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Q8ZES9 (LCFA_YERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase

EC=6.2.1.3
Alternative name(s):
Long-chain acyl-CoA synthetase
Gene names
Name:fadD
Ordered Locus Names:YPO2074, y2236, YP_1917
OrganismYersinia pestis [Reference proteome] [HAMAP]
Taxonomic identifier632 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids By similarity.

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Subunit structure

Homodimer Probable.

Subcellular location

Membrane; Peripheral membrane protein Potential. Note: Partially membrane-associated Potential.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Sequence caution

The sequence AAM85796.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAS62135.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processFatty acid metabolism
Lipid metabolism
   Cellular componentMembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

long-chain fatty acid-CoA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 562562Long-chain-fatty-acid--CoA ligase
PRO_0000193130

Regions

Nucleotide binding213 – 22412ATP Probable

Natural variations

Natural variant5041N → K in strain: KIM5 and 91001.

Sequences

Sequence LengthMass (Da)Tools
Q8ZES9 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 812A872B3713D3FD

FASTA56262,639
        10         20         30         40         50         60 
MEKVWLKRYP ADVPAEIDPD RYSSLIEMFE NAALRYADQP AFINMGEVMT FRKLEERSRA 

        70         80         90        100        110        120 
FAAYLQQGLG LQKGDRVALM MPNLLQYPIA LFGVLRAGMI VVNVNPLYTP RELEHQLSDS 

       130        140        150        160        170        180 
GAVAIVIVSN FAHTLEKVVF KTQVRHVILT RMGDQLSAAK GTLVNFVVKY IKRLVPKYYL 

       190        200        210        220        230        240 
PDAISFRTVL QKGRRMQYVK PDVINTDTAF LQYTGGTTGV AKGAILTHRN MQSNLEQAKA 

       250        260        270        280        290        300 
AYAPLLQPGR DLVVTALPLY HIFALTVNCL LFIELGGRSL LITNPRDIPG MVKELSRYPF 

       310        320        330        340        350        360 
TAITGVNTLF NALLNNEEFT HLDFSTLRLS VGGGMPVQKA VAEKWETLTG KHLLEGYGLT 

       370        380        390        400        410        420 
ECSPLVTGNP YDLKHYSGSI GLPVPSTDVR LRDDDGNDVE LGKPGELWVR GPQVMLGYWQ 

       430        440        450        460        470        480 
RPDATDDVLK DGWLATGDIA TMDEDGFLRI VDRKKDMILV SGFNVYPNEI EEVVALHAKV 

       490        500        510        520        530        540 
LESAVIGVPN EVSGEAVKVF VVKNDASLTP EELLTHCRRY LTGYKVPKIV EFRDELPKSN 

       550        560 
VGKILRRELR DEEVKVGTTD AA 

« Hide

References

[1]"Genome sequence of Yersinia pestis, the causative agent of plague."
Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. expand/collapse author list , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.
Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CO-92 / Biovar Orientalis.
[2]"Genome sequence of Yersinia pestis KIM."
Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. expand/collapse author list , Matson J.S., Blattner F.R., Perry R.D.
J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KIM10+ / Biovar Mediaevalis.
[3]"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. expand/collapse author list , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590842 Genomic DNA. Translation: CAL20709.1.
AE009952 Genomic DNA. Translation: AAM85796.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS62135.1. Different initiation.
PIRAB0253.
RefSeqNP_669545.1. NC_004088.1.
NP_993258.1. NC_005810.1.
YP_002347056.1. NC_003143.1.

3D structure databases

ProteinModelPortalQ8ZES9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING214092.YPO2074.

Protocols and materials databases

DNASU1147183.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM85796; AAM85796; y2236.
AAS62135; AAS62135; YP_1917.
GeneID1147183.
1174912.
2766250.
KEGGype:YPO2074.
ypk:y2236.
ypm:YP_1917.

Phylogenomic databases

eggNOGCOG0318.
HOGENOMHOG000229983.
KOK01897.
OMAACIANTP.
OrthoDBEOG6MH5BV.

Enzyme and pathway databases

BioCycYPES214092:GKDD-2049-MONOMER.

Family and domain databases

InterProIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLCFA_YERPE
AccessionPrimary (citable) accession number: Q8ZES9
Secondary accession number(s): Q0WF82
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families