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Protein

UDP-N-acetylmuramate--L-alanine ligase

Gene

murC

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi126 – 1327ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-550-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramate--L-alanine ligaseUniRule annotation (EC:6.3.2.8UniRule annotation)
Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetaseUniRule annotation
Gene namesi
Name:murCUniRule annotation
Ordered Locus Names:YPO0556, y3625, YP_3628
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491UDP-N-acetylmuramate--L-alanine ligasePRO_0000182190Add
BLAST

Proteomic databases

PRIDEiQ8ZIE8.

Interactioni

Protein-protein interaction databases

IntActiQ8ZIE8. 1 interaction.
STRINGi187410.y3625.

Structurei

Secondary structure

1
491
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 183Combined sources
Beta strandi21 – 255Combined sources
Turni26 – 283Combined sources
Helixi32 – 4110Combined sources
Beta strandi45 – 495Combined sources
Helixi55 – 628Combined sources
Beta strandi66 – 705Combined sources
Helixi73 – 764Combined sources
Beta strandi80 – 845Combined sources
Helixi93 – 1008Combined sources
Beta strandi105 – 1073Combined sources
Helixi108 – 1169Combined sources
Beta strandi119 – 1257Combined sources
Beta strandi127 – 1293Combined sources
Helixi130 – 14314Combined sources
Beta strandi149 – 1568Combined sources
Turni157 – 1604Combined sources
Beta strandi161 – 1644Combined sources
Beta strandi167 – 1748Combined sources
Helixi178 – 1847Combined sources
Beta strandi188 – 1925Combined sources
Helixi206 – 21813Combined sources
Beta strandi226 – 2305Combined sources
Helixi234 – 2396Combined sources
Helixi240 – 2423Combined sources
Beta strandi247 – 2537Combined sources
Beta strandi257 – 26610Combined sources
Beta strandi269 – 2757Combined sources
Beta strandi282 – 2887Combined sources
Helixi291 – 30717Combined sources
Helixi311 – 32010Combined sources
Beta strandi327 – 33610Combined sources
Helixi337 – 3404Combined sources
Beta strandi343 – 3519Combined sources
Helixi356 – 36914Combined sources
Beta strandi373 – 3797Combined sources
Helixi384 – 3896Combined sources
Helixi391 – 3988Combined sources
Beta strandi401 – 4077Combined sources
Helixi422 – 4309Combined sources
Beta strandi438 – 4403Combined sources
Turni443 – 4453Combined sources
Helixi446 – 4538Combined sources
Beta strandi458 – 4636Combined sources
Helixi468 – 47710Combined sources
Turni478 – 4803Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HV4X-ray2.25A/B1-491[»]
ProteinModelPortaliQ8ZIE8.
SMRiQ8ZIE8. Positions 1-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DFU. Bacteria.
COG0773. LUCA.
HOGENOMiHOG000256031.
KOiK01924.
OMAiFHFIGIG.
OrthoDBiEOG64BQ73.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00046. MurC.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase_murC.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01082. murC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZIE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTQQLAKLR TIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD
60 70 80 90 100
LAPNSVTQHL TALGAQIYFH HRPENVLDAS VVVVSTAISA DNPEIVAARE
110 120 130 140 150
ARIPVIRRAE MLAELMRYRH GIAVAGTHGK TTTTAMLSSI YAEAGLDPTF
160 170 180 190 200
VNGGLVKAAG THARLGSSRY LIAEADESDA SFLHLQPMVA IVTNIEADHM
210 220 230 240 250
DTYQGDFENL KQTFINFLHN LPFYGRAVMC IDDPVVRELL PRVGRHITTY
260 270 280 290 300
GFSDDADVQI ASYRQEGPQG HFTLRRQDKP LIEVTLNAPG RHNALNAAAA
310 320 330 340 350
VAVATEEGIE DEDILRALVG FQGTGRRFDF LGNFPLAPVN GKEGSAMLVD
360 370 380 390 400
DYGHHPTEVD ATIKAARAGW PDKRIVMLFQ PHRYTRTRDL YDDFANVLSQ
410 420 430 440 450
VDVLLMLDVY AAGEPPIPGA DSRALCRTIR NRGKLDPILV PDSESAPEML
460 470 480 490
AQILNGEDLI LVQGAGNIGK IARKLAEHKL QPQLKDEEHH G
Length:491
Mass (Da):53,654
Last modified:March 1, 2002 - v1
Checksum:i852C270C93370759
GO

Sequence cautioni

The sequence AAM87173.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAS63776.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19235.1.
AE009952 Genomic DNA. Translation: AAM87173.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS63776.1. Different initiation.
PIRiAI0068.
RefSeqiWP_002216457.1. NZ_LQAY01000071.1.
YP_002345627.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM87173; AAM87173; y3625.
AAS63776; AAS63776; YP_3628.
GeneIDi1173400.
KEGGiype:YPO0556.
ypj:CH55_2293.
ypk:y3625.
ypl:CH46_361.
ypm:YP_3628.
ypv:BZ15_3018.
ypw:CH59_1308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19235.1.
AE009952 Genomic DNA. Translation: AAM87173.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS63776.1. Different initiation.
PIRiAI0068.
RefSeqiWP_002216457.1. NZ_LQAY01000071.1.
YP_002345627.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HV4X-ray2.25A/B1-491[»]
ProteinModelPortaliQ8ZIE8.
SMRiQ8ZIE8. Positions 1-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8ZIE8. 1 interaction.
STRINGi187410.y3625.

Proteomic databases

PRIDEiQ8ZIE8.

Protocols and materials databases

DNASUi1148572.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM87173; AAM87173; y3625.
AAS63776; AAS63776; YP_3628.
GeneIDi1173400.
KEGGiype:YPO0556.
ypj:CH55_2293.
ypk:y3625.
ypl:CH46_361.
ypm:YP_3628.
ypv:BZ15_3018.
ypw:CH59_1308.

Phylogenomic databases

eggNOGiENOG4105DFU. Bacteria.
COG0773. LUCA.
HOGENOMiHOG000256031.
KOiK01924.
OMAiFHFIGIG.
OrthoDBiEOG64BQ73.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciYPES214092:GKDD-550-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00046. MurC.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase_murC.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01082. murC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiMURC_YERPE
AccessioniPrimary (citable) accession number: Q8ZIE8
Secondary accession number(s): Q0WJB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: March 1, 2002
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.