis KIM."
Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. expand/collapse author list , Matson J.S., Blattner F.R., Perry R.D.
J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KIM10+ / Biovar Mediaevalis.
[3]"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. expand/collapse author list , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590842 Genomic DNA. Translation: CAL19213.1.
AE009952 Genomic DNA. Translation: AAM87193.1.
AE017042 Genomic DNA. Translation: AAS63797.1.
PIRAC0066.
RefSeqNP_670942.1. NC_004088.1.
NP_994920.1. NC_005810.1.
YP_002345605.1. NC_003143.1.

3D structure databases

ProteinModelPortalQ8ZIG8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING214092.YPO0533.

Proteomic databases

PRIDEQ8ZIG8.

Protocols and materials databases

DNASU1148592.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM87193; AAM87193; y3645.
AAS63797; AAS63797; YP_3649.
GeneID1148592.
1173378.
2766097.
KEGGype:YPO0533.
ypk:y3645.
ypm:YP_3649.

Phylogenomic databases

eggNOGCOG0119.
HOGENOMHOG000046859.
KOK01649.
OMAEGQNALG.
OrthoDBEOG6CGCF3.

Enzyme and pathway databases

BioCycYPES214092:GKDD-528-MONOMER.
UniPathwayUPA00048; UER00070.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01025. LeuA_type1.
InterProIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMSSF110921. SSF110921. 1 hit.
TIGRFAMsTIGR00973. leuA_bact. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU1_YERPE
AccessionPrimary (citable) accession number: Q8ZIG8
Secondary accession number(s): Q0WJD3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways