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Q8ZIG8

- LEU1_YERPE

UniProt

Q8ZIG8 - LEU1_YERPE

Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Yersinia pestis
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

    Catalytic activityi

    Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. 2-isopropylmalate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Enzyme and pathway databases

    BioCyciYPES214092:GKDD-528-MONOMER.
    UniPathwayiUPA00048; UER00070.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
    Alternative name(s):
    Alpha-IPM synthaseUniRule annotation
    Alpha-isopropylmalate synthaseUniRule annotation
    Gene namesi
    Name:leuAUniRule annotation
    Ordered Locus Names:YPO0533, y3645, YP_3649
    OrganismiYersinia pestis
    Taxonomic identifieri632 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5205202-isopropylmalate synthasePRO_0000140404Add
    BLAST

    Proteomic databases

    PRIDEiQ8ZIG8.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi214092.YPO0533.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8ZIG8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0119.
    HOGENOMiHOG000046859.
    KOiK01649.
    OMAiEGQNALG.
    OrthoDBiEOG6CGCF3.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01025. LeuA_type1.
    InterProiIPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005671. LeuA_bact_synth.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view]
    SMARTiSM00917. LeuA_dimer. 1 hit.
    [Graphical view]
    SUPFAMiSSF110921. SSF110921. 1 hit.
    TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8ZIG8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DIMEVGFPVS    50
    SPGDFESVRT IAQQVKNSRV CALARCVDKD IDVAAEALRI AEAFRIHVFL 100
    ATSTLHIESK LKRSFDDVLA MAVHSVKRAR NYTDDVEFSC EDAGRTPIDN 150
    LCRVVEAAIT AGATTINIPD TVGYTTPYQF GGIITDLYER VPNIDKAIIS 200
    VHCHDDLGMS VANSITAVQA GARQVEGTIN GLGERAGNCS LEEVIMAIKV 250
    RHEMLGVHTN INHQEIYRTS QLVSKICNMP IPGNKAIVGS NAFAHSSGIH 300
    QDGVLKNREN YEIMTPESIG LKEVQLNLTS RSGRAAVKHR MEEMGYQDKD 350
    YNLDSLYDAF LKLADKKGQV FDYDLEALAF INKQQEEPEY YRLDYFSVQS 400
    GSSVMATASV KLVCGEEIKS EAATGNGPVD AVYQAINRIT DYPIELVKYQ 450
    LSAKGQGKDA LGQVDIVVDH KGRRFHGVGL ATDIVESSAK ALVHVLNNIW 500
    RAHQVEKEKQ RLQQNNQEMV 520
    Length:520
    Mass (Da):57,375
    Last modified:March 1, 2002 - v1
    Checksum:i7FC05F2FEEC0A472
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti176 – 1761T → P in AAM87193. (PubMed:12142430)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590842 Genomic DNA. Translation: CAL19213.1.
    AE009952 Genomic DNA. Translation: AAM87193.1.
    AE017042 Genomic DNA. Translation: AAS63797.1.
    PIRiAC0066.
    RefSeqiNP_670942.1. NC_004088.1.
    NP_994920.1. NC_005810.1.
    YP_002345605.1. NC_003143.1.

    Genome annotation databases

    EnsemblBacteriaiAAM87193; AAM87193; y3645.
    AAS63797; AAS63797; YP_3649.
    GeneIDi1148592.
    1173378.
    2766097.
    KEGGiype:YPO0533.
    ypk:y3645.
    ypm:YP_3649.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590842 Genomic DNA. Translation: CAL19213.1 .
    AE009952 Genomic DNA. Translation: AAM87193.1 .
    AE017042 Genomic DNA. Translation: AAS63797.1 .
    PIRi AC0066.
    RefSeqi NP_670942.1. NC_004088.1.
    NP_994920.1. NC_005810.1.
    YP_002345605.1. NC_003143.1.

    3D structure databases

    ProteinModelPortali Q8ZIG8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 214092.YPO0533.

    Proteomic databases

    PRIDEi Q8ZIG8.

    Protocols and materials databases

    DNASUi 1148592.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM87193 ; AAM87193 ; y3645 .
    AAS63797 ; AAS63797 ; YP_3649 .
    GeneIDi 1148592.
    1173378.
    2766097.
    KEGGi ype:YPO0533.
    ypk:y3645.
    ypm:YP_3649.

    Phylogenomic databases

    eggNOGi COG0119.
    HOGENOMi HOG000046859.
    KOi K01649.
    OMAi EGQNALG.
    OrthoDBi EOG6CGCF3.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00070 .
    BioCyci YPES214092:GKDD-528-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01025. LeuA_type1.
    InterProi IPR013709. 2-isopropylmalate_synth_dimer.
    IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR005671. LeuA_bact_synth.
    IPR000891. PYR_CT.
    [Graphical view ]
    Pfami PF00682. HMGL-like. 1 hit.
    PF08502. LeuA_dimer. 1 hit.
    [Graphical view ]
    SMARTi SM00917. LeuA_dimer. 1 hit.
    [Graphical view ]
    SUPFAMi SSF110921. SSF110921. 1 hit.
    TIGRFAMsi TIGR00973. leuA_bact. 1 hit.
    PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CO-92 / Biovar Orientalis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KIM10+ / Biovar Mediaevalis.
    3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
      Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
      , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
      DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 91001 / Biovar Mediaevalis.

    Entry informationi

    Entry nameiLEU1_YERPE
    AccessioniPrimary (citable) accession number: Q8ZIG8
    Secondary accession number(s): Q0WJD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 26, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3