Q8ZFL4 (ALR2_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase 2
EC=5.1.1.1

Gene names

Name:	alr2
Ordered Locus Names:	YPO1687, y1849, YP_1816

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.
Caution	The gene coding for this protein seems to be defective in strain 91001 where it is interrupted by the insertion of an IS285 element.
Sequence caution	The sequence AAM85416.1 differs from that shown. Reason: Erroneous initiation. The sequence CAL20330.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Alanine racemase 2 HAMAP-Rule MF_01201		PRO_0000114603
Sites
	Active site	61	1	Proton acceptor; specific for D-alanine By similarity
	Active site	286	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	334	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	61	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZFL4 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 41516FB5C4EE750F	FASTA	394	42,912
	10 20 30 40 50 60 MVACSQPVSK NHLSLTSLSA NAQQPVVNNA WLEISQGALD FNTKKMLTLL DNKSTLCAIL 70 80 90 100 110 120 KGDAYGHDLT LVTPVMLKNN VQCIGVASNQ ELKTVRDLGF TGQLIRVRSA TLKEMQQAMA 130 140 150 160 170 180 YDVEELIGDK TVAEQLNNIA KLNGKVLRIH LALNSAGMSR NGLEVSKARG LNDAKTIAGL 190 200 210 220 230 240 KNLTIVGIMS HYPVEDASEI KADLARFQQQ AKDVIAVTGL KREKIKLHVA NTFATLAVPD 250 260 270 280 290 300 SWLDMVRVGG VFYGDTIAST EYKRVMTFKS NIASLNNYPK GGTVGYDRTY TLKRDSLLAN 310 320 330 340 350 360 IPVGYADGYR RVFSNAGHVI IQGQRLPVLG KTSMNTVIVD VTDLKKVSLG DEVVLFGKQG 370 380 390 NAEIQAEEIE DLSGALFTEM SILWGATNKR ILVD

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL20330.1. Different initiation. AE009952 Genomic DNA. Translation: AAM85416.1. Different initiation. AE017042 Genomic DNA. No translation available.
PIR	AG0205.
RefSeq	NP_669165.1. NC_004088.1. YP_002346690.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZFL4.
ModBase	Search...
Protein-protein interaction databases
STRING	214092.YPO1687.
Protocols and materials databases
DNASU	1146796.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM85416; AAM85416; y1849.
GeneID	1146796. 1174524.
KEGG	ype:YPO1687. ypk:y1849.
PATRIC	18592836. VBIYerPes7843_2136.
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000279190.
KO	K01775.
OMA	NTVGYDR.
ProtClustDB	PRK13340.
Enzyme and pathway databases
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_YERPE

Accession

Primary (citable) accession number: Q8ZFL4
Secondary accession number(s): Q0WG98

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program