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Protein

Argininosuccinate synthase

Gene

argG

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argI), Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei99CitrullineBy similarity1
Binding sitei129ATP; via amide nitrogenBy similarity1
Binding sitei131AspartateBy similarity1
Binding sitei131ATPBy similarity1
Binding sitei135AspartateBy similarity1
Binding sitei135CitrullineBy similarity1
Binding sitei136AspartateBy similarity1
Binding sitei136ATPBy similarity1
Binding sitei139CitrullineBy similarity1
Binding sitei192CitrullineBy similarity1
Binding sitei194ATPBy similarity1
Binding sitei201CitrullineBy similarity1
Binding sitei203CitrullineBy similarity1
Binding sitei280CitrullineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:YPO1570, y2595, YP_1458
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001487102 – 455Argininosuccinate synthaseAdd BLAST454

Proteomic databases

PRIDEiQ8ZFV7.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ8ZFV7. 1 interactor.
STRINGi187410.y2595.

Structurei

3D structure databases

ProteinModelPortaliQ8ZFV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230094.
KOiK01940.
OMAiAFHIRSG.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2. 1 hit.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8ZFV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTILKHLPI NQRVGIAFSG GLDTSAALLW MQKKGAIPYA YTANLGQPDE
60 70 80 90 100
EDYEAIPRKA MEYGAEKARL IDCRKQLVAE GIAAIQCGAF HNTTAGVTYF
110 120 130 140 150
NTTPLGRAVT GTMLVAAMKE DDVNIWGDGS TYKGNDIERF YRYGLLTNAE
160 170 180 190 200
LKIYKPWLDT DFIDELGGRH EMSEFMIQSG FDYKMSTEKA YSTDSNMLGA
210 220 230 240 250
THEAKDLEFL NSSVKIVNPI MGVKFWDENV VVKAEEVTVR FERGYPVALN
260 270 280 290 300
GVVFDDSVEL MMEANRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGMALL
310 320 330 340 350
HIAYERLLTG IHNEDTIEQY HANGRVLGRL LYQGRWFDPQ ALMLRDSIQR
360 370 380 390 400
WVASEITGEV TLELRRGNDY SILNTVSDNL TYKPERLTME KGDSVFSPDD
410 420 430 440 450
RIGQLTMRNL DITDTREKLF NYVETGLLTS SAATGLPQVD NNNLSSGRGL

QDKRQ
Length:455
Mass (Da):51,009
Last modified:January 23, 2007 - v3
Checksum:i7B1A2B828CDAE057
GO

Sequence cautioni

The sequence AAM86150 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS61697 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL20215.1.
AE009952 Genomic DNA. Translation: AAM86150.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS61697.1. Different initiation.
PIRiAE0191.
RefSeqiWP_002211920.1. NZ_LQBA01000060.1.
YP_002346583.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM86150; AAM86150; y2595.
AAS61697; AAS61697; YP_1458.
GeneIDi1174408.
KEGGiype:YPO1570.
ypj:CH55_968.
ypk:y2595.
ypl:CH46_3556.
ypm:YP_1458.
ypv:BZ15_1982.
ypw:CH59_256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL20215.1.
AE009952 Genomic DNA. Translation: AAM86150.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS61697.1. Different initiation.
PIRiAE0191.
RefSeqiWP_002211920.1. NZ_LQBA01000060.1.
YP_002346583.1. NC_003143.1.

3D structure databases

ProteinModelPortaliQ8ZFV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8ZFV7. 1 interactor.
STRINGi187410.y2595.

Proteomic databases

PRIDEiQ8ZFV7.

Protocols and materials databases

DNASUi1147542.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM86150; AAM86150; y2595.
AAS61697; AAS61697; YP_1458.
GeneIDi1174408.
KEGGiype:YPO1570.
ypj:CH55_968.
ypk:y2595.
ypl:CH46_3556.
ypm:YP_1458.
ypv:BZ15_1982.
ypw:CH59_256.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230094.
KOiK01940.
OMAiAFHIRSG.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2. 1 hit.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_YERPE
AccessioniPrimary (citable) accession number: Q8ZFV7
Secondary accession number(s): Q0WGK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.