Q8ZH36 (DKGB_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,5-diketo-D-gluconic acid reductase B
Short name=2,5-DKG reductase B
Short name=2,5-DKGR B
Short name=25DKGR-B
EC=1.1.1.274

Alternative name(s):
AKR5D

Gene names

Name:	dkgB
Synonyms:	ara13
Ordered Locus Names:	YPO1075, y3101, YP_2774

Organism

Yersinia pestis

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) By similarity.
Catalytic activity	2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Ascorbate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-ascorbic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,5-didehydrogluconate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	2,5-diketo-D-gluconic acid reductase B		PRO_0000124608
Regions
	Nucleotide binding	179 – 231	53	NADP By similarity
Sites
	Active site	39	1	Proton donor By similarity
	Binding site	97	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZH36 [UniParc]. Last modified March 27, 2002. Version 1. Checksum: 1E5AC57827C31AF5	FASTA	267	29,035
	10 20 30 40 50 60 MSIPVFGLGT FRLQDQIVID SVSQALTLGY RAIDTAQIYE NEAPVGQAIQ ESGINRDELF 70 80 90 100 110 120 ITTKIWIANL SKDKLIPSLR ESIQKLKTDY VDLTLIHWPS PNDEVSVAEF MSELLKAKGL 130 140 150 160 170 180 GLTRQIGISN FTIDLMKQAI AAVGAEEIAT NQIELSPLLQ NRNVVDFAKQ NGIAVTSYMT 190 200 210 220 230 240 LAYGKALAEP VIKTIAEQHG ATPAQVILSW AMQLGYGVIP SSTKAANLAS NLLAQKLCLN 250 260 AADMALIATL DRNERLVSPD GLAPKWD

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL19741.1. AE009952 Genomic DNA. Translation: AAM86651.1. AE017042 Genomic DNA. Translation: AAS62958.1.
PIR	AC0132.
RefSeq	NP_670400.1. NC_004088.1. NP_994081.1. NC_005810.1. YP_002346119.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZH36.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1148048. 1173921. 2764883.
GenomeReviews	Gene locus y3101 in contig AE009952_GR. Gene locus YP_2774 in contig AE017042_GR. Gene locus YPO1075 in contig AL590842_GR.
KEGG	ype:YPO1075. ypk:y3101. ypm:YP_2774.
Phylogenomic databases
HOGENOM	HBG605727.
OMA	LAWAMQL.
ProtClustDB	PRK11172.
Enzyme and pathway databases
BioCyc	YPES187410:Y3101-MONOMER. YPES214092:YPO1075-MONOMER.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_subgr. IPR023210. NADP_OxRdtase_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
KO	K06222.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PIRSF	PIRSF000097. AKR. 1 hit.
PRINTS	PR00069. ALDKETRDTASE.
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DKGB_YERPE

Accession

Primary (citable) accession number: Q8ZH36
Secondary accession number(s): Q0WHW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program