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Protein

NAD kinase

Gene

nadK

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74Proton acceptorUniRule annotation1
Binding sitei159NADUniRule annotation1
Binding sitei176NADUniRule annotation1
Sitei176Responsible for conferring strict specificity to NADBy similarity1
Binding sitei178NADUniRule annotation1
Binding sitei186NAD; via carbonyl oxygenUniRule annotation1
Binding sitei248NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 75NADUniRule annotation2
Nucleotide bindingi148 – 149NADUniRule annotation2
Nucleotide bindingi189 – 194NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:YPO1106, y3074, YP_1050
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001206951 – 293NAD kinaseAdd BLAST293

Proteomic databases

PaxDbiQ8ZH09
PRIDEiQ8ZH09

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ8ZH09, 1 interactor
STRINGi187410.y3074

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Helixi21 – 33Combined sources13
Beta strandi37 – 41Combined sources5
Helixi42 – 48Combined sources7
Beta strandi54 – 56Combined sources3
Helixi58 – 64Combined sources7
Beta strandi66 – 72Combined sources7
Helixi74 – 84Combined sources11
Turni85 – 88Combined sources4
Beta strandi90 – 94Combined sources5
Beta strandi96 – 98Combined sources3
Helixi107 – 109Combined sources3
Helixi110 – 119Combined sources10
Beta strandi122 – 135Combined sources14
Beta strandi142 – 156Combined sources15
Beta strandi161 – 167Combined sources7
Beta strandi170 – 183Combined sources14
Helixi186 – 189Combined sources4
Helixi191 – 194Combined sources4
Beta strandi205 – 213Combined sources9
Beta strandi222 – 225Combined sources4
Beta strandi230 – 235Combined sources6
Beta strandi241 – 245Combined sources5
Beta strandi251 – 253Combined sources3
Beta strandi258 – 273Combined sources16
Helixi278 – 286Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HAOX-ray2.55A/B1-293[»]
ProteinModelPortaliQ8ZH09
SMRiQ8ZH09
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F91 Bacteria
COG0061 LUCA
HOGENOMiHOG000227221
KOiK00858
OMAiVNLGHVG

Family and domain databases

Gene3Di2.60.200.30, 1 hit
3.40.50.10330, 2 hits
HAMAPiMF_00361 NAD_kinase, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR017437 ATP-NAD_kinase_PpnK-typ_C
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002504 NADK
PANTHERiPTHR20275 PTHR20275, 1 hit
PfamiView protein in Pfam
PF01513 NAD_kinase, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZH09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNRRFDCIG IVGHPRHPAA LATHEILYHW LKARGYAVMV EQQIAHDLNL
60 70 80 90 100
TDAITGSLAD IGQKADLAVV VGGDGNMLGA ARVLARYDIK VIGVNRGNLG
110 120 130 140 150
FLTDLDPDNA LQQLSDVLEG EYLSEQRFLL ETHVRRTNQQ SRISTAINEV
160 170 180 190 200
VLHPGKVAHM IEFEVYIDDR FAFSQRSDGL IIATPTGSTA YSLSAGGPIL
210 220 230 240 250
TPTLDAIVLV PMFPHTLTAR PLVISSSSTI RLKFSHITSD LEISCDSQIA
260 270 280 290
LPIQEGEEVL IRRSDFHLNL IHPKDYSYFN TLSTKLGWSK KLF
Length:293
Mass (Da):32,398
Last modified:March 1, 2002 - v1
Checksum:iC0231F341E8F4A1E
GO

Sequence cautioni

The sequence AAS61300 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19772.1
AE009952 Genomic DNA Translation: AAM86624.1
AE017042 Genomic DNA Translation: AAS61300.1 Different initiation.
PIRiAB0136
RefSeqiWP_002210719.1, NZ_PDBR01000020.1
YP_002346149.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM86624; AAM86624; y3074
AAS61300; AAS61300; YP_1050
GeneIDi1173954
KEGGiype:YPO1106
ypk:y3074
ypm:YP_1050
PATRICifig|214092.21.peg.1398

Similar proteinsi

Entry informationi

Entry nameiNADK_YERPE
AccessioniPrimary (citable) accession number: Q8ZH09
Secondary accession number(s): Q0WHT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health