Reviewed, UniProtKB/Swiss-Prot Q8ZI40 (DKGA_YERPE)

Names and origin

Protein names

Recommended name:
    2,5-diketo-D-gluconic acid reductase A
      Short name=2,5-DKG reductase A
      Short name=2,5-DKGR A
      Short name=25DKGR-A
    EC=1.1.1.274
Alternative name(s):
    AKR5C

Gene names

Name:	dkgA
Synonyms:	ara14
Ordered Locus Names:	YPO0676, y3501, YP_2991

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) By similarity.
Catalytic activity	2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Ascorbate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-ascorbic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,5-didehydrogluconate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	2,5-diketo-D-gluconic acid reductase A		PRO_0000124603
Regions
	Nucleotide binding	187 – 241	55	NADP By similarity
Sites
	Active site	51	1	Proton donor By similarity
	Binding site	107	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZI40-1 [UniParc]. Last modified March 27, 2002. Version 1. Checksum: ED9C2945F0EBD140	FASTA	277	31,520
	10 20 30 40 50 60 MTMQPLIKLY DGRLMPQLGL GVWQASIQET ELAVSKALEV GYRSIDTAAI YKNEEGVGKA 70 80 90 100 110 120 LKAAAVARDE LFITTKLWND DQHNPQQALE TSLQKLQLDY VDLYLIHWPD PKQDHYVSAW 130 140 150 160 170 180 RELVTLKEQG LIRSIGVCNF HIPHLQRLID ETGIAPTVNQ IELHPLLQQR QLHAWNATHH 190 200 210 220 230 240 IATESWSPLA QGGKGVFDQE IIRKLAQQYN KTPAQIVIRW HLDSGLIVIP KSVTPARIRE 250 260 270 NFEVFDFKLQ KEELLAITKL DCGKRLGPDP EVFGSDR

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL590842 Genomic DNA. Translation: CAL19352.1. AE009952 Genomic DNA. Translation: AAM87049.1. AE017042 Genomic DNA. Translation: AAS63169.1.
PIR	AF0083.
RefSeq	NP_670798.1. NP_994292.1. YP_002345742.1.
3D structure databases
HSSP	HSSP built from PDB template 1HW6 based on UniProtKB P06632.
SMR	Q8ZI40. Positions 6-274.
ModBase	Search...
Genome annotation databases
GeneID	1148448. 1173519. 2766792.
GenomeReviews	Gene locus y3501 in contig AE009952_GR. Gene locus YP_2991 in contig AE017042_GR. Gene locus YPO0676 in contig AL590842_GR.
KEGG	ype:YPO0676. ypk:y3501. ypm:YP_2991.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZI40.
OMA	Q8ZI40. GIAVEAY.
Enzyme and pathway databases
BioCyc	YPES187410:Y3501-MON. YPES214092:YPO0676-MON. YPES229193:YP2991-MON.
BRENDA	1.1.1.274. 142588.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DKGA_YERPE

Accession

Primary (citable) accession number: Q8ZI40
Secondary accession number(s): Q0WIZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)