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Q8ZH09

- NADK_YERPE

UniProt

Q8ZH09 - NADK_YERPE

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Protein
NAD kinase
Gene
nadK, YPO1106, y3074, YP_1050
Organism
Yersinia pestis
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

Divalent metal ions By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741Proton acceptor By similarity
Binding sitei159 – 1591NAD By similarity
Binding sitei176 – 1761NAD By similarity
Sitei176 – 1761Responsible for conferring strict specificity to NAD By similarity
Binding sitei178 – 1781NAD By similarity
Binding sitei186 – 1861NAD; via carbonyl oxygen By similarity
Binding sitei248 – 2481NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 752NAD By similarity
Nucleotide bindingi148 – 1492NAD By similarity
Nucleotide bindingi189 – 1946NAD By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-1101-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase (EC:2.7.1.23)
Alternative name(s):
ATP-dependent NAD kinase
Gene namesi
Name:nadK
Ordered Locus Names:YPO1106, y3074, YP_1050
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293NAD kinaseUniRule annotation
PRO_0000120695Add
BLAST

Proteomic databases

PRIDEiQ8ZH09.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ8ZH09. 1 interaction.
STRINGi214092.YPO1106.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126
Helixi21 – 3313
Beta strandi37 – 415
Helixi42 – 487
Beta strandi54 – 563
Helixi58 – 647
Beta strandi66 – 727
Helixi74 – 8411
Turni85 – 884
Beta strandi90 – 945
Beta strandi96 – 983
Helixi107 – 1093
Helixi110 – 11910
Beta strandi122 – 13514
Beta strandi142 – 15615
Beta strandi161 – 1677
Beta strandi170 – 18314
Helixi186 – 1894
Helixi191 – 1944
Beta strandi205 – 2139
Beta strandi222 – 2254
Beta strandi230 – 2356
Beta strandi241 – 2455
Beta strandi251 – 2533
Beta strandi258 – 27316
Helixi278 – 2869

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HAOX-ray2.55A/B1-293[»]
ProteinModelPortaliQ8ZH09.
SMRiQ8ZH09. Positions 6-293.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiIRRASCA.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZH09-1 [UniParc]FASTAAdd to Basket

« Hide

MNNRRFDCIG IVGHPRHPAA LATHEILYHW LKARGYAVMV EQQIAHDLNL    50
TDAITGSLAD IGQKADLAVV VGGDGNMLGA ARVLARYDIK VIGVNRGNLG 100
FLTDLDPDNA LQQLSDVLEG EYLSEQRFLL ETHVRRTNQQ SRISTAINEV 150
VLHPGKVAHM IEFEVYIDDR FAFSQRSDGL IIATPTGSTA YSLSAGGPIL 200
TPTLDAIVLV PMFPHTLTAR PLVISSSSTI RLKFSHITSD LEISCDSQIA 250
LPIQEGEEVL IRRSDFHLNL IHPKDYSYFN TLSTKLGWSK KLF 293
Length:293
Mass (Da):32,398
Last modified:March 1, 2002 - v1
Checksum:iC0231F341E8F4A1E
GO

Sequence cautioni

The sequence AAS61300.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL19772.1.
AE009952 Genomic DNA. Translation: AAM86624.1.
AE017042 Genomic DNA. Translation: AAS61300.1. Different initiation.
PIRiAB0136.
RefSeqiNP_670373.1. NC_004088.1.
YP_002346149.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM86624; AAM86624; y3074.
AAS61300; AAS61300; YP_1050.
GeneIDi1148021.
1173954.
KEGGiype:YPO1106.
ypk:y3074.
PATRICi18591493. VBIYerPes7843_1474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL19772.1 .
AE009952 Genomic DNA. Translation: AAM86624.1 .
AE017042 Genomic DNA. Translation: AAS61300.1 . Different initiation.
PIRi AB0136.
RefSeqi NP_670373.1. NC_004088.1.
YP_002346149.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HAO X-ray 2.55 A/B 1-293 [» ]
ProteinModelPortali Q8ZH09.
SMRi Q8ZH09. Positions 6-293.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8ZH09. 1 interaction.
STRINGi 214092.YPO1106.

Proteomic databases

PRIDEi Q8ZH09.

Protocols and materials databases

DNASUi 1148021.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM86624 ; AAM86624 ; y3074 .
AAS61300 ; AAS61300 ; YP_1050 .
GeneIDi 1148021.
1173954.
KEGGi ype:YPO1106.
ypk:y3074.
PATRICi 18591493. VBIYerPes7843_1474.

Phylogenomic databases

eggNOGi COG0061.
HOGENOMi HOG000227221.
KOi K00858.
OMAi IRRASCA.
OrthoDBi EOG6PZXDR.

Enzyme and pathway databases

BioCyci YPES214092:GKDD-1101-MONOMER.

Family and domain databases

Gene3Di 2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPi MF_00361. NAD_kinase.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view ]
PANTHERi PTHR20275. PTHR20275. 1 hit.
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.
  4. "Crystal structure of inorganic polyphosphate/ATP-NAD kinase from Yersinia pestis CO92."
    Center for structural genomics of infectious diseases (CSGID)
    Submitted (SEP-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiNADK_YERPE
AccessioniPrimary (citable) accession number: Q8ZH09
Secondary accession number(s): Q0WHT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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