Q8ZH09 (PPNK_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	YPO1106, y3074, YP_1050

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.
Sequence caution	The sequence AAS61300.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 293

293

Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361

PRO_0000120695

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

293

Helix Strand Turn

Details...

Beta strand

7 – 12

6

Helix

21 – 33

13

Beta strand

37 – 41

5

Helix

42 – 48

7

Beta strand

54 – 56

3

Helix

58 – 64

7

Beta strand

66 – 72

7

Helix

74 – 84

11

Turn

85 – 88

4

Beta strand

90 – 94

5

Beta strand

96 – 98

3

Helix

107 – 109

3

Helix

110 – 119

10

Beta strand

122 – 135

14

Beta strand

142 – 156

15

Beta strand

161 – 167

7

Beta strand

170 – 183

14

Helix

186 – 189

4

Helix

191 – 194

4

Beta strand

205 – 213

9

Beta strand

222 – 225

4

Beta strand

230 – 235

6

Beta strand

241 – 245

5

Beta strand

251 – 253

3

Beta strand

258 – 273

16

Helix

278 – 286

9

Sequences

	Sequence		Length	Mass (Da)
	Q8ZH09 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C0231F341E8F4A1E	FASTA	293	32,398
	10 20 30 40 50 60 MNNRRFDCIG IVGHPRHPAA LATHEILYHW LKARGYAVMV EQQIAHDLNL TDAITGSLAD 70 80 90 100 110 120 IGQKADLAVV VGGDGNMLGA ARVLARYDIK VIGVNRGNLG FLTDLDPDNA LQQLSDVLEG 130 140 150 160 170 180 EYLSEQRFLL ETHVRRTNQQ SRISTAINEV VLHPGKVAHM IEFEVYIDDR FAFSQRSDGL 190 200 210 220 230 240 IIATPTGSTA YSLSAGGPIL TPTLDAIVLV PMFPHTLTAR PLVISSSSTI RLKFSHITSD 250 260 270 280 290 LEISCDSQIA LPIQEGEEVL IRRSDFHLNL IHPKDYSYFN TLSTKLGWSK KLF

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL590842 Genomic DNA. Translation: CAL19772.1.
AE009952 Genomic DNA. Translation: AAM86624.1.
AE017042 Genomic DNA. Translation: AAS61300.1. Different initiation.

PIR

AB0136.

RefSeq

NP_670373.1. NC_004088.1.
YP_002346149.1. NC_003143.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HAO	X-ray	2.55	A/B	1-293	[»]

ProteinModelPortal

Q8ZH09.

SMR

Q8ZH09. Positions 6-293.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8ZH09. 1 interaction.

STRING

214092.YPO1106.

Proteomic databases

PRIDE

Q8ZH09.

Protocols and materials databases

DNASU

1148021.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAM86624; AAM86624; y3074.
AAS61300; AAS61300; YP_1050.

GeneID

1148021.
1173954.

KEGG

ype:YPO1106.
ypk:y3074.
ypm:YP_1050.

PATRIC

18591493. VBIYerPes7843_1474.

Phylogenomic databases

eggNOG

COG0061.

HOGENOM

HOG000227221.

KO

K00858.

OMA

HRITERM.

ProtClustDB

PRK03378.

Family and domain databases

Gene3D

2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.

HAMAP

MF_00361. NAD_kinase.

InterPro

IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK_prd.
[Graphical view]

PANTHER

PTHR20275. PTHR20275. 1 hit.

Pfam

PF01513. NAD_kinase. 1 hit.
[Graphical view]

SUPFAM

SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PPNK_YERPE

Accession

Primary (citable) accession number: Q8ZH09
Secondary accession number(s): Q0WHT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program