Reviewed, UniProtKB/Swiss-Prot Q8ZFV8 (LLDD_YERPE)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase [cytochrome]
    EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	YPO1569, y2596, YP_1457

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP MF_01559
Cofactor	FMN By similarity.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	L-lactate dehydrogenase [cytochrome] HAMAP MF_01559		PRO_0000206359
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8ZFV8-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: ECC708A08D5E4634	FASTA	381	41,259
	10 20 30 40 50 60 MIISASTDYR AAAQRKLPPF LFHYIDGGAY NEQTLRRNTA DLADIALRQR VLKNMSELSL 70 80 90 100 110 120 ETQLFGETQA MPVVLGPVGL SGMYARRGEV QAARAADKKG IPFTLSTLSV CPIEEVAPAI 130 140 150 160 170 180 ARPMWFQLYV LKDRGFMRNA LTRAQAAGVK TLVFTVDMPV PGARYRDAHS GMSGPNAAAR 190 200 210 220 230 240 RLLQAIAHPQ WAWDVGLNGK PHDLGNISAY LGKPTTLEDY MGWIATNFDP SISWKDLEWV 250 260 270 280 290 300 REFWQGPMII KGILDPEDAK DAVKFGADGI VVSNHGGRQL DGVLSTARAL PAIADAVKGD 310 320 330 340 350 360 ITILADSGIR TGLDVVRMIA LGADSVLLGR AFVYALATAG EAGVINLLTL IEQEMRVAMT 370 380 LTGAKRIADI NRDSLAVSER G

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL590842 Genomic DNA. Translation: CAL20214.1. AE009952 Genomic DNA. Translation: AAM86151.1. AE017042 Genomic DNA. Translation: AAS61696.1.
PIR	AD0191.
RefSeq	NP_669900.1. NP_992819.1. YP_002346582.1.
3D structure databases
HSSP	HSSP built from PDB template 1GOX based on UniProtKB P05414.
ModBase	Search...
Genome annotation databases
GeneID	1147543. 1174407. 2767186.
GenomeReviews	Gene locus y2596 in contig AE009952_GR. Gene locus YP_1457 in contig AE017042_GR. Gene locus YPO1569 in contig AL590842_GR.
KEGG	ype:YPO1569. ypk:y2596. ypm:YP_1457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZFV8.
OMA	Q8ZFV8. NFDPSIG.
Enzyme and pathway databases
BioCyc	YPES187410:Y2596-MON. YPES214092:YPO1569-MON. YPES229193:YP1457-MON.
BRENDA	1.1.2.3. 142588.
Family and domain databases
HAMAP	MF_01559. [Tree]
InterPro	IPR012133. a-Hydoxy_acid_DH_FMN. IPR013785. Aldolase_TIM. IPR000262. FMN-dep_DH. IPR017934. FMN-dep_OHA_DH. IPR008259. FMN_hydac_DH_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_YERPE

Accession

Primary (citable) accession number: Q8ZFV8
Secondary accession number(s): Q0WGK6, Q74V74, Q7CHT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)