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Protein

UDP-N-acetylmuramate--L-alanine ligase

Gene

murC

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 132ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramate--L-alanine ligaseUniRule annotation (EC:6.3.2.8UniRule annotation)
Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetaseUniRule annotation
Gene namesi
Name:murCUniRule annotation
Ordered Locus Names:YPO0556, y3625, YP_3628
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001821901 – 491UDP-N-acetylmuramate--L-alanine ligaseAdd BLAST491

Proteomic databases

PRIDEiQ8ZIE8.

Interactioni

Protein-protein interaction databases

IntActiQ8ZIE8. 1 interactor.
STRINGi187410.y3625.

Structurei

Secondary structure

1491
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 18Combined sources3
Beta strandi21 – 25Combined sources5
Turni26 – 28Combined sources3
Helixi32 – 41Combined sources10
Beta strandi45 – 49Combined sources5
Helixi55 – 62Combined sources8
Beta strandi66 – 70Combined sources5
Helixi73 – 76Combined sources4
Beta strandi80 – 84Combined sources5
Helixi93 – 100Combined sources8
Beta strandi105 – 107Combined sources3
Helixi108 – 116Combined sources9
Beta strandi119 – 125Combined sources7
Beta strandi127 – 129Combined sources3
Helixi130 – 143Combined sources14
Beta strandi149 – 156Combined sources8
Turni157 – 160Combined sources4
Beta strandi161 – 164Combined sources4
Beta strandi167 – 174Combined sources8
Helixi178 – 184Combined sources7
Beta strandi188 – 192Combined sources5
Helixi206 – 218Combined sources13
Beta strandi226 – 230Combined sources5
Helixi234 – 239Combined sources6
Helixi240 – 242Combined sources3
Beta strandi247 – 253Combined sources7
Beta strandi257 – 266Combined sources10
Beta strandi269 – 275Combined sources7
Beta strandi282 – 288Combined sources7
Helixi291 – 307Combined sources17
Helixi311 – 320Combined sources10
Beta strandi327 – 336Combined sources10
Helixi337 – 340Combined sources4
Beta strandi343 – 351Combined sources9
Helixi356 – 369Combined sources14
Beta strandi373 – 379Combined sources7
Helixi384 – 389Combined sources6
Helixi391 – 398Combined sources8
Beta strandi401 – 407Combined sources7
Helixi422 – 430Combined sources9
Beta strandi438 – 440Combined sources3
Turni443 – 445Combined sources3
Helixi446 – 453Combined sources8
Beta strandi458 – 463Combined sources6
Helixi468 – 477Combined sources10
Turni478 – 480Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HV4X-ray2.25A/B1-491[»]
ProteinModelPortaliQ8ZIE8.
SMRiQ8ZIE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DFU. Bacteria.
COG0773. LUCA.
HOGENOMiHOG000256031.
KOiK01924.
OMAiFHFIGIG.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00046. MurC. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase_murC.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01082. murC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZIE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTQQLAKLR TIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD
60 70 80 90 100
LAPNSVTQHL TALGAQIYFH HRPENVLDAS VVVVSTAISA DNPEIVAARE
110 120 130 140 150
ARIPVIRRAE MLAELMRYRH GIAVAGTHGK TTTTAMLSSI YAEAGLDPTF
160 170 180 190 200
VNGGLVKAAG THARLGSSRY LIAEADESDA SFLHLQPMVA IVTNIEADHM
210 220 230 240 250
DTYQGDFENL KQTFINFLHN LPFYGRAVMC IDDPVVRELL PRVGRHITTY
260 270 280 290 300
GFSDDADVQI ASYRQEGPQG HFTLRRQDKP LIEVTLNAPG RHNALNAAAA
310 320 330 340 350
VAVATEEGIE DEDILRALVG FQGTGRRFDF LGNFPLAPVN GKEGSAMLVD
360 370 380 390 400
DYGHHPTEVD ATIKAARAGW PDKRIVMLFQ PHRYTRTRDL YDDFANVLSQ
410 420 430 440 450
VDVLLMLDVY AAGEPPIPGA DSRALCRTIR NRGKLDPILV PDSESAPEML
460 470 480 490
AQILNGEDLI LVQGAGNIGK IARKLAEHKL QPQLKDEEHH G
Length:491
Mass (Da):53,654
Last modified:March 1, 2002 - v1
Checksum:i852C270C93370759
GO

Sequence cautioni

The sequence AAM87173 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS63776 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19235.1.
AE009952 Genomic DNA. Translation: AAM87173.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS63776.1. Different initiation.
PIRiAI0068.
RefSeqiWP_002216457.1. NZ_LQBA01000066.1.
YP_002345627.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM87173; AAM87173; y3625.
AAS63776; AAS63776; YP_3628.
GeneIDi1173400.
KEGGiype:YPO0556.
ypk:y3625.
ypm:YP_3628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19235.1.
AE009952 Genomic DNA. Translation: AAM87173.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS63776.1. Different initiation.
PIRiAI0068.
RefSeqiWP_002216457.1. NZ_LQBA01000066.1.
YP_002345627.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HV4X-ray2.25A/B1-491[»]
ProteinModelPortaliQ8ZIE8.
SMRiQ8ZIE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8ZIE8. 1 interactor.
STRINGi187410.y3625.

Proteomic databases

PRIDEiQ8ZIE8.

Protocols and materials databases

DNASUi1148572.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM87173; AAM87173; y3625.
AAS63776; AAS63776; YP_3628.
GeneIDi1173400.
KEGGiype:YPO0556.
ypk:y3625.
ypm:YP_3628.

Phylogenomic databases

eggNOGiENOG4105DFU. Bacteria.
COG0773. LUCA.
HOGENOMiHOG000256031.
KOiK01924.
OMAiFHFIGIG.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00046. MurC. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase_murC.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01082. murC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURC_YERPE
AccessioniPrimary (citable) accession number: Q8ZIE8
Secondary accession number(s): Q0WJB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.