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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA), 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB), 3-isopropylmalate dehydrogenase (leuB)
  4. Branched-chain-amino-acid aminotransferase (ilvE), Branched-chain-amino-acid aminotransferase (ilvE), Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:YPO0533, y3645, YP_3649
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404041 – 5202-isopropylmalate synthaseAdd BLAST520

Proteomic databases

PaxDbiQ8ZIG8

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y3645

Structurei

3D structure databases

ProteinModelPortaliQ8ZIG8
SMRiQ8ZIG8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 267Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST263

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CYQ Bacteria
COG0119 LUCA
HOGENOMiHOG000046859
KOiK01649
OMAiKFHGVGL

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01025 LeuA_type1, 1 hit
InterProiView protein in InterPro
IPR013709 2-isopropylmalate_synth_dimer
IPR002034 AIPM/Hcit_synth_CS
IPR013785 Aldolase_TIM
IPR036230 LeuA_allosteric_dom_sf
IPR005671 LeuA_bact_synth
IPR000891 PYR_CT
PfamiView protein in Pfam
PF00682 HMGL-like, 1 hit
PF08502 LeuA_dimer, 1 hit
SMARTiView protein in SMART
SM00917 LeuA_dimer, 1 hit
SUPFAMiSSF110921 SSF110921, 1 hit
TIGRFAMsiTIGR00973 leuA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00815 AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816 AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZIG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DIMEVGFPVS
60 70 80 90 100
SPGDFESVRT IAQQVKNSRV CALARCVDKD IDVAAEALRI AEAFRIHVFL
110 120 130 140 150
ATSTLHIESK LKRSFDDVLA MAVHSVKRAR NYTDDVEFSC EDAGRTPIDN
160 170 180 190 200
LCRVVEAAIT AGATTINIPD TVGYTTPYQF GGIITDLYER VPNIDKAIIS
210 220 230 240 250
VHCHDDLGMS VANSITAVQA GARQVEGTIN GLGERAGNCS LEEVIMAIKV
260 270 280 290 300
RHEMLGVHTN INHQEIYRTS QLVSKICNMP IPGNKAIVGS NAFAHSSGIH
310 320 330 340 350
QDGVLKNREN YEIMTPESIG LKEVQLNLTS RSGRAAVKHR MEEMGYQDKD
360 370 380 390 400
YNLDSLYDAF LKLADKKGQV FDYDLEALAF INKQQEEPEY YRLDYFSVQS
410 420 430 440 450
GSSVMATASV KLVCGEEIKS EAATGNGPVD AVYQAINRIT DYPIELVKYQ
460 470 480 490 500
LSAKGQGKDA LGQVDIVVDH KGRRFHGVGL ATDIVESSAK ALVHVLNNIW
510 520
RAHQVEKEKQ RLQQNNQEMV
Length:520
Mass (Da):57,375
Last modified:March 1, 2002 - v1
Checksum:i7FC05F2FEEC0A472
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176T → P in AAM87193 (PubMed:12142430).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19213.1
AE009952 Genomic DNA Translation: AAM87193.1
AE017042 Genomic DNA Translation: AAS63797.1
PIRiAC0066
RefSeqiWP_002210453.1, NZ_LT605019.1
YP_002345605.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM87193; AAM87193; y3645
AAS63797; AAS63797; YP_3649
GeneIDi1173378
KEGGiype:YPO0533
ypj:CH55_2272
ypk:y3645
ypl:CH46_381
ypm:YP_3649
ypv:BZ15_3037
ypw:CH59_1328
PATRICifig|214092.21.peg.786

Similar proteinsi

Entry informationi

Entry nameiLEU1_YERPE
AccessioniPrimary (citable) accession number: Q8ZIG8
Secondary accession number(s): Q0WJD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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