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Protein

Probable endonuclease 4

Gene

nfo

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Zinc 1UniRule annotation
Metal bindingi109 – 1091Zinc 1UniRule annotation
Metal bindingi145 – 1451Zinc 1UniRule annotation
Metal bindingi145 – 1451Zinc 2UniRule annotation
Metal bindingi179 – 1791Zinc 2UniRule annotation
Metal bindingi182 – 1821Zinc 3UniRule annotation
Metal bindingi216 – 2161Zinc 2UniRule annotation
Metal bindingi229 – 2291Zinc 3UniRule annotation
Metal bindingi231 – 2311Zinc 3UniRule annotation
Metal bindingi261 – 2611Zinc 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYPES214092:GKDD-1295-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:YPO1306, y2879, YP_1286
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Probable endonuclease 4PRO_0000190890Add
BLAST

Proteomic databases

PRIDEiQ8ZGJ1.

Interactioni

Protein-protein interaction databases

IntActiQ8ZGJ1. 1 interaction.
STRINGi187410.y2879.

Structurei

3D structure databases

ProteinModelPortaliQ8ZGJ1.
SMRiQ8ZGJ1. Positions 1-278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224893.
KOiK01151.
OMAiMKYVGAH.
OrthoDBiEOG6Z0QCM.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZGJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVGAHVSA AGGVDQAVIR AHELEATAFA LFTKNQRQWR AAPLAEDVIE
60 70 80 90 100
KFKLACEKYG YTSAQILPHD SYLINLGHPV TEALEKSREA FIDELVRCQQ
110 120 130 140 150
LGLSLLNFHP GSHLLQIDED QCLARIAESI NIALDATEGV TAVIENTAGQ
160 170 180 190 200
GSNLGFKFEH LAAIIERVED KSRVGVCIDT CHAFAAGYDL RTEEDCEHTF
210 220 230 240 250
AALGKIVGFQ YLRGMHLNDA KSEFNSRVDR HHSLGEGNIG KTVFSYIMRD
260 270 280
SRFDNIPLIL ETVNMDIWAE EIAWLKSQAE IEPSL
Length:285
Mass (Da):31,672
Last modified:March 1, 2002 - v1
Checksum:i07BCA59BB4FDAA9D
GO

Sequence cautioni

The sequence AAM86430.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAS61529.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19959.1.
AE009952 Genomic DNA. Translation: AAM86430.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS61529.1. Different initiation.
PIRiAE0159.
RefSeqiWP_002208791.1. NZ_LIXY01000012.1.
YP_002346331.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM86430; AAM86430; y2879.
AAS61529; AAS61529; YP_1286.
GeneIDi1174148.
KEGGiype:YPO1306.
ypj:CH55_1242.
ypk:y2879.
ypl:CH46_3824.
ypm:YP_1286.
ypv:BZ15_2249.
ypw:CH59_538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19959.1.
AE009952 Genomic DNA. Translation: AAM86430.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS61529.1. Different initiation.
PIRiAE0159.
RefSeqiWP_002208791.1. NZ_LIXY01000012.1.
YP_002346331.1. NC_003143.1.

3D structure databases

ProteinModelPortaliQ8ZGJ1.
SMRiQ8ZGJ1. Positions 1-278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8ZGJ1. 1 interaction.
STRINGi187410.y2879.

Proteomic databases

PRIDEiQ8ZGJ1.

Protocols and materials databases

DNASUi1147826.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM86430; AAM86430; y2879.
AAS61529; AAS61529; YP_1286.
GeneIDi1174148.
KEGGiype:YPO1306.
ypj:CH55_1242.
ypk:y2879.
ypl:CH46_3824.
ypm:YP_1286.
ypv:BZ15_2249.
ypw:CH59_538.

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224893.
KOiK01151.
OMAiMKYVGAH.
OrthoDBiEOG6Z0QCM.

Enzyme and pathway databases

BioCyciYPES214092:GKDD-1295-MONOMER.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiEND4_YERPE
AccessioniPrimary (citable) accession number: Q8ZGJ1
Secondary accession number(s): Q0WHA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: March 1, 2002
Last modified: May 11, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.