Q8ZHE9 (TRMB_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	YPO0951, y3338, YP_3490

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Subunit structure	Monomer By similarity. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171428
Regions
	Region	150 – 155	6	Interaction with RNA Potential
	Region	217 – 220	4	Substrate binding Potential
Sites
	Binding site	69	1	S-adenosyl-L-methionine By similarity
	Binding site	94	1	S-adenosyl-L-methionine By similarity
	Binding site	121	1	S-adenosyl-L-methionine By similarity
	Binding site	144	1	S-adenosyl-L-methionine By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	180	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8ZHE9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 90C9B9CF979AB0BB	FASTA	239	27,019
	10 20 30 40 50 60 MINDVISPEF DENGRALRRI RSFVRRQGRL TKGQQLALDS YWPVMGVEYQ AAPVDLNTLF 70 80 90 100 110 120 GREAPIVLEI GFGMGTSLVT MATNNPQQNF LGIEVHSPGV GACLSSAHDA GLSNLRIMCH 130 140 150 160 170 180 DAVEVLENMI PEASLDMVQL FFPDPWHKAR HNKRRIVQTP FVELVKSKLK VGGVFHMATD 190 200 210 220 230 WQPYAEHMLE VMSGVSGYLN LSEQNDYVPR PDSRPLTKFE LRGQRLGHGV WDLMFERKE

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL19617.1. AE009952 Genomic DNA. Translation: AAM86888.1. AE017042 Genomic DNA. Translation: AAS63645.1.
PIR	AG0116.
RefSeq	NP_670637.1. NC_004088.1. NP_994768.1. NC_005810.1. YP_002345997.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZHE9.
ModBase	Search...
Protein-protein interaction databases
STRING	214092.YPO0951.
Protocols and materials databases
DNASU	1148285.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM86888; AAM86888; y3338. AAS63645; AAS63645; YP_3490.
GeneID	1148285. 1173787. 2763566.
KEGG	ype:YPO0951. ypk:y3338. ypm:YP_3490.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000073968.
KO	K03439.
OMA	CEVHEPG.
ProtClustDB	PRK00121.
Enzyme and pathway databases
BioCyc	YPES214092:GKDD-935-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_YERPE

Accession

Primary (citable) accession number: Q8ZHE9
Secondary accession number(s): Q0WI91

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program