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Protein

NAD kinase

Gene

nadK

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74Proton acceptorUniRule annotation1
Binding sitei159NADUniRule annotation1
Binding sitei176NADUniRule annotation1
Sitei176Responsible for conferring strict specificity to NADBy similarity1
Binding sitei178NADUniRule annotation1
Binding sitei186NAD; via carbonyl oxygenUniRule annotation1
Binding sitei248NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 75NADUniRule annotation2
Nucleotide bindingi148 – 149NADUniRule annotation2
Nucleotide bindingi189 – 194NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:YPO1106, y3074, YP_1050
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001206951 – 293NAD kinaseAdd BLAST293

Proteomic databases

PRIDEiQ8ZH09.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ8ZH09. 1 interactor.
STRINGi187410.y3074.

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Helixi21 – 33Combined sources13
Beta strandi37 – 41Combined sources5
Helixi42 – 48Combined sources7
Beta strandi54 – 56Combined sources3
Helixi58 – 64Combined sources7
Beta strandi66 – 72Combined sources7
Helixi74 – 84Combined sources11
Turni85 – 88Combined sources4
Beta strandi90 – 94Combined sources5
Beta strandi96 – 98Combined sources3
Helixi107 – 109Combined sources3
Helixi110 – 119Combined sources10
Beta strandi122 – 135Combined sources14
Beta strandi142 – 156Combined sources15
Beta strandi161 – 167Combined sources7
Beta strandi170 – 183Combined sources14
Helixi186 – 189Combined sources4
Helixi191 – 194Combined sources4
Beta strandi205 – 213Combined sources9
Beta strandi222 – 225Combined sources4
Beta strandi230 – 235Combined sources6
Beta strandi241 – 245Combined sources5
Beta strandi251 – 253Combined sources3
Beta strandi258 – 273Combined sources16
Helixi278 – 286Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HAOX-ray2.55A/B1-293[»]
ProteinModelPortaliQ8ZH09.
SMRiQ8ZH09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
HOGENOMiHOG000227221.
KOiK00858.
OMAiKLHWHEY.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZH09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNRRFDCIG IVGHPRHPAA LATHEILYHW LKARGYAVMV EQQIAHDLNL
60 70 80 90 100
TDAITGSLAD IGQKADLAVV VGGDGNMLGA ARVLARYDIK VIGVNRGNLG
110 120 130 140 150
FLTDLDPDNA LQQLSDVLEG EYLSEQRFLL ETHVRRTNQQ SRISTAINEV
160 170 180 190 200
VLHPGKVAHM IEFEVYIDDR FAFSQRSDGL IIATPTGSTA YSLSAGGPIL
210 220 230 240 250
TPTLDAIVLV PMFPHTLTAR PLVISSSSTI RLKFSHITSD LEISCDSQIA
260 270 280 290
LPIQEGEEVL IRRSDFHLNL IHPKDYSYFN TLSTKLGWSK KLF
Length:293
Mass (Da):32,398
Last modified:March 1, 2002 - v1
Checksum:iC0231F341E8F4A1E
GO

Sequence cautioni

The sequence AAS61300 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19772.1.
AE009952 Genomic DNA. Translation: AAM86624.1.
AE017042 Genomic DNA. Translation: AAS61300.1. Different initiation.
PIRiAB0136.
RefSeqiWP_002210719.1. NZ_LQBA01000014.1.
YP_002346149.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM86624; AAM86624; y3074.
AAS61300; AAS61300; YP_1050.
GeneIDi1173954.
KEGGiype:YPO1106.
ypk:y3074.
ypm:YP_1050.
PATRICi18591493. VBIYerPes7843_1474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19772.1.
AE009952 Genomic DNA. Translation: AAM86624.1.
AE017042 Genomic DNA. Translation: AAS61300.1. Different initiation.
PIRiAB0136.
RefSeqiWP_002210719.1. NZ_LQBA01000014.1.
YP_002346149.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HAOX-ray2.55A/B1-293[»]
ProteinModelPortaliQ8ZH09.
SMRiQ8ZH09.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8ZH09. 1 interactor.
STRINGi187410.y3074.

Proteomic databases

PRIDEiQ8ZH09.

Protocols and materials databases

DNASUi1148021.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM86624; AAM86624; y3074.
AAS61300; AAS61300; YP_1050.
GeneIDi1173954.
KEGGiype:YPO1106.
ypk:y3074.
ypm:YP_1050.
PATRICi18591493. VBIYerPes7843_1474.

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
HOGENOMiHOG000227221.
KOiK00858.
OMAiKLHWHEY.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADK_YERPE
AccessioniPrimary (citable) accession number: Q8ZH09
Secondary accession number(s): Q0WHT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.