Q8ZHF1 (GLSA1_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase 1
EC=3.5.1.2

Gene names

Name:	glsA1
Ordered Locus Names:	YPO0949, y3336, YP_3492

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Glutaminase 1 HAMAP-Rule MF_00313		PRO_0000110633
Sites
	Binding site	66	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	262	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZHF1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D80AD7C77F23FEC3	FASTA	308	33,700
	10 20 30 40 50 60 MVTTLDNALL NDILQQVRPL IGQGKVADYI PALAEVPANK LGIAVCTLDG QIFQAGDADE 70 80 90 100 110 120 RFSIQSISKV LSLTLALSRY SEQDIWQRVG KEPSGQPFNS LVQLELEKGK PRNPFINLGA 130 140 150 160 170 180 LVVCDMLQSR LSAPKQRLLE VVRQLVKDDS ISYDPRVARS EFEHSDRNAA IAYLMKSFGN 190 200 210 220 230 240 FDNDVLTVLQ TYFHYCAMRM SCVELARCFV YLANQGRSIS GSESLITPMQ ARQINALMIT 250 260 270 280 290 300 SGMYDGAGEF AFRVGMPGKS GVGGGIIAIV PDEFCIAVWS PELDHAGNSL AGTAALERLA QQMGRSIF

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL19615.1. AE009952 Genomic DNA. Translation: AAM86886.1. AE017042 Genomic DNA. Translation: AAS63647.1.
PIR	AE0116.
RefSeq	NP_670635.1. NC_004088.1. NP_994770.1. NC_005810.1. YP_002345995.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZHF1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8ZHF1. 5 interactions.
STRING	214092.YPO0949.
Protocols and materials databases
DNASU	1148283.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM86886; AAM86886; y3336. AAS63647; AAS63647; YP_3492.
GeneID	1148283. 1173785. 2765271.
KEGG	ype:YPO0949. ypk:y3336. ypm:YP_3492.
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	EPSGNPF.
ProtClustDB	PRK00971.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA1_YERPE

Accession

Primary (citable) accession number: Q8ZHF1
Secondary accession number(s): Q0WI93

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program