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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathway: L-leucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciYPES214092:GKDD-528-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:YPO0533, y3645, YP_3649
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815 Componenti: Chromosome UP000002490 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5205202-isopropylmalate synthasePRO_0000140404Add
BLAST

Proteomic databases

PRIDEiQ8ZIG8.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi214092.YPO0533.

Structurei

3D structure databases

ProteinModelPortaliQ8ZIG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZIG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DIMEVGFPVS
60 70 80 90 100
SPGDFESVRT IAQQVKNSRV CALARCVDKD IDVAAEALRI AEAFRIHVFL
110 120 130 140 150
ATSTLHIESK LKRSFDDVLA MAVHSVKRAR NYTDDVEFSC EDAGRTPIDN
160 170 180 190 200
LCRVVEAAIT AGATTINIPD TVGYTTPYQF GGIITDLYER VPNIDKAIIS
210 220 230 240 250
VHCHDDLGMS VANSITAVQA GARQVEGTIN GLGERAGNCS LEEVIMAIKV
260 270 280 290 300
RHEMLGVHTN INHQEIYRTS QLVSKICNMP IPGNKAIVGS NAFAHSSGIH
310 320 330 340 350
QDGVLKNREN YEIMTPESIG LKEVQLNLTS RSGRAAVKHR MEEMGYQDKD
360 370 380 390 400
YNLDSLYDAF LKLADKKGQV FDYDLEALAF INKQQEEPEY YRLDYFSVQS
410 420 430 440 450
GSSVMATASV KLVCGEEIKS EAATGNGPVD AVYQAINRIT DYPIELVKYQ
460 470 480 490 500
LSAKGQGKDA LGQVDIVVDH KGRRFHGVGL ATDIVESSAK ALVHVLNNIW
510 520
RAHQVEKEKQ RLQQNNQEMV
Length:520
Mass (Da):57,375
Last modified:March 1, 2002 - v1
Checksum:i7FC05F2FEEC0A472
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761T → P in AAM87193 (PubMed:12142430).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19213.1.
AE009952 Genomic DNA. Translation: AAM87193.1.
AE017042 Genomic DNA. Translation: AAS63797.1.
PIRiAC0066.
RefSeqiNP_670942.1. NC_004088.1.
NP_994920.1. NC_005810.1.
WP_002210453.1. NZ_KN150725.1.
YP_002345605.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM87193; AAM87193; y3645.
AAS63797; AAS63797; YP_3649.
GeneIDi1173378.
KEGGiype:YPO0533.
ypk:y3645.
ypm:YP_3649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19213.1.
AE009952 Genomic DNA. Translation: AAM87193.1.
AE017042 Genomic DNA. Translation: AAS63797.1.
PIRiAC0066.
RefSeqiNP_670942.1. NC_004088.1.
NP_994920.1. NC_005810.1.
WP_002210453.1. NZ_KN150725.1.
YP_002345605.1. NC_003143.1.

3D structure databases

ProteinModelPortaliQ8ZIG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi214092.YPO0533.

Proteomic databases

PRIDEiQ8ZIG8.

Protocols and materials databases

DNASUi1148592.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM87193; AAM87193; y3645.
AAS63797; AAS63797; YP_3649.
GeneIDi1173378.
KEGGiype:YPO0533.
ypk:y3645.
ypm:YP_3649.

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.
BioCyciYPES214092:GKDD-528-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiLEU1_YERPE
AccessioniPrimary (citable) accession number: Q8ZIG8
Secondary accession number(s): Q0WJD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.