Q8ZES9 (LCFA_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain-fatty-acid--CoA ligase
EC=6.2.1.3

Alternative name(s):
Long-chain acyl-CoA synthetase

Gene names

Name:	fadD
Ordered Locus Names:	YPO2074, y2236, YP_1917

Organism

Yersinia pestis

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Subunit structure	Homodimer Probable.
Subcellular location	Membrane; Peripheral membrane protein Potential. Note: Partially membrane-associated Potential.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence AAM85796.1 differs from that shown. Reason: Erroneous initiation. The sequence AAS62135.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain fatty acid-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Long-chain-fatty-acid--CoA ligase		PRO_0000193130
Regions
	Nucleotide binding	213 – 224	12	ATP Probable
Natural variations
	Natural variant	504	1	N → K in strain: KIM5 and 91001.

Sequences

	Sequence		Length	Mass (Da)
	Q8ZES9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 812A872B3713D3FD	FASTA	562	62,639
	10 20 30 40 50 60 MEKVWLKRYP ADVPAEIDPD RYSSLIEMFE NAALRYADQP AFINMGEVMT FRKLEERSRA 70 80 90 100 110 120 FAAYLQQGLG LQKGDRVALM MPNLLQYPIA LFGVLRAGMI VVNVNPLYTP RELEHQLSDS 130 140 150 160 170 180 GAVAIVIVSN FAHTLEKVVF KTQVRHVILT RMGDQLSAAK GTLVNFVVKY IKRLVPKYYL 190 200 210 220 230 240 PDAISFRTVL QKGRRMQYVK PDVINTDTAF LQYTGGTTGV AKGAILTHRN MQSNLEQAKA 250 260 270 280 290 300 AYAPLLQPGR DLVVTALPLY HIFALTVNCL LFIELGGRSL LITNPRDIPG MVKELSRYPF 310 320 330 340 350 360 TAITGVNTLF NALLNNEEFT HLDFSTLRLS VGGGMPVQKA VAEKWETLTG KHLLEGYGLT 370 380 390 400 410 420 ECSPLVTGNP YDLKHYSGSI GLPVPSTDVR LRDDDGNDVE LGKPGELWVR GPQVMLGYWQ 430 440 450 460 470 480 RPDATDDVLK DGWLATGDIA TMDEDGFLRI VDRKKDMILV SGFNVYPNEI EEVVALHAKV 490 500 510 520 530 540 LESAVIGVPN EVSGEAVKVF VVKNDASLTP EELLTHCRRY LTGYKVPKIV EFRDELPKSN 550 560 VGKILRRELR DEEVKVGTTD AA

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL20709.1. AE009952 Genomic DNA. Translation: AAM85796.1. Different initiation. AE017042 Genomic DNA. Translation: AAS62135.1. Different initiation.
PIR	AB0253.
RefSeq	NP_669545.1. NC_004088.1. NP_993258.1. NC_005810.1. YP_002347056.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZES9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1147183. 1174912. 2766250.
GenomeReviews	Gene locus y2236 in contig AE009952_GR. Gene locus YP_1917 in contig AE017042_GR. Gene locus YPO2074 in contig AL590842_GR.
KEGG	ype:YPO2074. ypk:y2236. ypm:YP_1917.
Phylogenomic databases
HOGENOM	HBG547964.
OMA	WAAVGEP.
ProtClustDB	PRK08974.
Enzyme and pathway databases
BioCyc	YPES187410:Y2236-MONOMER. YPES214092:YPO2074-MONOMER.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01897.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCFA_YERPE

Accession

Primary (citable) accession number: Q8ZES9
Secondary accession number(s): Q0WF82

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program